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- PDB-5ann: Structure of fructofuranosidase from Xanthophyllomyces dendrorhous -
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Open data
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Basic information
Entry | Database: PDB / ID: 5ann | |||||||||
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Title | Structure of fructofuranosidase from Xanthophyllomyces dendrorhous | |||||||||
![]() | BETA-FRUCTOFURANOSIDASE | |||||||||
![]() | HYDROLASE / DIMERIZATION / QUATERNARY / XANTHOPHYLLOMYCES DENDR GLYCOSIDE HYDROLASES / FUNGAL PROTEINS / KINETICS / PROTEIN CONFORMATION / SUBSTRATE SPECIFICITY / INVERTASE / PREBIOTICS | |||||||||
Function / homology | ![]() sucrose alpha-glucosidase activity / sucrose catabolic process / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Ramirez-Escudero, M. / Sanz-Aparicio, J. | |||||||||
![]() | ![]() Title: Structural Analysis of Beta-Fructofuranosidase from Xanthophyllomyces Dendrorhous Reveals Unique Features and the Crucial Role of N-Glycosylation in Oligomerization and Activity Authors: Ramirez-Escudero, M. / Gimeno-Perez, M. / Gonzalez, B. / Linde, D. / Merdzo, Z. / Fernandez-Lobato, M. / Sanz-Aparicio, J. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 292 KB | Display | ![]() |
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PDB format | ![]() | 240.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.5 MB | Display | ![]() |
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Full document | ![]() | 2.5 MB | Display | |
Data in XML | ![]() | 60 KB | Display | |
Data in CIF | ![]() | 90 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5fixC ![]() 5fk7C ![]() 5fk8C ![]() 5fkbC ![]() 5fkcC ![]() 5fmbC ![]() 5fmcC ![]() 5fmdC ![]() 3ldkS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
NCS oper: (Code: given / Matrix: (-1), |
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Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 72065.188 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() |
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-Sugars , 4 types, 32 molecules ![](data/chem/img/NAG.gif)
#2: Polysaccharide | Source method: isolated from a genetically manipulated source #3: Polysaccharide | Source method: isolated from a genetically manipulated source #4: Polysaccharide | Source method: isolated from a genetically manipulated source #6: Sugar | ChemComp-NAG / |
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-Non-polymers , 2 types, 1221 molecules ![](data/chem/img/GOL.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/HOH.gif)
#5: Chemical | ChemComp-GOL / #7: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.07 Å3/Da / Density % sol: 40.67 % / Description: NONE |
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Crystal grow | pH: 7.5 Details: PROTEIN WAS CRYSTALLIZED FROM 1.3M SODIUM CITRATE TRIBASIC DIHYDRATE, 0.1M HEPES PH 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jun 14, 2010 / Details: MIRRORS |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0395 Å / Relative weight: 1 |
Reflection | Resolution: 2.14→61.92 Å / Num. obs: 117631 / % possible obs: 99.9 % / Observed criterion σ(I): 0 / Redundancy: 7.1 % / Rmerge(I) obs: 0.11 / Net I/σ(I): 12.8 |
Reflection shell | Resolution: 2.14→2.26 Å / Redundancy: 6.8 % / Rmerge(I) obs: 0.39 / Mean I/σ(I) obs: 4.3 / % possible all: 99.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 3LDK Resolution: 2.14→145.77 Å / Cor.coef. Fo:Fc: 0.953 / Cor.coef. Fo:Fc free: 0.943 / SU B: 3.259 / SU ML: 0.085 / Cross valid method: THROUGHOUT / ESU R: 0.15 / ESU R Free: 0.129 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES REFINED INDIVIDUALLY, RESIDUES 1-41 ARE DISORDERED
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 21.193 Å2
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Refinement step | Cycle: LAST / Resolution: 2.14→145.77 Å
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Refine LS restraints |
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