+Open data
-Basic information
Entry | Database: PDB / ID: 5aj1 | ||||||
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Title | Solution Structure of the Smarc Domain | ||||||
Components | SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY B MEMBER 1 | ||||||
Keywords | STRUCTURAL PROTEIN | ||||||
Function / homology | Function and homology information single stranded viral RNA replication via double stranded DNA intermediate / positive regulation of pseudohyphal growth by positive regulation of transcription from RNA polymerase II promoter / positive regulation of glucose mediated signaling pathway / bBAF complex / blastocyst hatching / npBAF complex / brahma complex / nBAF complex / positive regulation of transcription of nucleolar large rRNA by RNA polymerase I / regulation of G0 to G1 transition ...single stranded viral RNA replication via double stranded DNA intermediate / positive regulation of pseudohyphal growth by positive regulation of transcription from RNA polymerase II promoter / positive regulation of glucose mediated signaling pathway / bBAF complex / blastocyst hatching / npBAF complex / brahma complex / nBAF complex / positive regulation of transcription of nucleolar large rRNA by RNA polymerase I / regulation of G0 to G1 transition / Tat protein binding / XY body / nucleosome disassembly / regulation of nucleotide-excision repair / RSC-type complex / hepatocyte differentiation / RNA polymerase I preinitiation complex assembly / positive regulation by host of viral transcription / SWI/SNF complex / regulation of mitotic metaphase/anaphase transition / positive regulation of double-strand break repair / germ cell nucleus / positive regulation of T cell differentiation / nuclear chromosome / positive regulation of stem cell population maintenance / RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known / regulation of G1/S transition of mitotic cell cycle / positive regulation of myoblast differentiation / transcription initiation-coupled chromatin remodeling / positive regulation of cell differentiation / kinetochore / fibrillar center / RMTs methylate histone arginines / nuclear matrix / DNA integration / positive regulation of DNA-binding transcription factor activity / p53 binding / nervous system development / transcription coactivator activity / chromatin remodeling / cell cycle / negative regulation of cell population proliferation / intracellular membrane-bounded organelle / chromatin / nucleolus / regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / protein-containing complex / DNA binding / nucleoplasm / identical protein binding / nucleus Similarity search - Function | ||||||
Biological species | HOMO SAPIENS (human) | ||||||
Method | SOLUTION NMR / CNS | ||||||
Authors | Allen, M.D. / Freund, S.M.V. / Zinzalla, G. / Bycroft, M. | ||||||
Citation | Journal: Structure / Year: 2015 Title: The Swi/Snf Subunit Ini1 Contains an N-Terminal Winged Helix DNA Binding Domain that is a Target for Mutations in Schwannomatosis. Authors: Allen, M.D. / Freund, S.M.V. / Zinzalla, G. / Bycroft, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5aj1.cif.gz | 705.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5aj1.ent.gz | 594.3 KB | Display | PDB format |
PDBx/mmJSON format | 5aj1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/aj/5aj1 ftp://data.pdbj.org/pub/pdb/validation_reports/aj/5aj1 | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 12995.907 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: GGS AT THE N-TERMINUS RESULTS FROM A TEV CLEAVAGE SITE. Source: (gene. exp.) HOMO SAPIENS (human) / Production host: ESCHERICHIA COLI BL21(DE3) (bacteria) / Variant (production host): C41 / References: UniProt: Q12824 |
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Sequence details | GGS AT THE N-TERMINUS RESULTS FROM A TEV PROTEASE CLEAVAGE SITE |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: THE DOMAIN WAS ASSIGNED USING 13C, 15N-LABELED PROTEIN AND THE STRUCTURE WAS DETERMINED USING UNLABELLED PROTEIN. |
-Sample preparation
Details | Contents: 95% WATER/5% D2O |
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Sample conditions | Ionic strength: 100 mM / pH: 6.5 / Pressure: 1.0 atm / Temperature: 293.0 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: CNS / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE | ||||||||||||
NMR ensemble | Conformer selection criteria: NO VIOLATIONS / Conformers calculated total number: 20 / Conformers submitted total number: 20 |