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- PDB-5aby: Complex of C. elegans eIF4E-3 with the 4E-binding protein Mextli -

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Basic information

Entry
Database: PDB / ID: 5aby
TitleComplex of C. elegans eIF4E-3 with the 4E-binding protein Mextli
Components
  • 4E-BINDING PROTEIN MEXTLI
  • EUKARYOTIC TRANSLATION INITIATION FACTOR 4E-3
KeywordsTRANSLATION / GENE REGULATION / CAP BINDING PROTEIN / 4E BINDING PROTEIN
Function / homology
Function and homology information


translation initiation complex / L13a-mediated translational silencing of Ceruloplasmin expression / : / : / : / Translation initiation complex formation / Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S / Ribosomal scanning and start codon recognition / regulation of formation of translation initiation ternary complex / ISG15 antiviral mechanism ...translation initiation complex / L13a-mediated translational silencing of Ceruloplasmin expression / : / : / : / Translation initiation complex formation / Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S / Ribosomal scanning and start codon recognition / regulation of formation of translation initiation ternary complex / ISG15 antiviral mechanism / 21U-RNA metabolic process / RNA cap binding complex / eukaryotic initiation factor 4E binding / eukaryotic translation initiation factor 4F complex / regulatory ncRNA-mediated gene silencing / RNA 7-methylguanosine cap binding / embryo development ending in birth or egg hatching / translational initiation / translation initiation factor activity / positive regulation of translation / perinuclear region of cytoplasm / RNA binding / cytoplasm
Similarity search - Function
Eukaryotic translation initiation factor 4E-binding protein Mextli / RNA Cap, Translation Initiation Factor Eif4e / RNA Cap, Translation Initiation Factor Eif4e / Eukaryotic translation initiation factor 4E (eIF-4E), conserved site / Eukaryotic initiation factor 4E signature. / Translation Initiation factor eIF- 4e / Eukaryotic initiation factor 4E / Translation Initiation factor eIF- 4e-like / KH domain / K Homology domain, type 1 ...Eukaryotic translation initiation factor 4E-binding protein Mextli / RNA Cap, Translation Initiation Factor Eif4e / RNA Cap, Translation Initiation Factor Eif4e / Eukaryotic translation initiation factor 4E (eIF-4E), conserved site / Eukaryotic initiation factor 4E signature. / Translation Initiation factor eIF- 4e / Eukaryotic initiation factor 4E / Translation Initiation factor eIF- 4e-like / KH domain / K Homology domain, type 1 / Type-1 KH domain profile. / K Homology domain, type 1 superfamily / K Homology domain / K homology RNA-binding domain / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
Eukaryotic translation initiation factor 4E-3 / Eukaryotic translation initiation factor 4E-binding protein Mextli homolog
Similarity search - Component
Biological speciesCAENORHABDITIS ELEGANS (invertebrata)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.95 Å
AuthorsPeter, D. / Weichenrieder, O.
CitationJournal: Genes Dev. / Year: 2015
Title: Mextli Proteins Use Both Canonical Bipartite and Novel Tripartite Binding Modes to Form Eif4E Complexes that Display Differential Sensitivity to 4E-BP Regulation
Authors: Peter, D. / Weber, R. / Koene, C. / Chung, M.-Y. / Ebertsch, L. / Truffault, V. / Weichenrieder, O. / Igreja, C. / Izaurralde, E.
History
DepositionAug 9, 2015Deposition site: PDBE / Processing site: PDBE
Revision 1.0Sep 2, 2015Provider: repository / Type: Initial release
Revision 1.1Sep 23, 2015Group: Database references
Revision 2.0Oct 23, 2019Group: Atomic model / Data collection / Other / Category: atom_site / pdbx_database_status
Item: _atom_site.B_iso_or_equiv / _atom_site.Cartn_x ..._atom_site.B_iso_or_equiv / _atom_site.Cartn_x / _atom_site.Cartn_y / _atom_site.Cartn_z / _pdbx_database_status.status_code_sf
Revision 2.1Jan 10, 2024Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_conn / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id
Remark 650 HELIX DETERMINATION METHOD: AUTHOR PROVIDED.

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: EUKARYOTIC TRANSLATION INITIATION FACTOR 4E-3
B: 4E-BINDING PROTEIN MEXTLI
C: EUKARYOTIC TRANSLATION INITIATION FACTOR 4E-3
D: 4E-BINDING PROTEIN MEXTLI
E: EUKARYOTIC TRANSLATION INITIATION FACTOR 4E-3
F: 4E-BINDING PROTEIN MEXTLI
hetero molecules


Theoretical massNumber of molelcules
Total (without water)81,67911
Polymers81,3546
Non-polymers3255
Water7,819434
1
A: EUKARYOTIC TRANSLATION INITIATION FACTOR 4E-3
B: 4E-BINDING PROTEIN MEXTLI
hetero molecules


Theoretical massNumber of molelcules
Total (without water)27,2103
Polymers27,1182
Non-polymers921
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2870 Å2
ΔGint-17.9 kcal/mol
Surface area11330 Å2
MethodPISA
2
C: EUKARYOTIC TRANSLATION INITIATION FACTOR 4E-3
D: 4E-BINDING PROTEIN MEXTLI
hetero molecules


Theoretical massNumber of molelcules
Total (without water)27,2595
Polymers27,1182
Non-polymers1413
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3050 Å2
ΔGint-29.1 kcal/mol
Surface area11460 Å2
MethodPISA
3
E: EUKARYOTIC TRANSLATION INITIATION FACTOR 4E-3
F: 4E-BINDING PROTEIN MEXTLI
hetero molecules


