Component-ID: _ / Beg auth comp-ID: PRO / Beg label comp-ID: PRO / End auth comp-ID: HIS / End label comp-ID: HIS / Refine code: _ / Auth seq-ID: 738 - 982 / Label seq-ID: 11 - 255
Dom-ID
Ens-ID
Auth asym-ID
Label asym-ID
1
1
A
A
2
1
B
B
1
2
A
A
2
2
C
C
1
3
B
B
2
3
C
C
NCS ensembles :
ID
1
2
3
-
Components
#1: Protein
Mineralocorticoidreceptor / MR / Nuclear receptor subfamily 3 group C member 2
Mass: 29873.594 Da / Num. of mol.: 3 / Fragment: ligand-binding domain (UNP residues 732-984) / Mutation: C808S Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: NR3C2, MCR, MLR / Plasmid: pMAL_c5x / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) / References: UniProt: P08235
Mass: 18.015 Da / Num. of mol.: 260 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION
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Sample preparation
Crystal
Density Matthews: 2.22 Å3/Da / Density % sol: 44.53 %
Crystal grow
Temperature: 294 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 1uL of protein complex at 8-10mg protein/ml is mixed with an equal volume of reservoir solution composed of 100mM Tris-HCl pH=8.0, 200mM NaCl, 5% (v/v) glycerol, 16%(w/v) PEG3350 PH range: 7.5 - 8.5
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Data collection
Diffraction
Mean temperature: 100 K / Ambient temp details: CryoJet 7000