温度: 289 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 7.5 詳細: 0.2 ul of 10.5 mg/ml protein in 20 mM HEPES pH 7.5, 150 mM NaCl, 10% Glycerol, 0.1% Sodium Azide and 0.5 mM TCEP were mixed with 0.2 ul of the MCSG Suite 2 condition # 72 (20%w/v PEG 3350, 0. ...詳細: 0.2 ul of 10.5 mg/ml protein in 20 mM HEPES pH 7.5, 150 mM NaCl, 10% Glycerol, 0.1% Sodium Azide and 0.5 mM TCEP were mixed with 0.2 ul of the MCSG Suite 2 condition # 72 (20%w/v PEG 3350, 0.2M Li nitrate) and equilibrated against 1.5 M NaCl solution in 96 Well 3 drop Crystallization Plate (Swissci). Before crystallization protein was incubated with 1/50 v/v of 2 mg/ml chymotrypsin
解像度: 2.2→50 Å / Cor.coef. Fo:Fc: 0.964 / Cor.coef. Fo:Fc free: 0.938 / WRfactor Rfree: 0.2498 / WRfactor Rwork: 0.1866 / FOM work R set: 0.7138 / SU B: 24.162 / SU ML: 0.266 / SU R Cruickshank DPI: 0.3836 / SU Rfree: 0.2475 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 0.384 / ESU R Free: 0.248 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: U VALUES : WITH TLS ADDED HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.252
1253
4.8 %
RANDOM
Rwork
0.1945
-
-
-
obs
0.1973
26059
96.36 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK