PDB-5d17: Structure of the C-terminal domain of TnsE at 2.85 resolution

Basic information

• Entry: Database: PDB / ID: 5d17
• Title: Structure of the C-terminal domain of TnsE at 2.85 resolution
• Components: Transposon Tn7 transposition protein TnsE
• Keywords: DNA BINDING PROTEIN / Transposition / Tn7 / DNA binding proteins / conformational toggle
• Function / homology: Transposition, TnsE / TnsE, C-terminal / TnsE C-terminal domain / transposition / DNA recombination / DNA binding / Transposon Tn7 transposition protein TnsE
• Biological species: Escherichia coli (E. coli)
• Method: X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2.85 Å
• Authors: Guarne, A. / Caron, J.J.

Funding support

Canada, 1items

Organization	Grant number	Country
Canadian Institutes of Health Research (CIHR)	MOP-67189	Canada

• Citation: Journal: Nucleic Acids Res. / Year: 2015; Title: Conformational toggling controls target site choice for the heteromeric transposase element Tn7.; Authors: Shi, Q. / Straus, M.R. / Caron, J.J. / Wang, H. / Chung, Y.S. / Guarne, A. / Peters, J.E.

History

Deposition	Aug 3, 2015	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Sep 30, 2015	Provider: repository / Type: Initial release
Revision 1.1	Dec 23, 2015	Group: Database references
Revision 1.2	Sep 27, 2017	Group: Author supporting evidence / Database references / Derived calculations Category: citation / pdbx_audit_support / pdbx_struct_oper_list Item: _citation.journal_id_CSD / _pdbx_audit_support.funding_organization / _pdbx_struct_oper_list.symmetry_operation
Revision 1.3	Jan 8, 2020	Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization

Assembly

Deposited unit

A: Transposon Tn7 transposition protein TnsE

B: Transposon Tn7 transposition protein TnsE

C: Transposon Tn7 transposition protein TnsE

D: Transposon Tn7 transposition protein TnsE

E: Transposon Tn7 transposition protein TnsE

F: Transposon Tn7 transposition protein TnsE

G: Transposon Tn7 transposition protein TnsE

H: Transposon Tn7 transposition protein TnsE

I: Transposon Tn7 transposition protein TnsE

K: Transposon Tn7 transposition protein TnsE

L: Transposon Tn7 transposition protein TnsE

M: Transposon Tn7 transposition protein TnsE

Summary:

• 279 kDa, 12 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	278,791	12
Polymers	278,791	12
Non-polymers	0	0
Water	414	23

1

A: Transposon Tn7 transposition protein TnsE

Summary:

• defined by author&software
• 23.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	23,233	1
Polymers	23,233	1
Non-polymers	0	0
Water	18	1

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Method	PISA

2

B: Transposon Tn7 transposition protein TnsE

Summary:

• defined by author&software
• 23.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	23,233	1
Polymers	23,233	1
Non-polymers	0	0
Water	18	1

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Method	PISA

3

C: Transposon Tn7 transposition protein TnsE

Summary:

• defined by author&software
• 23.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	23,233	1
Polymers	23,233	1
Non-polymers	0	0
Water	18	1

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Method	PISA

4

D: Transposon Tn7 transposition protein TnsE

Summary:

• defined by author&software
• 23.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	23,233	1
Polymers	23,233	1
Non-polymers	0	0
Water	18	1

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Method	PISA

5

E: Transposon Tn7 transposition protein TnsE

Summary:

• defined by author&software
• 23.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	23,233	1
Polymers	23,233	1
Non-polymers	0	0
Water	18	1

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Method	PISA

6

F: Transposon Tn7 transposition protein TnsE

Summary:

• defined by author&software
• 23.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	23,233	1
Polymers	23,233	1
Non-polymers	0	0
Water	18	1

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Method	PISA

7

G: Transposon Tn7 transposition protein TnsE

Summary:

• defined by author&software
• 23.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	23,233	1
Polymers	23,233	1
Non-polymers	0	0
Water	18	1

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Method	PISA

8

H: Transposon Tn7 transposition protein TnsE

Summary:

• defined by author&software
• 23.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	23,233	1
Polymers	23,233	1
Non-polymers	0	0
Water	18	1

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Method	PISA

9

I: Transposon Tn7 transposition protein TnsE

Summary:

• defined by author&software
• 23.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	23,233	1
Polymers	23,233	1
Non-polymers	0	0
Water	18	1

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Method	PISA

10

K: Transposon Tn7 transposition protein TnsE

Summary:

• defined by author&software
• 23.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	23,233	1
Polymers	23,233	1
Non-polymers	0	0
Water	18	1

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Method	PISA

11

L: Transposon Tn7 transposition protein TnsE

Summary:

• defined by author&software
• 23.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	23,233	1
Polymers	23,233	1
Non-polymers	0	0
Water	18	1

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Method	PISA

12

M: Transposon Tn7 transposition protein TnsE

Summary:

• defined by author&software
• 23.2 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	23,233	1
Polymers	23,233	1
Non-polymers	0	0
Water	18	1

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Method	PISA

Unit cell

Length a, b, c (Å)	110.330, 87.125, 141.621
Angle α, β, γ (deg.)	90.00, 95.01, 90.00
Int Tables number	4
Space group name H-M	P1211

Components

#1: Protein

A B C D E F G H I K L M
Transposon Tn7 transposition protein TnsE / Protein D

Details:

Mass: 23232.611 Da / Num. of mol.: 12 / Fragment: C-terminal domain (UNP residues 342-538)
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: tnsE / Plasmid: pET22b / Production host: Escherichia coli (E. coli) / Strain (production host): B834 (DE3) / References: UniProt: P05845

#2: Water

ChemComp-HOH / water / Water

Details:

Mass: 18.015 Da / Num. of mol.: 23 / Source method: isolated from a natural source / Formula: H₂O

Experimental details

Experiment

• Experiment: Method: X-RAY DIFFRACTION / Number of used crystals: 1

Sample preparation

• Crystal: Density Matthews: 2.43 ų/Da / Density % sol: 49.43 %
• Crystal grow: Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 8 / Details: PEG 3350, NaCl, TRIS

