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- PDB-5a5h: The crystal structure of the GST-like domains complex of EPRS C92... -

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Basic information

Entry
Database: PDB / ID: 5a5h
TitleThe crystal structure of the GST-like domains complex of EPRS C92SC105SC123S mutant-AIMP2
Components
  • AMINOACYL TRNA SYNTHASE COMPLEX-INTERACTING MULTIFUNCTIONA L PROTEIN 2
  • BIFUNCTIONAL GLUTAMATE/PROLINE--TRNA LIGASE
KeywordsTRANSLATION / EPRS / AIMP2 / GST-LIKE DOMAIN
Function / homology
Function and homology information


type II pneumocyte differentiation / regulation of long-chain fatty acid import into cell / glutamate-tRNA ligase / Selenoamino acid metabolism / glutamate-tRNA ligase activity / proline-tRNA ligase / glutamyl-tRNA aminoacylation / proline-tRNA ligase activity / prolyl-tRNA aminoacylation / tRNA modification in the nucleus and cytosol ...type II pneumocyte differentiation / regulation of long-chain fatty acid import into cell / glutamate-tRNA ligase / Selenoamino acid metabolism / glutamate-tRNA ligase activity / proline-tRNA ligase / glutamyl-tRNA aminoacylation / proline-tRNA ligase activity / prolyl-tRNA aminoacylation / tRNA modification in the nucleus and cytosol / Cytosolic tRNA aminoacylation / aminoacyl-tRNA synthetase multienzyme complex / tRNA aminoacylation for protein translation / GAIT complex / Transcriptional and post-translational regulation of MITF-M expression and activity / positive regulation of protein ubiquitination / cellular response to type II interferon / cellular response to insulin stimulus / RNA stem-loop binding / GTPase binding / protein-containing complex assembly / molecular adaptor activity / negative regulation of translation / protein ubiquitination / translation / ribonucleoprotein complex / negative regulation of cell population proliferation / apoptotic process / protein homodimerization activity / zinc ion binding / ATP binding / identical protein binding / nucleus / membrane / plasma membrane / cytosol / cytoplasm
Similarity search - Function
Glutathione S-transferase Yfyf (Class Pi); Chain A, domain 2 - #130 / AIMP2, lysyl-tRNA synthetase binding domain / AIMP2, thioredoxin-like domain / Aminoacyl tRNA synthase complex-interacting multifunctional protein 2 / AIMP2 lysyl-tRNA synthetase binding domain / Thioredoxin-like domain / Glutamyl-tRNA synthetase, archaeal/eukaryotic cytosolic / : / Nuclear-export cofactor Arc1p / WHEP-TRS domain ...Glutathione S-transferase Yfyf (Class Pi); Chain A, domain 2 - #130 / AIMP2, lysyl-tRNA synthetase binding domain / AIMP2, thioredoxin-like domain / Aminoacyl tRNA synthase complex-interacting multifunctional protein 2 / AIMP2 lysyl-tRNA synthetase binding domain / Thioredoxin-like domain / Glutamyl-tRNA synthetase, archaeal/eukaryotic cytosolic / : / Nuclear-export cofactor Arc1p / WHEP-TRS domain / Glutamyl/glutaminyl-tRNA synthetase, class Ib, anti-codon binding domain / : / WHEP-TRS domain / tRNA synthetases class I (E and Q), anti-codon binding domain / tRNA synthetases class I (E and Q), anti-codon binding domain / WHEP-TRS domain signature. / WHEP-TRS domain profile. / WHEP-TRS / Prolyl-tRNA synthetase, catalytic domain / Proline-tRNA ligase, class II, C-terminal / Prolyl-tRNA synthetase, C-terminal / Prolyl-tRNA synthetase, C-terminal / Proline-tRNA ligase, class IIa, archaeal-type / Prolyl-tRNA synthetase, class II / Glutamyl/glutaminyl-tRNA synthetase / Glutamyl/glutaminyl-tRNA synthetase, class Ib, catalytic domain / tRNA synthetases class I (E and Q), catalytic domain / Aminoacyl-tRNA synthetase, class II (G/ P/ S/T) / tRNA synthetase class II core domain (G, H, P, S and T) / Glutathione S-transferase, C-terminal domain / Anticodon-binding / Anticodon binding domain / Anticodon-binding domain superfamily / Glutathione S-transferase, C-terminal domain / Aminoacyl-tRNA synthetase, class I, conserved site / Aminoacyl-transfer RNA synthetases class-I signature. / Aminoacyl-tRNA synthetase, class II / Aminoacyl-transfer RNA synthetases class-II family profile. / Glutathione S-transferase, C-terminal / Glutathione S-transferase Yfyf (Class Pi); Chain A, domain 2 / Class II Aminoacyl-tRNA synthetase/Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL) / Glutathione S-transferase, C-terminal domain superfamily / Rossmann-like alpha/beta/alpha sandwich fold / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / S15/NS1, RNA-binding / Up-down Bundle / Mainly Alpha
Similarity search - Domain/homology
Bifunctional glutamate/proline--tRNA ligase / Aminoacyl tRNA synthase complex-interacting multifunctional protein 2
Similarity search - Component
Biological speciesHOMO SAPIENS (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.318 Å
AuthorsCho, H.Y. / Kang, B.S.
CitationJournal: To be Published
Title: The Crystal Structure of the Gst-Like Domains Complex of Eprs C92Sc105Sc123S Mutant-Aimp2
Authors: Cho, H.Y. / Kang, B.S.
History
DepositionJun 18, 2015Deposition site: PDBE / Processing site: PDBE
Revision 1.0Jun 29, 2016Provider: repository / Type: Initial release
Revision 1.1Jan 10, 2024Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Other / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_database_status / pdbx_initial_refinement_model / struct_conn
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.status_code_sf / _struct_conn.pdbx_leaving_atom_flag
Revision 1.2Oct 16, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: BIFUNCTIONAL GLUTAMATE/PROLINE--TRNA LIGASE
B: AMINOACYL TRNA SYNTHASE COMPLEX-INTERACTING MULTIFUNCTIONA L PROTEIN 2
C: BIFUNCTIONAL GLUTAMATE/PROLINE--TRNA LIGASE
D: AMINOACYL TRNA SYNTHASE COMPLEX-INTERACTING MULTIFUNCTIONA L PROTEIN 2
E: BIFUNCTIONAL GLUTAMATE/PROLINE--TRNA LIGASE
F: AMINOACYL TRNA SYNTHASE COMPLEX-INTERACTING MULTIFUNCTIONA L PROTEIN 2
G: BIFUNCTIONAL GLUTAMATE/PROLINE--TRNA LIGASE
H: AMINOACYL TRNA SYNTHASE COMPLEX-INTERACTING MULTIFUNCTIONA L PROTEIN 2


