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- PDB-5eqw: Structure of the major structural protein D135 of Acidianus taile... -

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Basic information

Entry
Database: PDB / ID: 5eqw
TitleStructure of the major structural protein D135 of Acidianus tailed spindle virus (ATSV)
ComponentsPutative major coat protein
KeywordsSTRUCTURAL PROTEIN / Archaeal virus / four helix bundle / large tailed spindle virus
Function / homologyRIP/P131 family / viral capsid / NITRATE ION / Putative major coat protein
Function and homology information
Biological speciesAcidianus tailed spindle virus
MethodX-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 1.679 Å
AuthorsHochstein, R.A. / Lintner, N.G. / Young, M.J. / Lawrence, C.M.
Funding support United States, 3items
OrganizationGrant numberCountry
National Science Foundation (NSF, United States)DEB-4W4596 United States
National Science Foundation (NSF, United States)MCB-1413534 United States
National Science Foundation (NSF, United States)MCB-0920312 United States
CitationJournal: Proc. Natl. Acad. Sci. U.S.A. / Year: 2018
Title: Structural studies ofAcidianustailed spindle virus reveal a structural paradigm used in the assembly of spindle-shaped viruses.
Authors: Hochstein, R. / Bollschweiler, D. / Dharmavaram, S. / Lintner, N.G. / Plitzko, J.M. / Bruinsma, R. / Engelhardt, H. / Young, M.J. / Klug, W.S. / Lawrence, C.M.
History
DepositionNov 13, 2015Deposition site: RCSB / Processing site: RCSB
Revision 1.0Nov 16, 2016Provider: repository / Type: Initial release
Revision 1.1Sep 27, 2017Group: Author supporting evidence / Derived calculations / Category: pdbx_audit_support / pdbx_struct_oper_list
Item: _pdbx_audit_support.funding_organization / _pdbx_struct_oper_list.symmetry_operation
Revision 1.2Feb 14, 2018Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.title / _citation.year
Revision 1.3Apr 18, 2018Group: Data collection / Database references / Category: citation / citation_author
Item: _citation.journal_abbrev / _citation.journal_volume ..._citation.journal_abbrev / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.name
Revision 1.4Nov 27, 2019Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Putative major coat protein
B: Putative major coat protein
C: Putative major coat protein
D: Putative major coat protein
E: Putative major coat protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)81,68615
Polymers81,0665
Non-polymers62010
Water11,890660
1
A: Putative major coat protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)16,3373
Polymers16,2131
Non-polymers1242
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
2
B: Putative major coat protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)16,3373
Polymers16,2131
Non-polymers1242
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
3
C: Putative major coat protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)16,3373
Polymers16,2131
Non-polymers1242
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
4
D: Putative major coat protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)16,3373
Polymers16,2131
Non-polymers1242
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
5
E: Putative major coat protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)16,3373
Polymers16,2131
Non-polymers1242
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
Unit cell
Length a, b, c (Å)97.780, 41.672, 97.690
Angle α, β, γ (deg.)90.00, 108.64, 90.00
Int Tables number3
Space group name H-MP121
Components on special symmetry positions
IDModelComponents
11B-394-

HOH

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Components

#1: Protein
Putative major coat protein


Mass: 16213.122 Da / Num. of mol.: 5
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Acidianus tailed spindle virus / Gene: ATSV_D135 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A125SJ78
#2: Chemical
ChemComp-NO3 / NITRATE ION


Mass: 62.005 Da / Num. of mol.: 10 / Source method: obtained synthetically / Formula: NO3
#3: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 660 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.33 Å3/Da / Density % sol: 47.28 %
Crystal growTemperature: 295 K / Method: vapor diffusion, hanging drop / pH: 2.6
Details: Sodium nitrate, sodium acetate, glycerol, acetonitrile

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: SSRL / Beamline: BL9-1 / Wavelength: 0.95369 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: Jan 19, 2011
RadiationProtocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.95369 Å / Relative weight: 1
ReflectionResolution: 1.679→50 Å / Num. obs: 84697 / % possible obs: 98.7 % / Redundancy: 3.5 % / Rsym value: 0.066 / Net I/σ(I): 16.26