Data collection

• Diffraction: Mean temperature: 100 K
• Diffraction source: Source: SYNCHROTRON / Site: NSLS / Beamline: X25 / Wavelength: 0.979 Å
• Detector: Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jun 6, 2014
• Radiation: Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
• Radiation wavelength: Wavelength: 0.979 Å / Relative weight: 1
• Reflection: Resolution: 2.85→43.2 Å / Num. obs: 122729 / % possible obs: 100 % / Redundancy: 10.6 % / R_merge(I) obs: 0.114 / Net I/σ(I): 21
• Reflection shell: Resolution: 2.85→2.9 Å / Redundancy: 10.1 % / R_merge(I) obs: 1 / Mean I/σ(I) obs: 1.9 / % possible all: 100

Processing

Software

Name	Version	Classification
PHENIX	1.9_1692	refinement
HKL-2000		data reduction
HKL-2000		data scaling
PHENIX		phasing

Refinement

Method to determine structure: SAD / Resolution: 2.85→39.749 Å / SU ML: 0.42 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 29.3 / Stereochemistry target values: ML

	Rfactor	Num. reflection	% reflection	Selection details
Rfree	0.2505	3707	3.02 %	random selection
Rwork	0.2205	-	-	-
obs	0.2214	122611	99.91 %	-

• Solvent computation: Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Refinement step

Cycle: LAST / Resolution: 2.85→39.749 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	14406	0	0	23	14429

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.003	15189
X-RAY DIFFRACTION	f_angle_d	0.756	20593
X-RAY DIFFRACTION	f_dihedral_angle_d	12.527	5397
X-RAY DIFFRACTION	f_chiral_restr	0.03	2360
X-RAY DIFFRACTION	f_plane_restr	0.003	2672

LS refinement shell

Resolution (Å)	Rfactor Rfree	Num. reflection Rfree	Rfactor Rwork	Num. reflection Rwork	Refine-ID	% reflection obs (%)
2.85-2.8875	0.3555	143	0.37	4592	X-RAY DIFFRACTION	100
2.8875-2.927	0.4073	145	0.3512	4638	X-RAY DIFFRACTION	100
2.927-2.9688	0.3474	143	0.3462	4568	X-RAY DIFFRACTION	100
2.9688-3.0131	0.4024	142	0.3376	4563	X-RAY DIFFRACTION	100
3.0131-3.0602	0.3375	145	0.3199	4571	X-RAY DIFFRACTION	100
3.0602-3.1104	0.4069	142	0.3197	4578	X-RAY DIFFRACTION	100
3.1104-3.164	0.3104	146	0.2965	4550	X-RAY DIFFRACTION	100
3.164-3.2215	0.2596	143	0.2931	4635	X-RAY DIFFRACTION	100
3.2215-3.2834	0.3497	139	0.3006	4506	X-RAY DIFFRACTION	100
3.2834-3.3504	0.3156	147	0.3013	4641	X-RAY DIFFRACTION	100
3.3504-3.4232	0.2774	139	0.2748	4483	X-RAY DIFFRACTION	100
3.4232-3.5028	0.3554	139	0.2755	4636	X-RAY DIFFRACTION	100
3.5028-3.5903	0.3291	143	0.262	4556	X-RAY DIFFRACTION	100
3.5903-3.6873	0.2748	144	0.2383	4588	X-RAY DIFFRACTION	100
3.6873-3.7958	0.2914	145	0.2364	4555	X-RAY DIFFRACTION	100
3.7958-3.9182	0.2766	141	0.219	4543	X-RAY DIFFRACTION	100
3.9182-4.0581	0.2252	146	0.2095	4602	X-RAY DIFFRACTION	100
4.0581-4.2204	0.2183	142	0.1944	4553	X-RAY DIFFRACTION	100
4.2204-4.4122	0.2281	144	0.1944	4585	X-RAY DIFFRACTION	100
4.4122-4.6445	0.1934	139	0.1847	4565	X-RAY DIFFRACTION	100
4.6445-4.935	0.1695	145	0.1733	4581	X-RAY DIFFRACTION	100
4.935-5.3153	0.2026	137	0.1703	4548	X-RAY DIFFRACTION	100
5.3153-5.8486	0.2337	146	0.1854	4616	X-RAY DIFFRACTION	100
5.8486-6.6915	0.2799	137	0.2119	4576	X-RAY DIFFRACTION	100
6.6915-8.4174	0.2101	143	0.1996	4545	X-RAY DIFFRACTION	100
8.4174-39.7528	0.1891	142	0.1633	4530	X-RAY DIFFRACTION	99

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°2)	L12 (°2)	L13 (°2)	L22 (°2)	L23 (°2)	L33 (°2)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å2)	T12 (Å2)	T13 (Å2)	T22 (Å2)	T23 (Å2)	T33 (Å2)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	2.1703	-1.7764	1.0963	8.5335	4.3884	4.4584	0.399	-0.3097	0.0998	-0.5917	0.5328	0.0964	-0.0015	-0.5014	-0.7584	0.7666	-0.2367	-0.0637	0.7269	-0.0296	0.8841	0.6179	35.978	73.3906
2	5.8975	-1.0784	-0.3953	6.3293	0.7178	3.9006	0.0143	0.3992	0.0051	-0.9576	0.2412	0.2499	-1.038	-0.2074	-0.2538	0.6137	-0.2193	0.0269	0.4012	-0.0966	0.578	1.6102	30.9962	68.7272
3	8.1547	-1.0492	-5.5706	7.5317	4.8027	6.0543	0.8543	-0.816	0.7791	1.2079	-0.2615	0.7626	-0.3439	-0.0263	0.2727	0.6981	-0.0969	-0.0627	0.5675	-0.0144	0.5628	13.456	27.223	70.9944
4	1.0826	-1.3971	0.1741	1.9927	-0.0851	2.0201	0.4951	0.0247	-0.6149	-0.1659	-0.2762	1.1759	1.0754	-0.174	0.1944	0.7166	-0.3872	-0.0727	0.6167	-0.0397	0.7444	-2.9898	22.6677	66.5083
5	5.2223	0.08	0.1739	2.7308	-5.0067	9.5055	0.4178	-0.677	-0.425	-1.0511	-0.4164	-0.663	0.8849	0.7713	-0.5267	0.6668	-0.2582	0.1932	0.5459	-0.1597	0.7743	7.4578	19.1619	72.8552
6	2.5352	-1.1997	-0.691	3.718	-1.5526	2.6923	-0.4742	0.1697	-0.355	-1.2515	1.2519	-0.3533	0.5876	0.8145	-0.5198	0.866	-0.3515	0.1175	0.932	-0.2461	1.0071	5.104	20.6715	61.4946
7	4.4036	1.0207	-2.425	5.8631	1.9485	4.4282	-1.2112	0.3606	-0.0454	-1.0528	0.4916	-0.4011	-0.6922	1.0314	2.4113	1.2475	-0.3339	-0.1537	1.1665	-0.2687	1.0148	18.7321	39.8444	66.0263
8	5.399	1.9354	2.1211	1.6289	0.7167	2.2137	0.3556	1.4319	-0.0932	-0.5064	-0.9568	-0.359	0.3969	0.8222	0.4887	0.6153	0.2662	0.1136	1.0988	0.0218	0.8353	37.2035	23.8508	74.7075
9	3.5038	-1.3923	2.9718	7.0509	-1.4241	2.4987	-0.4607	2.0375	-0.7141	-2.2045	0.2163	0.0306	0.45	0.1083	0.7376	0.8004	0.1818	0.1903	2.3528	-0.0294	0.662	38.33	26.7486	57.1154
10	2.8215	-0.682	0.7392	5.715	0.5033	3.609	0.2019	0.8307	-0.2508	-0.576	-0.4391	-0.547	0.3038	-0.1101	0.1186	0.4402	0.2296	0.1138	0.8467	0.1091	0.6638	42.4453	28.3451	75.9127
11	4.3732	4.3351	-0.2274	4.5849	0.6026	2.7363	-0.4437	0.1258	-1.0614	-1.6275	0.1563	-0.4783	1.246	0.1717	0.0183	0.9671	0.2203	0.0236	0.7669	0.0282	0.6765	40.096	39.3185	75.102
12	1.9653	-1.3446	0.8177	6.4531	-0.1629	0.474	-0.0359	-0.0973	0.1719	0.362	0.0733	-2.4036	-0.1968	-0.398	-0.2086	0.7189	0.322	0.2931	0.9902	0.1771	0.9206	53.2723	25.3998	73.3154
13	1.8128	-0.3255	-0.6728	0.8502	0.1853	0.2597	-0.4827	-1.0916	-0.0235	1.0794	0.8416	-0.859	1.0543	0.0243	-0.1086	0.624	0.211	0.0362	0.8983	0.1161	1.0353	51.6876	27.649	83.4184
14	3.8856	0.7222	-1.0764	2.7331	0.3953	3.3841	0.3412	1.1267	0.839	-0.5067	-0.451	-1.0873	-1.1359	-0.0502	0.4168	0.7049	0.3484	0.2358	0.93	0.2623	1.0903	49.89	36.7158	74.8956
15	0.4512	1.4771	0.5353	6.3683	0.8605	1.5155	0.5052	0.1984	0.3919	-1.3374	-1.1866	0.718	-0.6718	-2.147	-0.3981	1.2067	0.7973	0.2128	2.4793	0.2504	0.8579	36.1165	37.9313	61.3947
16	6.6034	-4.8494	-0.0271	3.9706	-0.6813	2.9385	-0.4249	-0.1983	1.8641	0.1978	0.5409	0.3269	-1.3923	1.4627	-0.7497	0.7176	-0.143	0.045	0.4446	-0.0887	1.2535	27.5416	60.7082	83.7147
17	4.9049	2.2405	-0.908	4.1746	0.3114	6.4333	-0.2152	0.5503	1.0571	0.0088	0.332	-0.0699	-1.3495	0.607	-0.1682	0.6927	-0.0848	-0.0981	0.5285	0.0828	0.9719	24.5297	62.6758	76.4885
18	7.1966	3.695	0.205	5.8047	1.5607	4.7231	0.3024	-0.1375	-0.5916	0.0932	0.0135	-0.8635	1.3437	0.9985	-0.1783	0.8942	0.1421	0.0063	0.7168	0.0427	1.0883	17.4741	53.6268	90.437
