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Open data
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Basic information
Entry | Database: PDB / ID: 5cl1 | ||||||
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Title | Complex structure of Norrin with human Frizzled 4 | ||||||
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![]() | SIGNALING PROTEIN / Wnt / Norrin / Frizzled | ||||||
Function / homology | ![]() retina blood vessel maintenance / cone retinal bipolar cell differentiation / cerebellum vasculature morphogenesis / Wnt signaling pathway, calcium modulating pathway / Norrin signaling pathway / extracellular matrix-cell signaling / re-entry into mitotic cell cycle / progesterone secretion / retinal blood vessel morphogenesis / retinal rod cell differentiation ...retina blood vessel maintenance / cone retinal bipolar cell differentiation / cerebellum vasculature morphogenesis / Wnt signaling pathway, calcium modulating pathway / Norrin signaling pathway / extracellular matrix-cell signaling / re-entry into mitotic cell cycle / progesterone secretion / retinal blood vessel morphogenesis / retinal rod cell differentiation / locomotion involved in locomotory behavior / retina vasculature morphogenesis in camera-type eye / regulation of vascular endothelial growth factor receptor signaling pathway / ubiquitin-dependent endocytosis / Signaling by RNF43 mutants / WNT5A-dependent internalization of FZD4 / retina layer formation / retinal pigment epithelium development / non-canonical Wnt signaling pathway / positive regulation of neuron projection arborization / establishment of blood-retinal barrier / Wnt receptor activity / glycine metabolic process / microglial cell proliferation / endothelial cell differentiation / dendritic spine development / Wnt-protein binding / establishment of blood-brain barrier / vacuole organization / protein targeting to lysosome / microglia differentiation / frizzled binding / Class B/2 (Secretin family receptors) / negative regulation of cell-substrate adhesion / positive regulation of dendrite morphogenesis / lens development in camera-type eye / exploration behavior / cytokine receptor activity / optic nerve development / action potential / smoothened signaling pathway / retinal ganglion cell axon guidance / cytokine binding / decidualization / carbohydrate transmembrane transporter activity / maltose binding / maltose transport / maltodextrin transmembrane transport / response to axon injury / blood vessel remodeling / canonical Wnt signaling pathway / vasculogenesis / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / cellular response to retinoic acid / tricarboxylic acid cycle / Regulation of FZD by ubiquitination / visual perception / glutathione metabolic process / substrate adhesion-dependent cell spreading / transforming growth factor beta receptor signaling pathway / cellular response to leukemia inhibitory factor / Asymmetric localization of PCP proteins / cytokine activity / G protein-coupled receptor activity / PDZ domain binding / sensory perception of sound / clathrin-coated endocytic vesicle membrane / neuron differentiation / Wnt signaling pathway / positive regulation of DNA-binding transcription factor activity / cell-cell junction / Cargo recognition for clathrin-mediated endocytosis / Clathrin-mediated endocytosis / Ca2+ pathway / nervous system development / signaling receptor activity / mitotic cell cycle / amyloid-beta binding / cellular response to hypoxia / outer membrane-bounded periplasmic space / angiogenesis / neuron apoptotic process / collagen-containing extracellular matrix / cell population proliferation / transcription by RNA polymerase II / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / response to hypoxia / protein ubiquitination / positive regulation of cell migration / inflammatory response / protein heterodimerization activity / glutamatergic synapse / ubiquitin protein ligase binding / dendrite / protein-containing complex binding / positive regulation of DNA-templated transcription / cell surface / protein homodimerization activity / extracellular space / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() ![]() | ||||||
![]() | Wang, Z. / Ke, J. / Shen, G. / Cheng, Z. / Xu, H.E. / Xu, W. | ||||||
![]() | ![]() Title: Structural basis of the Norrin-Frizzled 4 interaction. Authors: Shen, G. / Ke, J. / Wang, Z. / Cheng, Z. / Gu, X. / Wei, Y. / Melcher, K. / Xu, H.E. / Xu, W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 462 KB | Display | ![]() |
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PDB format | ![]() | 385.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 470.5 KB | Display | ![]() |
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Full document | ![]() | 473.8 KB | Display | |
Data in XML | ![]() | 39.7 KB | Display | |
Data in CIF | ![]() | 53.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5cm4C ![]() 4my2S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: _ / Refine code: _
NCS ensembles :
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Components
#1: Protein | Mass: 53664.043 Da / Num. of mol.: 2 / Fragment: UNP Q00604 residues 31-133 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Gene: malE, Z5632, ECs5017, NDP, EVR2 / Production host: ![]() #2: Protein | Mass: 15098.341 Da / Num. of mol.: 2 / Fragment: UNP residues 38-160 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #3: Sugar | ChemComp-NAG / |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.62 Å3/Da / Density % sol: 66.03 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 200 mM ammonium sulfate, 100 mM sodium cacodylate trihydrate (pH 6.5), 15% w/v polyethylene glycol 8,000 |
-Data collection
Diffraction | Mean temperature: 100 K | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: May 29, 2015 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Monochromator: Single crystal, cylindrically bent, Si(220) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 3.8→50 Å / Num. obs: 18303 / % possible obs: 90.5 % / Redundancy: 5.9 % / Rmerge(I) obs: 0.264 / Rpim(I) all: 0.117 / Rrim(I) all: 0.28 / Χ2: 1.123 / Net I/av σ(I): 4.476 / Net I/σ(I): 3.5 / Num. measured all: 107966 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1 / Rejects: _
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-Phasing
Phasing | Method: ![]() |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4MY2 Resolution: 3.8→50 Å / Cor.coef. Fo:Fc: 0.879 / Cor.coef. Fo:Fc free: 0.808 / SU B: 144.371 / SU ML: 0.843 / Cross valid method: THROUGHOUT / ESU R Free: 0.905 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : WITH TLS ADDED
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 195.82 Å2 / Biso mean: 112.326 Å2 / Biso min: 71.47 Å2
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Refinement step | Cycle: final / Resolution: 3.8→50 Å
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Refine LS restraints |
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Refine LS restraints NCS | Refine-ID: X-RAY DIFFRACTION / Type: interatomic distance / Weight position: 0.05
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LS refinement shell | Resolution: 3.8→3.892 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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