|Entry||Database: PDB / ID: 5cjp|
|Title||The Structural Basis for Cdc42-Induced Dimerization of IQGAPs|
|Keywords||PROTEIN BINDING / IQGAP2 GAP related domain X-ray GTPase interactions Complex Cdc42|
|Function / homology|
Function and homology information
: / establishment or maintenance of apical/basal cell polarity / thrombin-activated receptor signaling pathway / Arp2/3 complex-mediated actin nucleation / Arp2/3 complex binding / submandibular salivary gland formation / GBD domain binding / regulation of modification of postsynaptic structure / neuron fate determination / modification of synaptic structure ...: / establishment or maintenance of apical/basal cell polarity / thrombin-activated receptor signaling pathway / Arp2/3 complex-mediated actin nucleation / Arp2/3 complex binding / submandibular salivary gland formation / GBD domain binding / regulation of modification of postsynaptic structure / neuron fate determination / modification of synaptic structure / Golgi transport complex / positive regulation of pinocytosis / endothelin receptor signaling pathway involved in heart process / dendritic cell migration / cardiac neural crest cell migration involved in outflow tract morphogenesis / Cdc42 protein signal transduction / organelle transport along microtubule / positive regulation of synapse structural plasticity / GTPase inhibitor activity / storage vacuole / positive regulation of epithelial cell proliferation involved in lung morphogenesis / apolipoprotein A-I receptor binding / positive regulation of pseudopodium assembly / regulation of attachment of spindle microtubules to kinetochore / cardiac conduction system development / Inactivation of CDC42 and RAC1 / cell junction assembly / GTP-dependent protein binding / actin filament branching / establishment of epithelial cell apical/basal polarity / leading edge membrane / regulation of filopodium assembly / positive regulation of intracellular protein transport / establishment of Golgi localization / neuropilin signaling pathway / filopodium assembly / RHO GTPases activate KTN1 / embryonic heart tube development / thioesterase binding / regulation of stress fiber assembly / mitogen-activated protein kinase kinase kinase binding / cell projection assembly / Wnt signaling pathway, planar cell polarity pathway / nuclear migration / regulation of lamellipodium assembly / dendritic spine morphogenesis / regulation of actin cytoskeleton reorganization / adherens junction organization / DCC mediated attractive signaling / positive regulation of filopodium assembly / sprouting angiogenesis / CD28 dependent Vav1 pathway / heart contraction / RHOV GTPase cycle / regulation of mitotic nuclear division / positive regulation of cytokinesis / RHOJ GTPase cycle / regulation of protein binding / positive regulation of phosphatidylinositol 3-kinase activity / positive regulation of lamellipodium assembly / Golgi organization / RHOQ GTPase cycle / establishment or maintenance of cell polarity / RHO GTPases activate PAKs / Myogenesis / microvillus / phosphatidylinositol-3,4,5-trisphosphate binding / positive regulation of actin cytoskeleton reorganization / CDC42 GTPase cycle / RHOG GTPase cycle / macrophage differentiation / RHOU GTPase cycle / RHO GTPases activate IQGAPs / negative regulation of protein-containing complex assembly / RAC2 GTPase cycle / RAC3 GTPase cycle / positive regulation of substrate adhesion-dependent cell spreading / positive regulation of DNA replication / G beta:gamma signalling through CDC42 / RHO GTPases Activate WASPs and WAVEs / regulation of actin cytoskeleton organization / spindle midzone / positive regulation of stress fiber assembly / regulation of GTPase activity / substantia nigra development / phagocytic vesicle / GPVI-mediated activation cascade / EPHB-mediated forward signaling / G protein activity / Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation / RAC1 GTPase cycle / small monomeric GTPase / GTPase activator activity / actin filament organization / secretory granule membrane / filopodium / phagocytosis, engulfment / RHO GTPases Activate Formins / cellular response to interferon-gamma / cell projection
Similarity search - Function
RasGAP protein, C-terminal / RasGAP C-terminus / GTPase Activation - p120GAP; domain 1 / GTPase Activation - p120gap; domain 1 / Cdc42 / Ras GTPase-activating protein, conserved site / Ras GTPase-activating proteins domain signature. / GTPase-activator protein for Ras-like GTPase / Ras GTPase-activating proteins profile. / GTPase-activator protein for Ras-like GTPases ...RasGAP protein, C-terminal / RasGAP C-terminus / GTPase Activation - p120GAP; domain 1 / GTPase Activation - p120gap; domain 1 / Cdc42 / Ras GTPase-activating protein, conserved site / Ras GTPase-activating proteins domain signature. / GTPase-activator protein for Ras-like GTPase / Ras GTPase-activating proteins profile. / GTPase-activator protein for Ras-like GTPases / Ras GTPase-activating domain / small GTPase Rho family profile. / IQ calmodulin-binding motif / Small GTPase Rho / Rho GTPase activation protein / Calponin homology domain / Short calmodulin-binding motif containing conserved Ile and Gln residues. / Calponin homology (CH) domain / Calponin homology domain / Calponin homology (CH) domain profile. / CH domain superfamily / IQ motif profile. / WW/rsp5/WWP domain signature. / IQ motif, EF-hand binding site / WW/rsp5/WWP domain profile. / WW domain / Rho (Ras homology) subfamily of Ras-like small GTPases / Small GTPase / Ras family / Small GTP-binding protein domain / P-loop containing nucleotide triphosphate hydrolases / P-loop containing nucleoside triphosphate hydrolase / Rossmann fold / Orthogonal Bundle / 3-Layer(aba) Sandwich / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
Cell division control protein 42 homolog / GUANOSINE-5'-TRIPHOSPHATE / Cell division cycle 42 (GTP binding protein, 25kDa), isoform CRA_a / Ras GTPase-activating-like protein IQGAP2
Similarity search - Component
|Biological species||Homo sapiens (human)|
|Method||X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.6 Å|
|Authors||Worthylake, D.K. / Boyapati, V.K. / LeCour Jr, L.|
|Funding support|| United States, 1items |
|Citation||Journal: Structure / Year: 2016|
Title: The Structural Basis for Cdc42-Induced Dimerization of IQGAPs.