19	8.0772	1.0841	-2.8194	2.8047	-1.6657	8.8134	-0.1503	0.9465	0.8993	-0.3518	0.6092	0.0466	-1.2214	0.6309	-0.5372	0.7801	-0.2613	-0.036	0.5533	-0.0322	0.9722	22.607	63.0337	75.7106
20	0.1054	0.6717	0.4856	7.8148	5.403	3.7518	-0.2293	1.0019	1.3728	-0.9832	-0.0845	-1.1242	-1.4447	1.0126	0.4976	0.6782	-0.0302	-0.0423	0.6747	0.3194	0.9149	14.3073	55.6774	74.9811
21	2.4957	0.6324	-1.7116	1.1654	-0.9796	3.5823	0.2845	1.0115	3.1212	0.1469	0.8702	0.8022	-2.6685	-1.1718	0.4067	1.2412	0.035	0.1969	0.2751	0.3296	1.8963	14.7192	70.2748	76.3586
22	2.9255	0.6656	5.5288	0.2546	1.3481	1.9827	0.3702	0.2332	-0.4375	0.2092	0.2959	-0.6001	1.1747	-0.0871	-0.5622	0.5313	-0.2585	0.0923	0.7964	-0.0785	1.2486	17.7492	50.3404	69.4403
23	6.5407	-1.6251	-2.9716	0.4702	0.7395	1.5343	0.203	1.0907	-0.4093	-1.8133	-0.436	0.8033	0.7705	0.2443	0.4059	0.9109	-0.3389	-0.015	1.1115	0.0526	0.9729	22.7347	52.5187	64.1325
24	4.9881	1.0371	1.2934	2.9144	1.5278	2.4703	-0.2383	-0.1237	-0.8077	0.5398	0.7914	0.2243	1.1037	-1.0459	-0.551	0.928	-0.0449	0.1693	0.6581	-0.048	0.8632	-1.7249	36.7306	91.3862
25	8.5105	0.3829	-2.0055	5.3937	-0.9006	5.5645	-0.5337	-0.5547	-0.4561	0.7997	0.4496	0.2386	0.8875	-0.7419	0.034	0.6988	0.0742	0.0292	0.5146	0.0484	0.6789	-2.9842	42.4543	95.4563
26	6.3874	1.0442	4.6353	8.2417	1.9787	4.4767	-0.7298	0.5586	-1.4862	-0.0981	-0.3597	0.5578	1.0476	0.5576	-0.2236	0.9683	0.1281	0.2562	0.5996	0.1253	0.5581	7.8496	48.0436	96.3141
27	1.0967	1.0399	0.2359	1.5712	0.2958	0.2887	0.4575	-0.5696	0.4943	0.7587	0.6104	1.7714	-0.7539	-2.2867	-0.5696	0.793	0.3258	0.2221	1.1562	0.2801	0.8719	-9.9995	49.6858	96.1248
28	5.6242	-1.895	-0.542	5.9445	-0.279	2.1406	-0.2519	-0.375	1.9238	1.8828	1.2382	0.4953	-1.8128	-0.387	-1.2982	0.8176	0.2192	0.0852	0.5134	-0.001	0.8176	1.4117	54.9506	92.8302
29	3.8071	2.0377	-0.3221	6.3444	-1.0148	5.5684	0.5928	-0.0728	1.4895	1.5757	0.0031	0.7765	-1.0307	-0.616	-0.3554	0.9702	0.2692	0.1594	0.8354	-0.0114	1.0159	-3.3155	52.6833	102.9855
30	6.0172	4.0915	-1.3815	3.7861	2.2574	1.9909	1.669	0.1973	-1.5162	0.0997	0.1722	-0.8758	-1.5669	2.2796	-0.3613	1.7071	0.7507	0.1441	1.3665	0.2115	1.2725	13.1279	36.5585	103.5338
31	3.9315	0.7234	2.7865	9.1906	-3.6784	4.4473	0.4715	-1.4734	-0.15	1.4344	0.1086	-0.4807	1.5276	2.1709	-0.0636	1.3363	0.1441	-0.0178	1.1155	0.1039	0.4949	3.4015	39.9613	109.8903
32	4.8728	0.6266	0.5695	4.9191	0.6105	2.1821	0.2139	-0.2377	-1.0419	-0.033	0.2239	-0.1776	1.5029	1.1551	-0.607	0.8242	0.1672	-0.0625	0.7289	0.0682	0.884	31.1293	16.5453	88.6036
33	9.6682	-2.3901	0.1312	3.6422	1.2916	4.5567	-0.6744	-0.539	-0.0109	2.2402	0.3494	-0.328	-0.2481	0.9628	0.5245	1.0705	0.2056	0.0476	0.8576	0.334	0.9781	29.9528	12.1247	106.0997
34	6.0506	-0.6304	2.301	4.4191	-0.3166	7.3873	0.0528	0.1931	-0.9555	0.358	-0.3114	-0.3393	0.4363	0.6263	0.0676	0.5147	0.0777	0.0388	0.3771	-0.0489	0.8724	24.2173	12.895	88.9165
35	8.4352	6.4351	3.2101	9.2651	-3.1811	8.5281	-0.8975	0.1906	0.3984	0.2487	-0.2486	-1.5375	-1.7935	0.8804	0.3386	0.7505	0.1604	-0.0353	0.4624	0.2019	0.8266	15.3036	20.0537	91.9539
36	4.9617	-2.323	1.2071	1.168	-0.6452	4.1984	0.1688	-0.308	-2.1884	0.1032	0.6414	0.5053	1.1113	-0.1983	-0.5168	1.0147	0.0379	-0.0333	0.5757	0.0379	1.5155	17.822	4.7177	90.4336
37	5.9565	1.4471	1.8882	5.8038	2.3585	1.3011	0.8963	0.2539	-0.309	0.3391	-1.4188	0.1997	0.0526	0.3961	0.3308	1.1442	0.1983	0.3909	0.8559	0.4008	1.2607	16.8552	22.845	100.8902
38	2.6387	-1.8349	4.0957	8.9918	-2.9352	9.5379	-1.449	1.4866	1.6322	1.0557	-0.4537	1.2573	-0.4161	-0.5559	1.1863	1.6165	-0.0003	0.2757	0.9717	0.0297	1.5434	20.1323	27.4353	105.1859
