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- PDB-5f3q: Crystal structure of a noncanonical Dicer protein from Entamoeba ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5f3q | ||||||
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Title | Crystal structure of a noncanonical Dicer protein from Entamoeba histolytica | ||||||
![]() | Putative uncharacterized protein | ||||||
![]() | RNA BINDING PROTEIN / RNaseIII / Dicer / RNA processing / Entamoeba histolytica | ||||||
Function / homology | ribonuclease III activity / Ribonuclease III, endonuclease domain superfamily / RNA processing / metal ion binding / Uncharacterized protein![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Yu, X. / Gan, J.H. / Ma, J.B. | ||||||
![]() | ![]() Title: Structural and functional study of a noncanonical Dicer protein in Entamoeba histolytica Authors: Yu, X. / Gan, J.H. / Ma, J.B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 82.7 KB | Display | ![]() |
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PDB format | ![]() | 67 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 450.2 KB | Display | ![]() |
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Full document | ![]() | 452.8 KB | Display | |
Data in XML | ![]() | 14.7 KB | Display | |
Data in CIF | ![]() | 19.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 22778.357 Da / Num. of mol.: 2 / Fragment: UNP RESIDUES 2-193 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.24 Å3/Da / Density % sol: 45.12 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, hanging drop / Details: 1.26 M (NH4)2SO4, 0.1 M Hepes pH 7.8 / PH range: 7.5-7.9 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Oct 15, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→30 Å / Num. obs: 24145 / % possible obs: 99.1 % / Redundancy: 3.8 % / Net I/σ(I): 24.2 |
Reflection shell | Resolution: 2.1→2.18 Å |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→29.996 Å
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Refine LS restraints |
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LS refinement shell |
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