+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 5b7a | |||||||||
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タイトル | SOLUTION STRUCTURE OF LYS37 ACETYLATED HUMAN SUMO1 | |||||||||
要素 | Small ubiquitin-related modifier 1 | |||||||||
キーワード | STRUCTURAL GENOMICS (構造ゲノミクス) / UBIQUITIN-LIKE PROTEIN (ユビキチン様タンパク質) / ACETYLATED PROTEIN (アセチル化) | |||||||||
機能・相同性 | 機能・相同性情報 protein localization to nuclear pore / negative regulation of transcription by transcription factor localization / SUMOylation of nuclear envelope proteins / Negative regulation of activity of TFAP2 (AP-2) family transcription factors / SUMO is proteolytically processed / negative regulation of delayed rectifier potassium channel activity / SUMO is conjugated to E1 (UBA2:SAE1) / nuclear stress granule / PML body organization / SUMO is transferred from E1 to E2 (UBE2I, UBC9) ...protein localization to nuclear pore / negative regulation of transcription by transcription factor localization / SUMOylation of nuclear envelope proteins / Negative regulation of activity of TFAP2 (AP-2) family transcription factors / SUMO is proteolytically processed / negative regulation of delayed rectifier potassium channel activity / SUMO is conjugated to E1 (UBA2:SAE1) / nuclear stress granule / PML body organization / SUMO is transferred from E1 to E2 (UBE2I, UBC9) / negative regulation of action potential / small protein activating enzyme binding / セプチン / regulation of calcium ion transmembrane transport / SUMOylation of DNA methylation proteins / SUMOylation of immune response proteins / XY body / SUMOylation of SUMOylation proteins / Maturation of nucleoprotein / SUMOylation of RNA binding proteins / regulation of cardiac muscle cell contraction / Postmitotic nuclear pore complex (NPC) reformation / Maturation of nucleoprotein / negative regulation of protein import into nucleus / ubiquitin-specific protease binding / roof of mouth development / SUMOylation of ubiquitinylation proteins / ubiquitin-like protein ligase binding / negative regulation of DNA binding / SUMOylation of DNA replication proteins / protein sumoylation / transcription factor binding / SUMOylation of transcription factors / potassium channel regulator activity / SUMOylation of DNA damage response and repair proteins / Regulation of IFNG signaling / 核膜孔 / cellular response to cadmium ion / SUMOylation of chromatin organization proteins / SUMOylation of transcription cofactors / positive regulation of protein-containing complex assembly / SUMOylation of intracellular receptors / PKR-mediated signaling / negative regulation of DNA-binding transcription factor activity / PML body / Formation of Incision Complex in GG-NER / protein tag activity / regulation of protein localization / positive regulation of proteasomal ubiquitin-dependent protein catabolic process / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / cellular response to heat / 核膜 / nuclear body / protein stabilization / nuclear speck / DNA修復 / negative regulation of DNA-templated transcription / ubiquitin protein ligase binding / 核小体 / enzyme binding / RNA binding / 核質 / 細胞核 / 細胞膜 / 細胞質基質 類似検索 - 分子機能 | |||||||||
生物種 | Homo sapiens (ヒト) | |||||||||
手法 | 溶液NMR / TORSION ANGLE DYNAMICS, DGSA- DISTANCE GEOMETRY SIMULATED ANNEALING | |||||||||
データ登録者 | Naik, M.T. / Naik, N. / Shih, H. / Huang, T. | |||||||||
引用 | ジャーナル: To Be Published タイトル: Structures Of Human Sumo 著者: Naik, M.T. / Naik, N. / Shih, H. / Huang, T. | |||||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 5b7a.cif.gz | 607.6 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb5b7a.ent.gz | 507.6 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 5b7a.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/b7/5b7a ftp://data.pdbj.org/pub/pdb/validation_reports/b7/5b7a | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質 | 分子量: 12809.266 Da / 分子数: 1 / 断片: UNP residues 1-97 / 由来タイプ: 組換発現 詳細: Lys37 acetylated mature SMALL UBIQUITIN-RELATED MODIFIER 1 ( SUMO1) Residues 1-14 (MGSSHHHHHHSQDP) represent a non-native purification tag. These residues were neither assigned nor included ...詳細: Lys37 acetylated mature SMALL UBIQUITIN-RELATED MODIFIER 1 ( SUMO1) Residues 1-14 (MGSSHHHHHHSQDP) represent a non-native purification tag. These residues were neither assigned nor included in structure calculation. 由来: (組換発現) Homo sapiens (ヒト) 解説: PLASMID PCDF PYLT-1 WITH SUMO INSERT WITH K37STOP MUTATION (WITH AMBER CODON) AND PACKRS-3 AS DESCRIBED IN NEUMANN ET AL., MOL CELL, 36, 153, 2009 遺伝子: SUMO1, SMT3C, SMT3H3, UBL1, OK/SW-cl.43 詳細 (発現宿主): Plasmid pCDF PylT-1 with SUMO insert with K37STOP mutation (with amber codon) and pAcKRS-3 as described in Neumann et al., Mol Cell, 36, 153, 2009 発現宿主: ESCHERICHIA COLI BL21(DE3) (大腸菌) / 株 (発現宿主): BL21(DE3) / 参照: UniProt: P63165 |
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-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR実験 |
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NMR実験の詳細 | Text: NMR DATA WAS ACQUIRED AT 295K USING SHIGEMI NMR TUBES. |
-試料調製
詳細 |
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試料 |
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