39	8.8007	2.8419	4.3379	5.0714	3.5269	3.188	-0.1057	-1.3829	1.643	0.785	-1.034	0.5689	-0.3081	0.1183	0.9909	1.3504	0.4346	0.4183	1.2501	0.2919	1.0805	20.216	15.4847	107.9209
40	3.7364	0.5029	-0.5249	2.8656	-0.1359	7.4346	0.2354	-0.3557	0.3947	0.4123	0.091	-0.603	-0.8463	0.3897	-0.0941	0.8419	0.0018	-0.0892	0.9056	-0.1488	0.9671	30.0192	55.5454	98.2771
41	4.4062	-0.0951	-0.7	5.1906	0.3322	5.0329	0.16	-1.0695	0.6681	0.6175	0.1895	-0.342	-0.7641	0.2403	-0.2593	0.644	-0.0019	-0.0893	0.8502	-0.0222	0.8298	34.8992	52.0869	101.1402
42	4.066	-0.6741	-2.1072	3.6885	-1.0529	2.4361	0.2448	-0.765	0.2287	0.7523	-0.1957	-0.0023	-0.823	-0.1562	-0.2261	0.7577	-0.0136	-0.1356	0.7899	-0.1758	1.0432	34.8308	51.0293	103.5628
43	1.5096	-0.3489	-2.0416	3.1017	-1.9196	4.6429	-0.7373	-1.6322	1.7988	1.6652	0.5089	-0.957	-1.5093	0.1208	0.3257	0.7798	0.0699	-0.0001	1.4098	-0.4016	0.8587	35.2075	40.3749	101.227
44	3.6429	-0.1393	-1.0342	3.2683	-0.785	4.2264	0.0478	-0.7005	0.5952	1.0878	-0.1329	-1.2657	-0.4255	1.1931	0.0742	0.8956	-0.1907	-0.2701	1.1451	-0.0684	0.9986	46.4781	50.7723	103.6158
45	7.3511	-0.0468	-3.0303	5.3002	3.4687	9.5515	-0.3396	-1.5742	-0.1349	0.9129	1.0508	-0.9862	0.0804	0.3807	-0.5861	0.8873	-0.0162	-0.1984	1.5937	0.1622	1.0946	40.5352	42.9692	110.9491
46	1.6091	2.5601	0.4024	4.0771	0.6605	0.1048	0.183	-0.2365	-2.0494	-1.9768	0.3995	0.2803	0.8284	-1.2821	0.1675	1.4109	-0.0801	-0.6278	1.3895	0.0707	1.3396	27.1716	38.419	103.8398
47	5.7571	-1.7748	-0.7777	5.8863	-2.3882	4.1645	0.9002	-1.0936	-1.342	0.3439	0.2153	1.0636	1.2261	-2.4943	-1.8304	1.1372	-0.3495	-0.1762	2.3959	0.3733	1.0359	26.5544	41.8659	112.9881
48	2.2152	-0.7109	0.9876	1.6853	1.7183	3.5097	-0.0018	-0.2001	-0.0573	0.1304	0.1497	-0.3788	-0.4437	-0.0309	0.0436	0.6786	-0.1635	-0.0344	0.4817	-0.1138	0.8398	-5.5411	29.3198	140.7939
49	5.9176	-1.4446	-2.3316	6.0823	2.2741	5.4963	-0.1723	1.303	0.2516	-1.5344	0.5081	0.6335	-1.5063	-0.2095	-0.6245	1.1577	-0.0945	-0.1192	0.9491	0.2106	0.5495	-6.1224	33.4845	123.8207
50	5.9436	-0.0827	-0.1843	4.7623	-0.8009	2.9184	-0.1017	0.1524	-0.4691	0.0597	0.2803	0.0744	-0.3111	0.1396	-0.2928	0.5098	-0.1322	0.0226	0.3653	-0.1846	0.7248	-5.3265	20.7613	143.9305
51	5.4582	-1.7819	-0.8309	3.6576	-0.5198	1.0998	0.1927	1.0674	0.0111	-0.1999	-0.0637	-0.0102	-0.0584	0.2962	0.3002	0.605	-0.2234	0.085	0.568	-0.1209	0.7046	-3.5275	20.0482	134.4859
52	5.8247	-7.2528	-2.5622	9.106	3.2436	1.2568	1.0052	0.216	-2.4244	0.2373	-0.7117	-0.2046	1.0811	-0.4761	0.489	0.9433	-0.0855	0.1222	0.7086	-0.2077	0.8627	-13.9054	13.2118	139.919
53	1.8692	0.5307	1.5761	1.0078	1.3438	6.5653	0.0385	0.6506	-0.7239	0.0696	0.4553	-0.6973	0.9417	0.0782	-0.7808	0.6025	-0.1887	0.045	0.682	-0.2847	0.9628	-4.5047	10.0086	137.6043
54	2.5038	-1.5029	-2.681	5.7573	0.4002	4.9457	-0.8482	0.6962	0.0731	-1.12	0.3101	-0.4045	-0.2407	-0.1284	-0.3305	0.7282	-0.3231	0.1651	0.9909	-0.2581	1.1869	-0.6144	15.3597	126.8364
55	7.7357	0.157	2.5566	6.5633	2.758	5.1873	-0.836	0.3971	-1.102	-0.1173	0.6584	0.1893	-0.2184	0.4174	0.2193	0.5248	-0.2104	0.0546	1.0598	-0.3504	0.9557	11.1593	28.3277	135.9621
56	3.1654	-0.0603	2.3267	4.2625	-2.4426	3.6725	-0.4995	1.615	0.9808	-0.4926	0.0321	0.3459	-0.3899	1.3956	0.7617	1.1317	-0.4592	0.0698	1.2966	0.0192	0.7478	5.0345	29.4	126.5449
57	4.2875	3.2828	0.9505	5.0492	-1.8088	3.0515	0.025	2.0088	-1.2257	-1.0422	-1.038	0.4451	1.9402	0.9552	0.3644	0.7777	0.2783	0.0075	1.3054	-0.4157	1.0696	29.2609	16.1267	143.9601
