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- PDB-5hxn: Crystal Structure of Z,Z-Farnesyl Diphosphate Synthase (D71M and ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5hxn | ||||||
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Title | Crystal Structure of Z,Z-Farnesyl Diphosphate Synthase (D71M and E75A mutants) from the Wild Tomato Solanum habrochaites | ||||||
![]() | (2Z,6Z)-farnesyl diphosphate synthase, chloroplastic | ||||||
![]() | TRANSFERASE / prenyltransferase | ||||||
Function / homology | ![]() (2Z,6Z)-farnesyl diphosphate synthase / 2-cis,6-cis-farnesyl pyrophosphate synthase activity / plastid membrane organization / dehydrodolichyl diphosphate synthase activity / polyprenol biosynthetic process / polyprenyltransferase activity / chloroplast stroma / response to cold / magnesium ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lee, C.C. / Chan, Y.T. / Wang, A.H.J. | ||||||
![]() | ![]() Title: Crystal Structure and Potential Head-to-Middle Condensation Function of aZ,Z-Farnesyl Diphosphate Synthase. Authors: Chan, Y.T. / Ko, T.P. / Yao, S.H. / Chen, Y.W. / Lee, C.C. / Wang, A.H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 63 KB | Display | ![]() |
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PDB format | ![]() | 44.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 431.4 KB | Display | ![]() |
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Full document | ![]() | 432.6 KB | Display | |
Data in XML | ![]() | 11.5 KB | Display | |
Data in CIF | ![]() | 16.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5hxoC ![]() 5hxpC ![]() 1jp3S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | ( Mass: 26758.727 Da / Num. of mol.: 1 / Fragment: UNP RESIDUES 72-303 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: B8XA40, (2Z,6Z)-farnesyl diphosphate synthase |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.18 Å3/Da / Density % sol: 43.57 % |
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Crystal grow | Temperature: 291 K / Method: evaporation / pH: 8 Details: 70% 2-Methyl-2,4-pentanediol (MPD), 0.1 M Tris, pH 8.0. |
-Data collection
Diffraction | Mean temperature: 93 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jan 12, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.05→25 Å / Num. obs: 15386 / % possible obs: 97.4 % / Redundancy: 11 % / Rmerge(I) obs: 0.044 / Net I/σ(I): 52.3 |
Reflection shell | Resolution: 2.05→2.12 Å / Redundancy: 10 % / Rmerge(I) obs: 0.495 / Mean I/σ(I) obs: 4.3 / % possible all: 94.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1JP3 Resolution: 2.05→23.22 Å / Cor.coef. Fo:Fc: 0.946 / Cor.coef. Fo:Fc free: 0.916 / SU B: 4.696 / SU ML: 0.128 / Cross valid method: THROUGHOUT / ESU R: 0.218 / ESU R Free: 0.187 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 28.296 Å2
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Refinement step | Cycle: 1 / Resolution: 2.05→23.22 Å
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Refine LS restraints |
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