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Yorodumi- PDB-5cvg: Crystal Structure of CK2alpha with a novel closed conformation of... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5cvg | ||||||
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Title | Crystal Structure of CK2alpha with a novel closed conformation of the aD loop | ||||||
Components | Casein kinase II subunit alpha | ||||||
Keywords | TRANSFERASE / CK2alpha / CK2a / fragment based drug discovery / high concentration screening / selective ATP competitive inhibitors / surface entrophy reduction | ||||||
Function / homology | Function and homology information regulation of chromosome separation / positive regulation of aggrephagy / WNT mediated activation of DVL / Condensation of Prometaphase Chromosomes / protein kinase CK2 complex / symbiont-mediated disruption of host cell PML body / Receptor Mediated Mitophagy / Sin3-type complex / Synthesis of PC / RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known ...regulation of chromosome separation / positive regulation of aggrephagy / WNT mediated activation of DVL / Condensation of Prometaphase Chromosomes / protein kinase CK2 complex / symbiont-mediated disruption of host cell PML body / Receptor Mediated Mitophagy / Sin3-type complex / Synthesis of PC / RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known / Maturation of hRSV A proteins / negative regulation of apoptotic signaling pathway / positive regulation of Wnt signaling pathway / negative regulation of double-strand break repair via homologous recombination / chaperone-mediated protein folding / negative regulation of ubiquitin-dependent protein catabolic process / Signal transduction by L1 / peptidyl-threonine phosphorylation / Hsp90 protein binding / negative regulation of cysteine-type endopeptidase activity involved in apoptotic process / PML body / Wnt signaling pathway / Regulation of PTEN stability and activity / positive regulation of protein catabolic process / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / KEAP1-NFE2L2 pathway / double-strand break repair / rhythmic process / kinase activity / positive regulation of cell growth / peptidyl-serine phosphorylation / Regulation of TP53 Activity through Phosphorylation / protein stabilization / negative regulation of translation / non-specific serine/threonine protein kinase / regulation of cell cycle / cell cycle / protein phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / apoptotic process / DNA damage response / positive regulation of cell population proliferation / signal transduction / nucleoplasm / ATP binding / identical protein binding / nucleus / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 1.25 Å | ||||||
Authors | Brear, P. / De Fusco, C. / Georgiou, K.H. / Spring, D. / Hyvonen, M. | ||||||
Funding support | United Kingdom, 1items
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Citation | Journal: Chem Sci / Year: 2016 Title: Specific inhibition of CK2 alpha from an anchor outside the active site. Authors: Brear, P. / De Fusco, C. / Hadje Georgiou, K. / Francis-Newton, N.J. / Stubbs, C.J. / Sore, H.F. / Venkitaraman, A.R. / Abell, C. / Spring, D.R. / Hyvonen, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5cvg.cif.gz | 154.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5cvg.ent.gz | 120.1 KB | Display | PDB format |
PDBx/mmJSON format | 5cvg.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 5cvg_validation.pdf.gz | 439.7 KB | Display | wwPDB validaton report |
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Full document | 5cvg_full_validation.pdf.gz | 440.6 KB | Display | |
Data in XML | 5cvg_validation.xml.gz | 16.4 KB | Display | |
Data in CIF | 5cvg_validation.cif.gz | 24.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cv/5cvg ftp://data.pdbj.org/pub/pdb/validation_reports/cv/5cvg | HTTPS FTP |
-Related structure data
Related structure data | 5clpC 5cs6C 5cshC 5cspC 5csvC 5cu3C 5cu4C 5cu6C 5cvfC 5cvhSC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 41380.715 Da / Num. of mol.: 1 Fragment: residues 2-329 and N-terminal extension GSMDIEFDDDADDDGSGSGSGSG Mutation: R21S Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CSNK2A1, CK2A1 / Plasmid: pHAT4 / Production host: Escherichia coli BL21(DE3) (bacteria) References: UniProt: P68400, non-specific serine/threonine protein kinase | ||
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#2: Chemical | ChemComp-ACT / #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.78 Å3/Da / Density % sol: 31.05 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 92mM Mes pH 6.5, 33% glycerol ethoxylate, 750mM ammonium acetate |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I04 / Wavelength: 0.9795 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Sep 14, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 1.25→35.13 Å / Num. obs: 73445 / % possible obs: 90.8 % / Redundancy: 3.2 % / Biso Wilson estimate: 13.48 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.042 / Rpim(I) all: 0.027 / Rsym value: 0.042 / Net I/σ(I): 14.8 / Num. measured all: 231929 |
Reflection shell | Resolution: 1.25→1.254 Å / Redundancy: 2.4 % / Rmerge(I) obs: 0.446 / Mean I/σ(I) obs: 2.1 / Num. measured all: 22648 / Num. unique all: 9390 / CC1/2: 0.829 / Rpim(I) all: 0.271 / Rsym value: 0.446 / Rejects: 0 / % possible all: 48.3 |
-Phasing
Phasing | Method: molecular replacement |
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5CVH Resolution: 1.25→35.13 Å / Cor.coef. Fo:Fc: 0.9693 / Cor.coef. Fo:Fc free: 0.9607 / SU R Cruickshank DPI: 0.045 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.046 / SU Rfree Blow DPI: 0.046 / SU Rfree Cruickshank DPI: 0.045
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Displacement parameters | Biso max: 100.37 Å2 / Biso mean: 18.75 Å2 / Biso min: 6.57 Å2
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Refine analyze | Luzzati coordinate error obs: 0.123 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.25→35.13 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.25→1.28 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Origin x: 46.7756 Å / Origin y: 5.4154 Å / Origin z: 72.9809 Å
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Refinement TLS group | Selection details: { A|* } |