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Processing

Software
NameVersionClassification
PHENIX1.9_1692refinement
Coot0.8.1model building
SCALEPACKdata scaling
SOLVEphasing
DENZOdata reduction
RefinementMethod to determine structure: MAD / Resolution: 1.679→38.005 Å / SU ML: 0.18 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 21.58 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.216 4217 4.98 %
Rwork0.189 --
obs0.1904 84672 98.51 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 1.679→38.005 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4759 0 40 660 5459
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0064978
X-RAY DIFFRACTIONf_angle_d1.0036757
X-RAY DIFFRACTIONf_dihedral_angle_d12.7091929
X-RAY DIFFRACTIONf_chiral_restr0.044731
X-RAY DIFFRACTIONf_plane_restr0.006931
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.6791-1.69820.27091130.24032075X-RAY DIFFRACTION79
1.6982-1.71820.24631250.23382478X-RAY DIFFRACTION91
1.7182-1.73910.26451250.23452644X-RAY DIFFRACTION95
1.7391-1.76110.24771330.22532556X-RAY DIFFRACTION97
1.7611-1.78430.23641230.22912710X-RAY DIFFRACTION99
1.7843-1.80880.25751430.2132729X-RAY DIFFRACTION100
1.8088-1.83460.24361420.21162634X-RAY DIFFRACTION100
1.8346-1.8620.21231320.21092693X-RAY DIFFRACTION100
1.862-1.89110.26761390.20132741X-RAY DIFFRACTION100
1.8911-1.92210.21751420.19672672X-RAY DIFFRACTION100
1.9221-1.95520.21881400.18722701X-RAY DIFFRACTION100
1.9552-1.99080.19211360.17342735X-RAY DIFFRACTION100
1.9908-2.02910.1921490.17872647X-RAY DIFFRACTION100
2.0291-2.07050.18741450.18012741X-RAY DIFFRACTION100
2.0705-2.11550.22681290.1872713X-RAY DIFFRACTION100
2.1155-2.16470.20411540.18672713X-RAY DIFFRACTION100
2.1647-2.21880.21221340.19052716X-RAY DIFFRACTION100
2.2188-2.27880.19651480.18142685X-RAY DIFFRACTION100
2.2788-2.34580.19811490.17752715X-RAY DIFFRACTION100
2.3458-2.42160.22871480.18732717X-RAY DIFFRACTION100
2.4216-2.50810.24161420.19222705X-RAY DIFFRACTION100
2.5081-2.60850.19651590.19032734X-RAY DIFFRACTION100
2.6085-2.72720.2571250.19652749X-RAY DIFFRACTION100
2.7272-2.87090.20871370.18832704X-RAY DIFFRACTION100
2.8709-3.05070.21161380.1952747X-RAY DIFFRACTION100
3.0507-3.28610.22771470.18612705X-RAY DIFFRACTION100
3.2861-3.61660.22041740.17992721X-RAY DIFFRACTION100
3.6166-4.13940.20291440.16082762X-RAY DIFFRACTION100
4.1394-5.2130.20591500.17392764X-RAY DIFFRACTION99
5.213-38.01440.2031520.21262849X-RAY DIFFRACTION99
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
14.1026-3.1887-0.13175.06051.19513.00170.05360.22060.20370.0048-0.1196-0.2092-0.02340.12270.03410.1209-0.0283-0.04210.15510.04370.097429.383266.400227.6967
23.43141.40921.64562.6530.02253.06360.21280.3765-0.4191-0.855-0.0307-0.18810.36170.1272-0.19020.32750.0115-0.09290.2322-0.0190.179424.51157.536619.4323
33.8287-2.9325-2.38863.77533.76464.02950.0966-0.0558-0.3495-0.1488-0.09750.58320.0352-0.31820.08060.1504-0.0184-0.07520.19790.03740.227417.699960.204128.7587