Theoretical massNumber of molelcules
Total (without water)184,7828
Polymers184,7828
Non-polymers00
Water7,368409
1
A: BIFUNCTIONAL GLUTAMATE/PROLINE--TRNA LIGASE
B: AMINOACYL TRNA SYNTHASE COMPLEX-INTERACTING MULTIFUNCTIONA L PROTEIN 2


Theoretical massNumber of molelcules
Total (without water)46,1952
Polymers46,1952
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2190 Å2
ΔGint-13.2 kcal/mol
Surface area17640 Å2
MethodPISA
2
G: BIFUNCTIONAL GLUTAMATE/PROLINE--TRNA LIGASE
H: AMINOACYL TRNA SYNTHASE COMPLEX-INTERACTING MULTIFUNCTIONA L PROTEIN 2


Theoretical massNumber of molelcules
Total (without water)46,1952
Polymers46,1952
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2050 Å2
ΔGint-11.5 kcal/mol
Surface area17030 Å2
MethodPISA
3
C: BIFUNCTIONAL GLUTAMATE/PROLINE--TRNA LIGASE
D: AMINOACYL TRNA SYNTHASE COMPLEX-INTERACTING MULTIFUNCTIONA L PROTEIN 2


Theoretical massNumber of molelcules
Total (without water)46,1952
Polymers46,1952
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2040 Å2
ΔGint-10.4 kcal/mol
Surface area17940 Å2
MethodPISA
4
E: BIFUNCTIONAL GLUTAMATE/PROLINE--TRNA LIGASE
F: AMINOACYL TRNA SYNTHASE COMPLEX-INTERACTING MULTIFUNCTIONA L PROTEIN 2