58	2.4867	0.008	0.6651	4.2953	-0.8882	1.423	0.1381	1.2327	-0.4622	-0.5432	-0.2094	0.0319	0.4757	-0.0881	0.0359	0.5323	0.2347	-0.0746	1.1487	-0.3718	0.8	35.3974	20.0201	143.7294
59	4.2074	3.8691	-0.6524	4.4382	0.5813	2.5651	-0.2126	1.06	-0.4415	-0.9319	0.3557	-0.0664	1.1182	0.1415	0.1166	0.8002	0.1865	0.0994	0.977	-0.2233	0.5802	33.9871	31.0078	144.9943
60	1.551	0.722	0.9074	3.5128	-1.0198	1.5484	0.9986	0.2466	-1.4313	-0.0993	-0.4021	-1.1243	1.0592	0.637	-0.1495	-0.0595	1.1342	0.5519	0.7954	-1.0601	0.2841	45.9067	17.996	145.9526
61	1.9745	0.364	0.1819	6.1871	-1.1007	5.093	0.1081	1.404	-0.4359	0.1529	0.4402	-0.7731	-1.2771	0.6292	-0.5639	0.5171	0.0806	0.0057	0.736	-0.1713	0.5362	40.3339	28.5923	152.582
62	2.4398	0.4948	0.7543	4.862	1.0778	2.9592	-0.0575	0.3293	0.4822	-0.1388	0.4293	-0.8561	-0.4679	0.7383	-0.4281	0.6766	0.2131	0.1978	1.1517	-0.0637	0.7349	45.1054	28.209	141.8608
63	5.9817	-1.4482	1.1542	3.6068	0.6642	2.6542	0.7096	0.7432	-1.0553	0.0071	-0.8392	1.0897	0.5075	-0.479	0.2278	1.0038	0.4196	-0.25	1.636	0.0291	1.0453	22.652	32.079	134.3398
64	4.5085	0.8671	-1.4309	9.182	-6.3838	4.6405	1.431	0.4557	0.7127	-1.4209	-1.2325	1.2334	-1.0425	-0.6314	-1.1091	1.2876	0.4254	0.1406	1.9407	-0.1757	0.6701	33.7383	28.9256	129.8936
65	8.7407	-2.6034	-0.5325	2.0799	-1.2589	3.8382	-0.2675	0.1082	1.241	0.0238	0.4143	-0.0695	-0.8361	0.5429	-0.2785	0.5587	-0.1328	0.0276	0.3598	0.0833	0.8985	21.9256	51.9353	153.8903
66	4.7309	0.3207	1.3061	3.5367	2.398	3.4894	-0.4892	0.6034	1.0469	-0.2134	0.2799	-0.293	-0.53	0.9821	0.0296	0.5292	-0.1174	-0.023	0.3175	0.2206	0.9103	20.3972	55.1638	145.5197
67	3.3637	2.8908	1.2023	6.1644	1.6378	3.6807	0.2608	0.4885	-0.1705	0.6341	0.0353	-0.576	1.0202	0.3289	-0.2572	0.5704	0.0518	-0.0255	0.4104	0.0549	0.5951	8.1838	47.1623	160.9907
68	8.3322	-0.3579	-1.141	3.495	0.3856	8.4237	-0.0963	0.8963	0.4883	-0.4701	0.3766	-0.337	-0.245	0.1316	-0.3819	0.3839	-0.044	0.0192	0.2954	0.0188	0.6721	16.9021	54.0838	147.0929
69	2.265	2.071	2.16	2.6054	2.8353	3.0979	-0.0384	0.9005	1.8829	-1.396	-0.4764	-1.0458	-0.7005	1.0471	-0.2329	0.5816	0.0223	0.2743	0.6022	0.3198	0.6619	8.4943	47.6622	145.5994
70	3.234	-0.0562	-1.7898	3.0514	-1.0034	4.1635	0.212	0.6207	2.0809	0.1137	0.6912	0.3406	-1.7419	-0.4579	-0.3803	0.9821	0.0286	0.1529	0.5052	0.2287	1.2737	9.2694	62.2334	147.4568
71	4.2082	-5.036	4.9311	6.3142	-5.4304	6.4428	0.8054	0.2406	-0.7574	-0.7583	0.2554	0.8915	1.7707	-0.8905	-1.1408	0.4517	-0.1572	0.0757	0.7073	-0.031	0.7552	11.6446	42.4124	139.8916
72	6.5619	-2.5027	-2.2715	6.7532	0.7326	6.3079	-0.1924	1.5928	0.2899	-1.407	0.2748	-0.1907	0.4439	0.8872	-0.102	0.6516	-0.3132	0.0249	0.9781	0.0474	0.6925	16.8393	45.2712	134.5271
73	5.0157	1.7519	-1.4857	2.3377	1.3761	5.9038	-0.4463	0.0245	-0.267	1.9003	-0.0334	0.0299	0.6603	-0.8753	0.5064	1.0803	0.0039	0.0738	0.4354	-0.1163	0.9473	-8.0053	28.7511	160.531
74	6.0681	-0.8508	0.9696	5.4396	-0.9782	4.9471	-0.0123	-0.1676	-0.2365	1.3059	0.1404	0.1675	0.9617	-0.6732	-0.2268	0.9325	-0.0891	0.0577	0.2976	-0.0713	0.5127	-8.3147	34.1697	164.4471
75	3.7801	1.1451	0.3048	5.2716	-2.103	9.3634	0.1497	-0.3159	-0.8077	1.4511	0.0673	0.1021	1.135	-0.665	-0.1719	1.0438	-0.0743	0.0449	0.3098	0.1217	0.6463	-8.3931	34.2302	166.7667
76	8.4626	0.162	5.9448	2.0919	-0.716	4.5125	-0.3841	0.2493	-0.3322	0.5245	0.047	-0.9893	0.9268	1.0664	0.1641	1.0546	0.1033	0.0961	0.4156	0.0199	0.6282	1.1494	39.7162	168.3662
77	8.6819	-1.9869	3.5299	7.9484	4.0394	5.3985	0.8606	-0.7523	0.5752	0.9497	-1.0172	2.2774	0.119	-2.5083	0.5343	0.7115	-0.0895	0.3677	0.8685	0.1529	0.9599	-18.0712	40.4175	168.396