42.1309-0.31870.72664.4439-0.76494.2792-0.2203-0.02260.16150.13410.08760.1855-0.2724-0.20270.1450.17510.0359-0.0470.12330.00120.144520.015277.868728.9792
54.5404-4.1455-2.53084.99354.37475.2862-0.0814-0.1393-0.23360.30450.08910.16090.141-0.11590.05620.1732-0.0294-0.03020.16430.05830.128324.89158.338536.3544
66.74275.20641.58814.55850.67569.1392-0.18350.63450.0345-0.35660.2133-0.8248-0.00860.4593-0.01640.12110.0272-0.00360.19730.01430.221639.601755.926827.0757
73.0078-1.8358-1.10494.15371.09352.08050.09810.20330.1977-0.2619-0.0302-0.3622-0.0888-0.0005-0.06190.0794-0.00380.03560.1580.00470.113949.524649.622334.1415
85.45332.60381.74154.30580.77382.8981-0.10070.5044-0.4925-0.79530.20240.03410.3020.031-0.14680.1581-0.0209-0.01350.2335-0.00590.16540.757640.808730.3975
90.6136-1.4136-1.63394.4396.0877.9077-0.069-0.1266-0.19850.0174-0.24060.36590.0953-0.26510.36420.08830.00310.0250.2007-0.00110.186340.651843.381141.9035
105.1371-0.51971.59424.4734-0.66533.8602-0.0290.08950.3693-0.07810.0305-0.0343-0.00780.01060.00820.07220.01040.01030.0854-0.00640.071142.776461.157940.5033
113.23280.38710.16718.1345.07375.424-0.167-0.2231-0.23510.3231-0.0588-0.06880.269-0.12910.22790.08860.0230.00140.13180.04380.110351.003341.867343.8741
128.70025.39-2.01934.2138-3.42767.56150.02110.93110.4109-0.62170.3529-0.3559-0.145-0.0522-0.43860.23880.02350.1110.2553-0.01990.23957.728739.754527.3868
132.4571-0.1510.07825.64173.34673.3876-0.00390.17960.1355-0.3265-0.0319-0.1053-0.15620.050.05160.14250.0240.07880.17820.03390.173469.572633.02727.6329
144.97763.1676-0.08482.3308-0.61213.5081-0.13640.3193-0.5121-0.3274-0.15520.03180.5816-0.35880.30250.2248-0.01220.11340.2416-0.05030.31360.346524.108329.75
153.62953.85572.63247.29163.69113.0997-0.1289-0.4373-0.02661.0106-0.30540.53020.498-0.25330.41890.290.0360.10910.2234-0.00050.267.297726.839939.0931
165.9149-0.60180.95685.6951-0.52442.41560.07-0.18470.43170.17530.0835-0.3825-0.05220.0946-0.15480.14150.01060.03580.125-0.0410.191967.953644.490636.8544
176.56934.26793.68414.98785.46137.5143-0.2759-0.4093-0.05680.17530.063-0.32070.11570.02080.08780.22530.07660.05240.20980.01680.261176.693225.038334.3521
189.4344.9744-4.49795.1316-6.39629.13140.08520.72280.3062-0.44240.08840.1346-0.3537-0.5409-0.23180.2390.03110.1010.229-0.01210.201272.001322.668717.6833
192.00841.42691.28065.6983.45364.0544-0.00380.11870.0625-0.2188-0.06520.2332-0.1804-0.2560.05810.10440.04110.03530.14820.01470.102581.560116.430910.3095
202.80712.2758-1.35212.778-1.37734.7236-0.10310.1344-0.14280.1004-0.02510.72570.5662-0.46950.16450.2351-0.01910.10370.2473-0.00690.249975.49827.59817.5984
216.37275.14821.96384.1371.67751.56710.1749-0.4206-0.00530.6835-0.24730.16610.2576-0.05880.0420.26960.04310.03590.17650.00250.092886.539910.20921.0477
223.9668-0.9108-0.70384.6303-0.4352.55350.1037-0.08860.17810.07440.0653-0.0625-0.2170.0057-0.16010.1347-0.00340.0490.0964-0.0360.147785.839527.924118.726
235.75195.92761.80679.15092.55963.15840.1897-0.084-0.0990.2833-0.0285-0.24010.30020.0988-0.17910.14590.06120.03520.14230.0040.10291.25848.4111.5609
248.2684-3.18082.32683.49570.95053.1884-0.19450.20260.0794-0.66180.30360.5596-0.3343-0.6608-0.2090.220.0064-0.02910.22890.00140.147477.60275.96910.8962
251.76110.59541.01162.08040.50162.0035-0.11560.04810.1758-0.26570.00970.3529-0.0903-0.15790.02640.12930.0226-0.07580.17630.00320.138280.841-0.4021-10.8138
263.4787-0.11370.28142.8968-0.92164.33760.0948-0.3723-0.40120.3227-0.05480.41270.4196-0.378-0.05830.1946-0.0218-0.00110.20810.02110.128580.2241-9.1822-1.2342
276.93725.0997-1.35493.7035-1.28741.00940.3052-0.184-0.32170.253-0.208-0.02910.16340.1413-0.08460.1597-0.0011-0.07340.16970.00550.128991.2106-6.5947-5.2014
282.5958-0.6828-1.3574.71350.33495.0279-0.07720.03140.1135-0.2044-0.01080.1249-0.3724-0.02510.07980.0962-0.0225-0.03090.097-0.00660.092389.128911.2107-6.7336
292.36371.7592-2.41682.2996-2.88414.1088-0.0201-0.0916-0.1819-0.37410.0287-0.11650.0250.2018-0.03580.19870.0182-0.02690.1788-0.04520.174489.441-8.3402-15.6268
305.966-4.26773.54255.3702-4.56713.9546-0.2191-0.5693-0.0197-0.24540.42630.8022-0.2182-0.8871-0.03210.2612-0.0051-0.12920.28450.00030.274671.8151-10.1394-16.3916
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 10 through 30 )
2X-RAY DIFFRACTION2chain 'A' and (resid 31 through 49 )
3X-RAY DIFFRACTION3chain 'A' and (resid 50 through 69 )
4X-RAY DIFFRACTION4chain 'A' and (resid 70 through 97 )
5X-RAY DIFFRACTION5chain 'A' and (resid 98 through 118 )
6X-RAY DIFFRACTION6chain 'A' and (resid 119 through 131 )
7X-RAY DIFFRACTION7chain 'B' and (resid 10 through 30 )
8X-RAY DIFFRACTION8chain 'B' and (resid 31 through 49 )
9X-RAY DIFFRACTION9chain 'B' and (resid 50 through 69 )
10X-RAY DIFFRACTION10chain 'B' and (resid 70 through 97 )
11X-RAY DIFFRACTION11chain 'B' and (resid 98 through 118 )
12X-RAY DIFFRACTION12chain 'B' and (resid 119 through 132 )
13X-RAY DIFFRACTION13chain 'C' and (resid 10 through 30 )
14X-RAY DIFFRACTION14chain 'C' and (resid 31 through 49 )
15X-RAY DIFFRACTION15chain 'C' and (resid 50 through 69 )
16X-RAY DIFFRACTION16chain 'C' and (resid 70 through 97 )
17X-RAY DIFFRACTION17chain 'C' and (resid 98 through 118 )
18X-RAY DIFFRACTION18chain 'C' and (resid 119 through 131 )
19X-RAY DIFFRACTION19chain 'D' and (resid 10 through 30 )
20X-RAY DIFFRACTION20chain 'D' and (resid 31 through 49 )
21X-RAY DIFFRACTION21chain 'D' and (resid 50 through 69 )
22X-RAY DIFFRACTION22chain 'D' and (resid 70 through 97 )
23X-RAY DIFFRACTION23chain 'D' and (resid 98 through 118 )
24X-RAY DIFFRACTION24chain 'D' and (resid 119 through 131 )
25X-RAY DIFFRACTION25chain 'E' and (resid 10 through 30 )
26X-RAY DIFFRACTION26chain 'E' and (resid 31 through 49 )
27X-RAY DIFFRACTION27chain 'E' and (resid 50 through 69 )
28X-RAY DIFFRACTION28chain 'E' and (resid 70 through 97 )
29X-RAY DIFFRACTION29chain 'E' and (resid 98 through 118 )
30X-RAY DIFFRACTION30chain 'E' and (resid 119 through 132 )

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