Theoretical massNumber of molelcules
Total (without water)46,1952
Polymers46,1952
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1950 Å2
ΔGint-10.6 kcal/mol
Surface area17070 Å2
MethodPISA
Unit cell
Length a, b, c (Å)94.687, 112.793, 181.249
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number18
Space group name H-MP22121

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Components

#1: Protein
BIFUNCTIONAL GLUTAMATE/PROLINE--TRNA LIGASE / BIFUNCTIONAL AMINOACYL-TRNA SYNTHETASE / CELL PROLIFERATION- INDUCING GENE 32 PROTEIN / GLUTAMATYL- ...BIFUNCTIONAL AMINOACYL-TRNA SYNTHETASE / CELL PROLIFERATION- INDUCING GENE 32 PROTEIN / GLUTAMATYL-PROLYL-TRNA SYNTHETASE / GLUTAMYL-TRNA SYNTHETASE / GLURS / PROLYL-TRNA SYNTHETASE / GLUTAMYL- PROLYL-TRNA SYNTHETASE


Mass: 19404.488 Da / Num. of mol.: 4 / Fragment: GST-LIKE DOMAIN / Mutation: YES [C92S, C105S, C123S]
Source method: isolated from a genetically manipulated source
Details: M1 TO R175 OF EPRS GST-LIKE DOMAIN / Source: (gene. exp.) HOMO SAPIENS (human) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): B / Variant (production host): B834(DE3)
References: UniProt: P07814, proline-tRNA ligase, glutamate-tRNA ligase
#2: Protein
AMINOACYL TRNA SYNTHASE COMPLEX-INTERACTING MULTIFUNCTIONA L PROTEIN 2 / MULTISYNTHASE COMPLEX AUXILIARY COMPONENT P38 / PROTEIN JTV- 1


Mass: 26790.916 Da / Num. of mol.: 4 / Fragment: GST-LIKE DOMAIN
Source method: isolated from a genetically manipulated source
Details: T90 TO K320 OF AIMP2 GST-LIKE DOMAIN / Source: (gene. exp.) HOMO SAPIENS (human) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q13155
#3: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 409 / Source method: isolated from a natural source / Formula: H2O
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.59 Å3/Da / Density % sol: 52.6 % / Description: NONE
Crystal growpH: 7.5 / Details: 20% PEG3350, 0.2 M SODIUM FORMATE, pH 7.5

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: PAL/PLS / Beamline: 5C (4A) / Wavelength: 0.9796
DetectorType: ADSC CCD / Detector: CCD / Date: Oct 12, 2014 / Details: VERTICAL FOCUSING TOROIDAL
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9796 Å / Relative weight: 1
ReflectionResolution: 2.2→50 Å / Num. obs: 98969 / % possible obs: 99.5 % / Observed criterion σ(I): -2 / Redundancy: 6.9 % / Biso Wilson estimate: 41.34 Å2 / Rmerge(I) obs: 0.08 / Net I/σ(I): 31.4
Reflection shellResolution: 2.2→2.28 Å / Redundancy: 4.7 % / Rmerge(I) obs: 0.6 / Mean I/σ(I) obs: 2.8 / % possible all: 98.3

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Processing

Software
NameVersionClassification
PHENIX(PHENIX.REFINE)refinement
HKL-2000data reduction
HKL-2000data scaling
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 5A34