Authors: LeCour, L. / Boyapati, V.K. / Liu, J. / Li, Z. / Sacks, D.B. / Worthylake, D.K.
|Structure viewer||Molecule: |
Downloads & links
A: Cell division cycle 42 (GTP binding protein, 25kDa), isoform CRA_a
B: Cell division cycle 42 (GTP binding protein, 25kDa), isoform CRA_a
C: Cell division cycle 42 (GTP binding protein, 25kDa), isoform CRA_a
D: Cell division cycle 42 (GTP binding protein, 25kDa), isoform CRA_a
E: Ras GTPase-activating-like protein IQGAP2
F: Ras GTPase-activating-like protein IQGAP2
Mass: 19758.748 Da / Num. of mol.: 4 / Mutation: Q61L
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: CDC42, hCG_1981007 / Plasmid: pPRO EX HTb
Details (production host): plasmid encodes a TEV protease cleavable 6xHis tag which leaves a Gly-Ala cloning artifact preceding the initiator methionine
Cell line (production host): RIL / Production host: Escherichia coli K-12 (bacteria) / Strain (production host): BL21 / Variant (production host): DE3 / References: UniProt: A0A024RAE4, UniProt: P60953*PLUS
Mass: 44058.094 Da / Num. of mol.: 2 / Fragment: unp reesidues 875-1258
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: IQGAP2 / Plasmid: pET Trx
Details (production host): kanamycin, encodes E. coli thioredoxin and a TEV protease-cleavable 6xHis tag N-terminal to the IQGAP2 GAP-related domain
Cell line (production host): RIL / Production host: Escherichia coli K-12 (bacteria) / Strain (production host): BL21 / Variant (production host): DE3 / References: UniProt: Q13576
Mass: 523.180 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C10H16N5O14P3 / Comment: GTP, energy-carrying molecule*YM
|#5: Water|| ChemComp-HOH / |
|Experiment||Method: X-RAY DIFFRACTION / Number of used crystals: 1|
|Crystal||Density Matthews: 2.93 Å3/Da / Density % sol: 58.01 %|
|Crystal grow||Temperature: 277.15 K / Method: vapor diffusion, sitting drop / pH: 6.5 |
Details: 28.5% (v/v) polyethylene glycol (PEG) MW 400, 100mM 2-(N-morpholino)ethanesulfonic acid (MES) pH 6.5 and 40mM MgCl2.
PH range: 6.5-6.5
|Diffraction||Mean temperature: 100 K|
|Diffraction source||Source: SYNCHROTRON / Site: APS / Beamline: 31-ID / Wavelength: 0.97931 Å|
|Detector||Type: RAYONIX MX225HE / Detector: CCD / Date: Feb 12, 2014|
|Radiation||Monochromator: Kohzu HLD-4 Double Crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray|
|Radiation wavelength||Wavelength: 0.97931 Å / Relative weight: 1|
|Reflection||Resolution: 2.57→20 Å / Num. obs: 61742 / % possible obs: 99.7 % / Redundancy: 7.3 % / Net I/av σ(I): 2.9 / Net I/σ(I): 10.5|
|Reflection shell||Resolution: 2.57→2.71 Å / Redundancy: 7.2 % / Rmerge(I) obs: 0.81 / Mean I/σ(I) obs: 2.9 / % possible all: 100|
|Refinement||Resolution: 2.6→20 Å / Cross valid method: FREE R-VALUE / σ(F): 0 |
|Solvent computation||Bsol: 33.9884 Å2|
|Displacement parameters||Biso max: 198.96 Å2 / Biso mean: 72.6528 Å2 / Biso min: 19.35 Å2|
|Refinement step||Cycle: final / Resolution: 2.6→20 Å|
|Refine LS restraints||Type: c_angle_d / Dev ideal: 1.314|
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