Theoretical massNumber of molelcules
Total (without water)27,2103
Polymers27,1182
Non-polymers921
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2930 Å2
ΔGint-18.3 kcal/mol
Surface area11400 Å2
MethodPISA
Unit cell
Length a, b, c (Å)91.240, 157.580, 55.180
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number18
Space group name H-MP21212
Components on special symmetry positions
IDModelComponents
11C-2051-

HOH

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Components

#1: Protein EUKARYOTIC TRANSLATION INITIATION FACTOR 4E-3 / EIF-4E-3 / EIF4E-3 / EIF-4F 25 KDA SUBUNIT / MRNA CAP-BINDING PROTEIN


Mass: 22377.488 Da / Num. of mol.: 3 / Fragment: UNP RESIDUES 30-215
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) CAENORHABDITIS ELEGANS (invertebrata) / Plasmid: PETMCN (PNYC) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): STAR / References: UniProt: O61955
#2: Protein/peptide 4E-BINDING PROTEIN MEXTLI


Mass: 4740.511 Da / Num. of mol.: 3 / Fragment: UNP RESIDUES 471-507
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) CAENORHABDITIS ELEGANS (invertebrata) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): STAR / References: UniProt: Q9XW13
#3: Chemical ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C3H8O3
#4: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Mg
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 434 / Source method: isolated from a natural source / Formula: H2O
Sequence detailsTHE FIRST FOUR RESIDUES OF CHAINS A, C, E REMAIN FROM THE EXPRESSION TAG. THE FIRST FOUR RESIDUES ...THE FIRST FOUR RESIDUES OF CHAINS A, C, E REMAIN FROM THE EXPRESSION TAG. THE FIRST FOUR RESIDUES OF CHAINS B, D, F REMAIN FROM THE EXPRESSION TAG.

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.67 Å3/Da / Density % sol: 54 % / Description: NONE
Crystal growpH: 8 / Details: 0.1M TRIS, PH=8.0, 0.2M MGCL2, 17% PEG6000

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X10SA / Wavelength: 1.00001
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: May 27, 2015 / Details: DYNAMICALLY BENDABLE MIRRORS
RadiationMonochromator: SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.00001 Å / Relative weight: 1
ReflectionResolution: 1.95→47.2 Å / Num. obs: 58918 / % possible obs: 100 % / Observed criterion σ(I): -3 / Redundancy: 8.3 % / Biso Wilson estimate: 29.49 Å2 / Rsym value: 0.12 / Net I/σ(I): 11.8
Reflection shellResolution: 1.95→2 Å / Redundancy: 8.6 % / Mean I/σ(I) obs: 2 / Rsym value: 1.17 / % possible all: 100

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Processing

Software
NameVersionClassification
PHENIX(PHENIX.REFINE: 1.9_1692)refinement
XDSdata reduction
XSCALEdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 5ABX

5abx


Resolution: 1.95→45.522 Å / SU ML: 0.2 / σ(F): 1.36 / Phase error: 20.91 / Stereochemistry target values: ML
Details: HYDROGENS WERE REFINED IN THE RIDING POSITIONS. THE FOLLOWING RESIDUES ARE DISORDERED. CHAIN A, RESIDUES 203 TO 209. CHAIN C, RESIDUES 203 TO 208. CHAIN E, RESIDUES 202 TO 209. CHAIN F, RESIDUE 507.
RfactorNum. reflection% reflection
Rfree0.2071 2937 5 %
Rwork0.1719 --
obs0.1737 58873 99.96 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 34.1 Å2
Refinement stepCycle: LAST / Resolution: 1.95→45.522 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms5464 0 20 434 5918
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0085604
X-RAY DIFFRACTIONf_angle_d1.0457574
X-RAY DIFFRACTIONf_dihedral_angle_d13.3352109
X-RAY DIFFRACTIONf_chiral_restr0.042809
X-RAY DIFFRACTIONf_plane_restr0.004963
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.95-1.9820.27471410.23832635X-RAY DIFFRACTION100
1.982-2.01610.29131270.2272618X-RAY DIFFRACTION100
2.0161-2.05280.28291350.22062643X-RAY DIFFRACTION100
2.0528-2.09230.26251600.21642600X-RAY DIFFRACTION100
2.0923-2.1350.27331400.21012651X-RAY DIFFRACTION100
2.135-2.18140.24651430.19252577X-RAY DIFFRACTION100
2.1814-2.23220.23931390.19112657X-RAY DIFFRACTION100
2.2322-2.2880.22921330.17822645X-RAY DIFFRACTION100
2.288-2.34980.23591510.18222607X-RAY DIFFRACTION100
2.3498-2.4190.25191420.17842650X-RAY DIFFRACTION100
2.419-2.49710.18711500.1742630X-RAY DIFFRACTION100
2.4971-2.58630.21241110.16562657X-RAY DIFFRACTION100
2.5863-2.68980.21451210.17142673X-RAY DIFFRACTION100
2.6898-2.81220.25541220.17872677X-RAY DIFFRACTION100
2.8122-2.96050.23041440.17872663X-RAY DIFFRACTION100
2.9605-3.14590.21751230.18162692X-RAY DIFFRACTION100
3.1459-3.38870.22311490.18032657X-RAY DIFFRACTION100
3.3887-3.72960.18541310.1592716X-RAY DIFFRACTION100
3.7296-4.2690.1791530.1422706X-RAY DIFFRACTION100
4.269-5.37710.16231510.13542725X-RAY DIFFRACTION100
5.3771-45.53440.17981710.18412857X-RAY DIFFRACTION100

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