5a34


Resolution: 2.318→37.799 Å / SU ML: 0.26 / σ(F): 1.34 / Phase error: 22.71 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.227 3826 2.4 %
Rwork0.1723 --
obs0.1736 161004 98.97 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 2.318→37.799 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms11346 0 0 409 11755
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00811587
X-RAY DIFFRACTIONf_angle_d1.04215779
X-RAY DIFFRACTIONf_dihedral_angle_d14.3644035
X-RAY DIFFRACTIONf_chiral_restr0.0421861
X-RAY DIFFRACTIONf_plane_restr0.0051992
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.3183-2.34760.26911320.22435336X-RAY DIFFRACTION91
2.3476-2.37850.28821430.2195893X-RAY DIFFRACTION100
2.3785-2.41110.24161420.21255864X-RAY DIFFRACTION100
2.4111-2.44550.29121410.21245878X-RAY DIFFRACTION100
2.4455-2.4820.26341470.20695894X-RAY DIFFRACTION100
2.482-2.52080.26731390.19665896X-RAY DIFFRACTION100
2.5208-2.56210.251440.19725918X-RAY DIFFRACTION100
2.5621-2.60630.27051430.19635808X-RAY DIFFRACTION100
2.6063-2.65370.27241430.19475904X-RAY DIFFRACTION100
2.6537-2.70470.24651440.19325883X-RAY DIFFRACTION100
2.7047-2.75990.23941440.19415880X-RAY DIFFRACTION100
2.7599-2.81990.24161450.19335915X-RAY DIFFRACTION100
2.8199-2.88540.26041470.19355882X-RAY DIFFRACTION100
2.8854-2.95760.21281420.19325851X-RAY DIFFRACTION100
2.9576-3.03750.271440.19325878X-RAY DIFFRACTION100
3.0375-3.12680.19671430.19265881X-RAY DIFFRACTION100
3.1268-3.22770.26231450.19565844X-RAY DIFFRACTION100
3.2277-3.3430.25161440.1865926X-RAY DIFFRACTION100
3.343-3.47680.27281410.18335831X-RAY DIFFRACTION100
3.4768-3.63490.18061430.175913X-RAY DIFFRACTION100
3.6349-3.82640.22791420.16245842X-RAY DIFFRACTION100
3.8264-4.06580.18271410.1545867X-RAY DIFFRACTION100
4.0658-4.37930.2031460.14545818X-RAY DIFFRACTION99
4.3793-4.81920.22181360.13195724X-RAY DIFFRACTION98
4.8192-5.51480.16971390.1445816X-RAY DIFFRACTION98
5.5148-6.9410.20541430.16925842X-RAY DIFFRACTION99
6.941-37.80390.2421230.15915194X-RAY DIFFRACTION88
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
17.82082.89372.18946.46710.7336.05230.6609-0.4864-0.48790.5322-0.7253-0.77270.34750.45870.05950.35840.0003-0.04370.45610.19850.418611.4481-3.6513-20.923
26.58341.01221.01038.02770.74259.1459-0.08290.1806-0.72380.1144-0.1627-0.61710.31851.60220.22370.33570.084-0.07630.71190.13420.63517.2648-2.9895-26.1298
38.24461.05131.57211.5042-0.67555.86630.03460.5192-0.6963-0.3886-0.1511-0.27560.55360.64540.10860.38770.05910.04790.31870.04260.3995.7651-6.5215-32.916
45.5275-0.995-2.16015.1232.50677.42510.010.23630.2451-0.1652-0.11250.34780.173-0.02010.13140.27640.0146-0.04340.25830.0770.3352-4.1494-0.0866-33.8592
55.9491-0.3286-0.59934.4126-1.38425.35960.0054-0.14640.36450.39250.0550.1992-0.2376-0.3273-0.06770.3319-0.00310.01310.25490.01940.2802-7.9550.4915-23.9366
66.26790.284-1.36777.0732.03747.61160.085-3.13261.62441.9544-0.06310.5871-0.2635-0.1473-0.08930.73240.01750.0810.8201-0.23010.6463-9.76694.5535-10.9542
