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- PDB-5dzr: Structure of RNA Helicase FRH a Critical Component of the Neurosp... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5dzr | ||||||
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Title | Structure of RNA Helicase FRH a Critical Component of the Neurospora Crassa Circadian Clock | ||||||
![]() | FRQ-interacting RNA helicase | ||||||
![]() | HYDROLASE / helicase / circadian clock / RNA BINDING PROTEIN | ||||||
Function / homology | ![]() RNA catabolic process / maturation of 5.8S rRNA / RNA helicase activity / hydrolase activity / RNA binding / ATP binding / nucleus Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Conrad, K.S. / Crane, B.C. | ||||||
![]() | ![]() Title: Structure of the frequency-interacting RNA helicase: a protein interaction hub for the circadian clock. Authors: Conrad, K.S. / Hurley, J.M. / Widom, J. / Ringelberg, C.S. / Loros, J.J. / Dunlap, J.C. / Crane, B.R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 204.4 KB | Display | ![]() |
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PDB format | ![]() | 158.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 430.8 KB | Display | ![]() |
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Full document | ![]() | 445.4 KB | Display | |
Data in XML | ![]() | 34.4 KB | Display | |
Data in CIF | ![]() | 45.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4xgtSC ![]() 5e02C S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 112830.938 Da / Num. of mol.: 1 / Fragment: residues 114-1106 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987 Gene: frh, NCU03363 / Production host: ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.31 Å3/Da / Density % sol: 46.66 % |
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Crystal grow | Temperature: 290 K / Method: vapor diffusion, hanging drop / pH: 5.5 / Details: sodium citrate, PEG600 |
-Data collection
Diffraction | Mean temperature: 100 K | |||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||||||||||||||
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Aug 22, 2014 | |||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.987 Å / Relative weight: 1 | |||||||||||||||||||||||||||
Reflection | Resolution: 3.16→125.77 Å / Num. obs: 18063 / % possible obs: 98.1 % / Redundancy: 4 % / Biso Wilson estimate: 92.4 Å2 / CC1/2: 0.996 / Rmerge(I) obs: 0.143 / Rpim(I) all: 0.08 / Net I/σ(I): 9.6 / Num. measured all: 71555 | |||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1 / Rejects: _
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4XGT Resolution: 3.161→81.826 Å / SU ML: 0.61 / Cross valid method: NONE / σ(F): 1.34 / Phase error: 37.58 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 179.7 Å2 / Biso mean: 92.2313 Å2 / Biso min: 46.15 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 3.161→81.826 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 6
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