Mass: 18.015 Da / Num. of mol.: 181 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.57 Å3/Da / Density % sol: 52.15 % / Description: rod with hexagonal cross-section
Crystal grow
Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: crystallization drop contained 2 ul of purified RstA1-130 (in 20 mM Tris-HCl pH 8.0, 200 mM NaCl, and 1 mM DTT) at 11.25 mg/ml plus 2 ul of reservoir solution against 1 ml of reservoir; ...Details: crystallization drop contained 2 ul of purified RstA1-130 (in 20 mM Tris-HCl pH 8.0, 200 mM NaCl, and 1 mM DTT) at 11.25 mg/ml plus 2 ul of reservoir solution against 1 ml of reservoir; crystallization cocktail: 20%(v/v) PEG 400, 20%(v/v/) glycerol, 100 mM Tris-HCl, pH 8.5, and 150 mM NaCl