78.4276-3.3227-1.02242.2491-1.31983.5175-0.2533-0.3461-0.64120.94270.08740.6071-0.2392-0.03770.38940.3928-0.04820.04190.27890.05150.3486-10.1578-5.8046-17.465
84.23314.66094.11126.03472.51978.7365-0.4422-0.39030.45150.594-0.30030.0867-0.28410.01270.63780.47690.0855-0.13440.35660.05130.48224.547-7.6757-12.49
98.4386-2.35290.29470.6536-0.09578.44250.12410.26160.07490.17490.1722-0.05840.74760.0046-0.28930.23770.0205-0.01460.41180.06330.4042-11.74379.3552-45.0235
107.00260.86251.15124.0486.02349.0013-0.01580.55250.2432-0.67820.21050.10630.17450.3044-0.12470.3510.0329-0.01950.53510.09210.2666-3.10359.7683-52.9508
114.1179-2.3019-2.21154.4622.23285.20890.23930.59830.1575-0.485-0.07840.03150.0937-0.1883-0.01860.38620.0335-0.07450.48640.04580.4514-14.444912.4995-53.0436
125.907-2.81821.43481.6551-0.94822.4580.0637-0.0245-0.2424-0.1439-0.14240.250.3695-0.6890.11170.345-0.068-0.02370.4069-0.0170.4255-12.64138.0696-41.1702
138.36221.73871.72273.8834-1.49211.89760.1127-0.16870.44330.1787-0.2436-0.16230.06520.34430.14170.2340.00370.04040.41630.03380.2228.06239.1501-40.3102
148.07622.95021.01262.4693-1.57455.078-0.08320.12130.6716-0.09180.0550.1383-0.205-0.24120.06860.26140.06850.03680.42120.0520.33299.229715.5113-49.5433
157.0896-0.59863.73713.0125-1.10627.40750.3062-0.2575-0.5563-0.3962-0.17910.68150.5886-0.7707-0.09650.44320.0117-0.12090.4081-0.08240.5698-56.7866-5.7238-63.3842
166.36064.4848-4.92419.1443-3.11913.86180.1146-0.2739-0.1103-0.0609-0.4972-0.45980.18950.41750.34930.29470.0616-0.05520.363-0.04760.2991-40.2395-0.2577-58.0756
175.23111.4344-4.52497.64142.02945.37510.09960.39881.161-0.98740.1615-0.2831-0.8704-0.1742-0.26390.56910.0082-0.05490.43520.04740.4464-35.73017.3701-70.0019
186.91810.8207-1.12253.37811.08073.51520.04170.77690.2409-0.7287-0.119-0.0350.17720.42720.06090.53060.1362-0.07870.43760.01360.3995-37.9348-2.961-73.3302
192.9643-2.1591-0.1243.45684.09878.8052-0.22221.1031-0.1671-0.38070.0447-0.01850.34370.04190.05160.5223-0.0545-0.16270.4327-0.07040.5429-51.1149-8.6787-77.9471
203.95751.22230.58786.5841-1.76364.29610.2622-0.2573-0.2352-0.25140.1265-0.16290.9064-0.1507-0.3580.5448-0.036-0.12190.5601-0.03280.4328-33.32725.3616-46.4834
215.88961.58631.89324.9909-0.63254.36930.3967-1.4794-0.41550.4416-0.3568-0.03950.4158-0.5194-0.04670.3185-0.0404-0.05020.6123-0.01240.3527-35.7119.241-38.8663
229.6695-0.9060.68822.48373.1927.841-0.4257-1.0810.50980.0217-0.0169-0.2274-0.44010.71690.12660.4457-0.0075-0.08460.6003-0.0380.5499-28.552720.8679-44.0623
234.10973.0342.41954.5455.95138.99060.4537-1.6073-0.24972.38640.9612-1.71320.22561.2183-0.85080.99430.1155-0.34921.46950.02820.9501-27.44285.9313-30.5142
244.14242.38242.09392.94691.14846.0155-0.0314-0.0254-0.2108-0.152-0.1714-0.37180.15440.9333-0.0010.29660.0455-0.03790.43090.01330.4172-31.092611.3036-50.6491
257.2854-0.38161.77644.3817-1.45817.17720.35120.2285-1.00630.5421-0.1236-1.33191.38821.1676-0.03380.5150.2412-0.17370.6049-0.03890.6912-31.67670.7346-54.4854
267.29530.98851.70441.70751.88372.40720.1813-0.2474-0.04860.0423-0.12060.14520.164-0.366-0.04760.2570.048-0.02990.438-0.00810.3239-48.58852.9053-49.3133
