分子量: 101855.594 Da / 分子数: 1 / 変異: H233Y, E230Q,H233Y, E230Q,H233Y, E230Q,H233Y, E230Q / 由来タイプ: 組換発現 詳細: Engineered fragment of the botulinum neurotoxin type D with inactive catalytic domain and translocation domain. An engineered linker region is present between the two domains. Residues 442- ...詳細: Engineered fragment of the botulinum neurotoxin type D with inactive catalytic domain and translocation domain. An engineered linker region is present between the two domains. Residues 442-457, 494-510, 872-884 were disordered. 由来: (組換発現) Clostridium botulinum (ボツリヌス菌) 遺伝子: botD / 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / 参照: UniProt: P19321, bontoxilysin
解像度: 2.3→150 Å / Cor.coef. Fo:Fc: 0.959 / Cor.coef. Fo:Fc free: 0.951 / SU B: 22.239 / SU ML: 0.205 / 交差検証法: THROUGHOUT / ESU R: 0.185 / ESU R Free: 0.169 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.2467
4277
5 %
RANDOM
Rwork
0.22015
-
-
-
obs
0.22144
82020
98.4 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK