- PDB-5e78: Crystal structure of P450 BM3 heme domain variant complexed with ... -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 5.0E+78
Title
Crystal structure of P450 BM3 heme domain variant complexed with Co(III)Sep
Components
Bifunctional P-450/NADPH-P450 reductase
Keywords
OXIDOREDUCTASE / BM3 / P450 / mediated electron transport / Co(III)Sep
Function / homology
Function and homology information
NADPH-hemoprotein reductase / NADPH-hemoprotein reductase activity / oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen / unspecific monooxygenase / aromatase activity / metabolic process / response to hormone / FMN binding / flavin adenine dinucleotide binding / iron ion binding ...NADPH-hemoprotein reductase / NADPH-hemoprotein reductase activity / oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen / unspecific monooxygenase / aromatase activity / metabolic process / response to hormone / FMN binding / flavin adenine dinucleotide binding / iron ion binding / heme binding / identical protein binding / cytosol Similarity search - Function
Mass: 18.015 Da / Num. of mol.: 869 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION
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Sample preparation
Crystal
Density Matthews: 2.73 Å3/Da / Density % sol: 55.01 %
Crystal grow
Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 100-160 mM MgCl2, 100 mM MES (pH 6.5) 10-18% PEG 3350 and 5-10 mM Co(III)Sep