78	5.1104	0.5251	-1.0621	4.0186	0.5227	3.9131	1.1399	1.8646	1.998	0.2698	0.1949	0.2235	-1.2614	-0.5227	-0.6238	0.4729	0.0962	0.1229	0.3759	0.11	0.5092	-11.0488	46.3783	160.7425
79	3.6844	2.264	-0.1265	4.6445	1.663	5.7387	0.3318	-0.3953	0.2399	1.3332	-0.0092	0.495	-0.3397	-0.0782	-0.2864	0.9742	0.0583	0.0982	0.42	0.0627	0.5876	-7.4608	44.2373	172.0939
80	4.3322	2.1844	0.7817	3.6824	-0.9409	3.3196	0.5779	-0.6508	-1.2915	1.7353	0.325	-0.8143	-0.2586	0.6481	-0.0257	1.8386	0.0551	-0.1683	0.8885	0.1732	0.8255	1.364	29.9661	177.7273
81	6.3433	2.7677	0.4884	4.364	-2.3799	5.7807	0.6071	-0.058	-1.1557	0.3343	0.0347	-1.2596	1.1413	0.6799	-0.4942	0.7324	0.0109	-0.3072	0.5741	-0.0928	1.2758	23.9353	8.7555	158.212
82	4.6611	-2.9821	-4.3914	2.6565	3.3317	4.4982	-0.1135	-1.8966	-0.4398	0.7995	0.2742	-0.5558	0.6331	2.3878	-1.211	1.4285	-0.3804	-0.4118	2.0781	0.3955	1.2902	21.3329	6.5358	176.5128
83	6.9616	-1.3441	0.9804	3.3024	0.7148	7.3168	0.2154	-0.6884	-0.9455	0.2778	-0.081	-0.2315	0.5671	0.5581	0.0861	0.6744	-0.0477	-0.1773	0.584	-0.1587	1.2352	17.4339	4.9595	159.1278
84	5.8485	4.4477	-0.1338	4.7293	-3.9467	1.9966	0.2799	-0.2157	0.8044	-1.2207	0.2218	-1.3529	-2.3776	0.8491	0.2823	0.8993	0.0614	-0.0654	0.342	-0.0688	0.7225	7.9595	12.2785	161.3389
85	5.1108	-0.6021	0.3861	1.1473	-1.922	3.1485	0.4446	-1.1564	-3.2855	-0.4259	0.3086	1.0754	1.4417	0.0744	-0.3778	1.1753	-0.0006	-0.2448	0.5133	-0.0009	1.9896	11.3841	-3.077	158.4271
86	8.143	1.9447	-2.3621	2.3087	0.4509	6.6219	0.7748	-2.2139	-1.032	-0.5518	-0.4589	-0.2047	0.5703	-0.3377	-0.1597	0.9463	-0.0549	-0.2713	0.8082	0.1387	1.371	7.5233	4.3967	169.173
87	2.5216	-1.1266	1.2368	5.5173	-1.1037	9.6768	-0.8613	1.3044	0.0056	-2.3596	0.6252	0.4252	1.1211	0.0674	-0.377	2.01	0.0108	0.185	1.5034	-0.4926	1.3894	11.8422	20.4201	174.3386
88	5.6589	-2.2406	-4.2509	1.6728	2.046	3.7781	-0.4372	-2.2512	0.3298	-0.3993	0.4758	-0.1801	-0.4909	-0.377	-0.0484	1.6542	-0.0668	0.3555	1.9856	0.0156	1.3666	12.5115	7.6124	176.7936
89	3.0827	-1.0251	1.6133	5.1382	0.6178	4.8237	-0.1399	-0.4507	0.4266	0.2899	0.0774	0.3177	-1.1162	-0.3682	0.1729	0.6626	0.0894	0.0046	0.6191	-0.1372	0.7042	23.6307	45.8753	173.0665
90	6.9445	3.7001	-6.4583	2.063	-3.6411	6.5014	-0.3387	-2.5554	-0.9821	-0.1871	-0.7789	-0.2176	-0.5432	1.0383	0.6201	1.0881	0.4235	0.0098	1.1489	0.0342	0.6737	24.1161	44.5299	188.9843
91	5.3633	-0.4664	0.5221	4.4029	0.2194	7.749	-0.2667	-0.4783	0.598	0.5221	0.013	-0.1992	-0.7582	-0.6275	-0.0445	0.4814	0.1374	-0.0842	0.3674	-0.1506	0.5995	31.009	42.2265	169.4185
92	2.1039	0.0985	-3.7639	1.852	0.4805	9.8861	-0.9394	-0.5524	0.8608	1.3824	-0.5407	-0.4281	-0.7059	0.0164	0.2375	0.815	-0.1253	-0.0436	0.6659	-0.1548	0.6802	29.3208	31.56	171.3884
93	4.9304	-0.4755	0.027	4.2337	-0.0898	7.0761	-0.0939	-0.4895	0.5617	0.8721	0.1919	-0.9237	-0.1649	0.9146	-0.0893	0.6039	0.0893	-0.143	0.6192	-0.207	0.6639	40.6914	40.8606	174.574
94	8.8121	2.2096	1.0031	2.4026	-1.0678	9.3747	-0.0019	-1.1104	-2.0927	-1.1013	0.503	-0.3697	1.7109	-1.8049	0.1201	0.7528	0.1945	-0.0152	0.8481	0.0504	0.786	23.4673	29.8602	175.8985
95	9.0778	0.4834	3.6629	3.0359	-2.4428	5.2948	0.3166	-1.2657	-0.359	0.6175	0.7796	2.1585	0.5848	-2.7873	-1.3869	0.9225	-0.0182	0.1367	1.3528	0.1654	0.9107	18.3012	32.4843	182.021
96	4.6096	-0.8654	0.8715	2.0156	0.9591	1.5231	-0.2564	-1.323	-0.4069	0.1153	1.013	-1.0384	0.6123	0.496	-1.4147	1.1279	0.2254	0.0253	1.351	0.0568	0.6213	29.1124	34.3752	188.019

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	( CHAIN A AND RESID 376:395 )	A	376 - 395
2	X-RAY DIFFRACTION	2	( CHAIN A AND RESID 396:456 )	A	396 - 456
3	X-RAY DIFFRACTION	3	( CHAIN A AND RESID 457:460 )	A	457 - 460
4	X-RAY DIFFRACTION	4	( CHAIN A AND RESID 461:484 )	A	461 - 484
5	X-RAY DIFFRACTION	5	( CHAIN A AND RESID 485:491 )	A	485 - 491
6	X-RAY DIFFRACTION	6	( CHAIN A AND RESID 492:514 )	A	492 - 514
7	X-RAY DIFFRACTION	7	( CHAIN A AND RESID 515:520 )	A	515 - 520
8	X-RAY DIFFRACTION	8	( CHAIN B AND RESID 376:397 )	B	376 - 397
9	X-RAY DIFFRACTION	9	( CHAIN B AND RESID 398:402 )	B	398 - 402
10	X-RAY DIFFRACTION	10	( CHAIN B AND RESID 403:457 )	B	403 - 457
11	X-RAY DIFFRACTION	11	( CHAIN B AND RESID 458:461 )	B	458 - 461
12	X-RAY DIFFRACTION	12	( CHAIN B AND RESID 462:477 )	B	462 - 477
13	X-RAY DIFFRACTION	13	( CHAIN B AND RESID 478:482 )	B	478 - 482
14	X-RAY DIFFRACTION	14	( CHAIN B AND RESID 483:514 )	B	483 - 514
15	X-RAY DIFFRACTION	15	( CHAIN B AND RESID 515:533 )	B	515 - 533
16	X-RAY DIFFRACTION	16	( CHAIN C AND RESID 376:392 )	C	376 - 392
17	X-RAY DIFFRACTION	17	( CHAIN C AND RESID 393:423 )	C	393 - 423
18	X-RAY DIFFRACTION	18	( CHAIN C AND RESID 424:428 )	C	424 - 428
19	X-RAY DIFFRACTION	19	( CHAIN C AND RESID 429:457 )	C	429 - 457
20	X-RAY DIFFRACTION	20	( CHAIN C AND RESID 458:461 )	C	458 - 461
21	X-RAY DIFFRACTION	21	( CHAIN C AND RESID 462:509 )	C	462 - 509
22	X-RAY DIFFRACTION	22	( CHAIN C AND RESID 510:515 )	C	510 - 515
23	X-RAY DIFFRACTION	23	( CHAIN C AND RESID 516:533 )	C	516 - 533
24	X-RAY DIFFRACTION	24	( CHAIN D AND RESID 377:395 )	D	377 - 395
25	X-RAY DIFFRACTION	25	( CHAIN D AND RESID 396:456 )	D	396 - 456
26	X-RAY DIFFRACTION	26	( CHAIN D AND RESID 457:460 )	D	457 - 460
27	X-RAY DIFFRACTION	27	( CHAIN D AND RESID 461:484 )	D	461 - 484
28	X-RAY DIFFRACTION	28	( CHAIN D AND RESID 485:491 )	D	485 - 491
29	X-RAY DIFFRACTION	29	( CHAIN D AND RESID 492:514 )	D	492 - 514
30	X-RAY DIFFRACTION	30	( CHAIN D AND RESID 515:521 )	D	515 - 521
31	X-RAY DIFFRACTION	31	( CHAIN D AND RESID 522:533 )	D	522 - 533
32	X-RAY DIFFRACTION	32	( CHAIN E AND RESID 376:392 )	E	376 - 392
33	X-RAY DIFFRACTION	33	( CHAIN E AND RESID 393:407 )	E	393 - 407
34	X-RAY DIFFRACTION	34	( CHAIN E AND RESID 408:456 )	E	408 - 456
35	X-RAY DIFFRACTION	35	( CHAIN E AND RESID 457:460 )	E	457 - 460
36	X-RAY DIFFRACTION	36	( CHAIN E AND RESID 461:509 )	E	461 - 509
37	X-RAY DIFFRACTION	37	( CHAIN E AND RESID 510:515 )	E	510 - 515
38	X-RAY DIFFRACTION	38	( CHAIN E AND RESID 516:521 )	E	516 - 521
39	X-RAY DIFFRACTION	39	( CHAIN E AND RESID 522:533 )	E	522 - 533
40	X-RAY DIFFRACTION	40	( CHAIN F AND RESID 376:394 )	F	376 - 394
41	X-RAY DIFFRACTION	41	( CHAIN F AND RESID 395:427 )	F	395 - 427
42	X-RAY DIFFRACTION	42	( CHAIN F AND RESID 428:457 )	F	428 - 457
43	X-RAY DIFFRACTION	43	( CHAIN F AND RESID 458:461 )	F	458 - 461
44	X-RAY DIFFRACTION	44	( CHAIN F AND RESID 462:504 )	F	462 - 504
45	X-RAY DIFFRACTION	45	( CHAIN F AND RESID 505:509 )	F	505 - 509
46	X-RAY DIFFRACTION	46	( CHAIN F AND RESID 510:516 )	F	510 - 516
47	X-RAY DIFFRACTION	47	( CHAIN F AND RESID 517:533 )	F	517 - 533
48	X-RAY DIFFRACTION	48	( CHAIN G AND RESID 376:399 )	G	376 - 399
49	X-RAY DIFFRACTION	49	( CHAIN G AND RESID 400:406 )	G	400 - 406
50	X-RAY DIFFRACTION	50	( CHAIN G AND RESID 407:441 )	G	407 - 441
51	X-RAY DIFFRACTION	51	( CHAIN G AND RESID 442:473 )	G	442 - 473
52	X-RAY DIFFRACTION	52	( CHAIN G AND RESID 474:479 )	G	474 - 479
53	X-RAY DIFFRACTION	53	( CHAIN G AND RESID 480:502 )	G	480 - 502
54	X-RAY DIFFRACTION	54	( CHAIN G AND RESID 503:509 )	G	503 - 509
55	X-RAY DIFFRACTION	55	( CHAIN G AND RESID 510:518 )	G	510 - 518
56	X-RAY DIFFRACTION	56	( CHAIN G AND RESID 519:533 )	G	519 - 533
57	X-RAY DIFFRACTION	57	( CHAIN H AND RESID 378:395 )	H	378 - 395
58	X-RAY DIFFRACTION	58	( CHAIN H AND RESID 396:457 )	H	396 - 457
59	X-RAY DIFFRACTION	59	( CHAIN H AND RESID 458:461 )	H	458 - 461
60	X-RAY DIFFRACTION	60	( CHAIN H AND RESID 462:484 )	H	462 - 484
61	X-RAY DIFFRACTION	61	( CHAIN H AND RESID 485:491 )	H	485 - 491
62	X-RAY DIFFRACTION	62	( CHAIN H AND RESID 492:514 )	H	492 - 514
63	X-RAY DIFFRACTION	63	( CHAIN H AND RESID 515:521 )	H	515 - 521
64	X-RAY DIFFRACTION	64	( CHAIN H AND RESID 522:533 )	H	522 - 533
65	X-RAY DIFFRACTION	65	( CHAIN I AND RESID 376:393 )	I	376 - 393
66	X-RAY DIFFRACTION	66	( CHAIN I AND RESID 394:420 )	I	394 - 420
67	X-RAY DIFFRACTION	67	( CHAIN I AND RESID 421:427 )	I	421 - 427
68	X-RAY DIFFRACTION	68	( CHAIN I AND RESID 428:457 )	I	428 - 457
69	X-RAY DIFFRACTION	69	( CHAIN I AND RESID 458:461 )	I	458 - 461
70	X-RAY DIFFRACTION	70	( CHAIN I AND RESID 462:509 )	I	462 - 509
71	X-RAY DIFFRACTION	71	( CHAIN I AND RESID 510:515 )	I	510 - 515
72	X-RAY DIFFRACTION	72	( CHAIN I AND RESID 516:533 )	I	516 - 533
73	X-RAY DIFFRACTION	73	( CHAIN K AND RESID 377:394 )	K	377 - 394
74	X-RAY DIFFRACTION	74	( CHAIN K AND RESID 395:427 )	K	395 - 427
75	X-RAY DIFFRACTION	75	( CHAIN K AND RESID 428:457 )	K	428 - 457
76	X-RAY DIFFRACTION	76	( CHAIN K AND RESID 458:461 )	K	458 - 461
77	X-RAY DIFFRACTION	77	( CHAIN K AND RESID 462:478 )	K	462 - 478
78	X-RAY DIFFRACTION	78	( CHAIN K AND RESID 479:486 )	K	479 - 486
79	X-RAY DIFFRACTION	79	( CHAIN K AND RESID 487:514 )	K	487 - 514
80	X-RAY DIFFRACTION	80	( CHAIN K AND RESID 515:533 )	K	515 - 533
81	X-RAY DIFFRACTION	81	( CHAIN L AND RESID 376:392 )	L	376 - 392
82	X-RAY DIFFRACTION	82	( CHAIN L AND RESID 393:404 )	L	393 - 404
83	X-RAY DIFFRACTION	83	( CHAIN L AND RESID 405:456 )	L	405 - 456
84	X-RAY DIFFRACTION	84	( CHAIN L AND RESID 457:460 )	L	457 - 460
85	X-RAY DIFFRACTION	85	( CHAIN L AND RESID 461:501 )	L	461 - 501
86	X-RAY DIFFRACTION	86	( CHAIN L AND RESID 502:514 )	L	502 - 514
87	X-RAY DIFFRACTION	87	( CHAIN L AND RESID 515:521 )	L	515 - 521
88	X-RAY DIFFRACTION	88	( CHAIN L AND RESID 522:533 )	L	522 - 533
89	X-RAY DIFFRACTION	89	( CHAIN M AND RESID 376:401 )	M	376 - 401
90	X-RAY DIFFRACTION	90	( CHAIN M AND RESID 402:407 )	M	402 - 407
91	X-RAY DIFFRACTION	91	( CHAIN M AND RESID 408:457 )	M	408 - 457
92	X-RAY DIFFRACTION	92	( CHAIN M AND RESID 458:461 )	M	458 - 461
93	X-RAY DIFFRACTION	93	( CHAIN M AND RESID 462:508 )	M	462 - 508
94	X-RAY DIFFRACTION	94	( CHAIN M AND RESID 509:515 )	M	509 - 515
95	X-RAY DIFFRACTION	95	( CHAIN M AND RESID 516:529 )	M	516 - 529
96	X-RAY DIFFRACTION	96	( CHAIN M AND RESID 530:534 )	M	530 - 534