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- PDB-5b00: Structure of the prenyltransferase MoeN5 in complex with geranyl ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5b00 | ||||||
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Title | Structure of the prenyltransferase MoeN5 in complex with geranyl pyrophosphate | ||||||
![]() | MoeN5 | ||||||
![]() | TRANSFERASE / prenyltransferase / alpha-helical fold | ||||||
Function / homology | Farnesyl Diphosphate Synthase / Farnesyl Diphosphate Synthase / Isoprenoid synthase domain superfamily / Orthogonal Bundle / Mainly Alpha / GERANYL DIPHOSPHATE / MoeN5![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ko, T.-P. / Zhang, L. / Chen, C.-C. / Guo, R.-T. | ||||||
![]() | ![]() Title: Moenomycin Biosynthesis: Structure and Mechanism of Action of the Prenyltransferase MoeN5. Authors: Zhang, L. / Chen, C.C. / Ko, T.P. / Huang, J.W. / Zheng, Y. / Liu, W. / Wang, I. / Malwal, S.R. / Feng, X. / Wang, K. / Huang, C.H. / Hsu, S.T. / Wang, A.H. / Oldfield, E. / Guo, R.T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 164.9 KB | Display | ![]() |
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PDB format | ![]() | 130.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5b02C ![]() 5b03C ![]() 5b0iC ![]() 5b0jC ![]() 5b0kC ![]() 5b0lC ![]() 5b0mC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 32258.320 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.9 Å3/Da / Density % sol: 57.57 % / Description: hicup uppsala |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 9.1 / Details: 0.2 M Na2HPO4, 16% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jan 14, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.95→25 Å / Num. obs: 23648 / % possible obs: 99 % / Redundancy: 4.5 % / Rmerge(I) obs: 0.087 / Net I/σ(I): 19.9 |
Reflection shell | Resolution: 2.95→3.06 Å / Redundancy: 4.3 % / Rmerge(I) obs: 0.453 / Mean I/σ(I) obs: 3.5 / % possible all: 93.4 |
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Processing
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Refinement | Method to determine structure: ![]()
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Refine analyze | Luzzati coordinate error obs: 0.67 Å / Luzzati d res low obs: 5 Å / Luzzati sigma a obs: 1.16 Å | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.95→25 Å
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