272.5306-1.15380.38654.9692-1.70515.23270.11440.45460.807-0.21070.34470.2033-0.2027-0.2904-0.50360.30740.02030.00460.60790.05790.6147-57.951216.933-54.4847
283.29270.34810.42213.3086-0.10834.3589-0.0213-0.62530.9890.36970.18680.5336-0.29710.0272-0.17720.27670.05980.02790.5461-0.04740.6161-55.121914.9905-44.0748
291.94881.05861.66868.0791-1.26437.06720.215-1.40651.25450.8403-0.3-0.2157-1.18620.7568-0.02160.5241-0.0112-0.05550.8601-0.27190.7066-43.587919.2846-37.418
302.91570.26480.59453.24242.12246.86480.01210.0085-0.34960.2794-0.0663-0.11930.57750.12670.03970.3086-0.00980.04010.2860.02030.3194-15.2327-38.004-19.4427
314.5113-3.02570.01697.065-2.97236.77930.10720.02550.1270.30130.12310.6516-0.2252-0.5885-0.23760.3285-0.01740.12660.34440.0010.4155-25.9446-20.5757-13.6888
324.309-0.1335-0.96028.0089-0.16092.33310.1220.46860.2384-0.1935-0.08110.5207-0.3465-0.1073-0.01340.2938-0.01620.0510.2940.02580.251-21.4524-17.673-23.5459
333.52053.3651.43985.46354.22464.1723-0.04530.34190.4023-0.6068-0.14210.0498-0.45780.10540.23650.3702-0.02540.06340.3333-0.02720.329-15.0064-28.1599-32.7185
349.080.8343-4.59633.69413.98527.70460.56220.68770.61931.40070.437-1.404-0.63171.1034-0.89751.2129-0.42760.021.2777-0.28450.9981-10.4363-22.23265.9326
359.8936-2.6523-1.91875.1346-2.63494.87720.4150.12920.42430.3173-0.00760.3285-0.7043-0.2579-0.45080.64780.01540.21320.3739-0.06460.4251-32.3533-20.66923.7261
364.84612.0091.69343.50542.18186.70420.6007-0.44920.26571.225-0.1471-0.1425-0.1319-0.1938-0.41280.7854-0.09480.1240.3918-0.07880.3775-25.48-25.186212.1571
373.18150.5354-2.42339.1449-5.48274.69490.4-0.32120.72111.03080.3040.8092-1.5178-1.5801-0.75710.98830.13270.28540.9626-0.10950.7149-39.1393-18.274211.0543
382.0144-2.0395-1.3393.43463.38993.62650.2823-1.32860.92581.25040.7714-0.0663-0.074-1.3179-1.21211.1604-0.18780.12981.0322-0.1270.7901-18.5775-16.395621.2423
394.5017-1.3352-0.97933.4555-0.10023.89820.2029-0.00710.48960.2676-0.003-0.0757-0.4801-0.1997-0.20460.4992-0.09930.11080.2899-0.05140.3601-24.4116-23.53781.0234
404.7340.8424-1.59466.0330.15613.03520.04850.3140.084-0.4379-0.07030.6597-0.1003-0.33060.02550.4797-0.0165-0.04060.320.01450.3218-29.564-42.1202-5.9693
417.1074-2.4144-0.61889.48120.58365.75030.1378-0.3861-0.04380.1805-0.15310.0638-0.1843-0.21350.06510.3825-0.08940.01370.2595-0.00370.2364-27.1334-41.12933.4671
428.35664.93230.64825.0793.26493.9561-0.0583-0.04560.53710.2975-0.25411.493-0.3465-0.76870.38890.42390.03360.04420.432-0.0070.5976-34.929-47.14645.1565
437.1301-2.91891.13575.47731.52227.0016-0.208-1.02490.012.10690.29181.2506-0.4723-0.8534-0.1280.9691-0.1010.38450.5324-0.02260.5881-33.0889-34.638514.0942
445.8671-0.30921.60688.0198-0.47056.1308-0.0738-0.4155-0.11240.1659-0.1105-0.43780.0167-0.22270.19510.48550.1210.09360.55610.06580.4013-26.0093-35.4799-66.2707
453.2630.14485.43699.30051.73149.6870.7952-0.11010.54721.2244-0.96030.1160.5955-1.09590.03760.5543-0.00390.18570.68930.06780.5689-32.2606-38.9707-64.6216
464.572-0.13461.48186.5177-1.15355.62250.2448-0.0366-0.3758-0.7487-0.5188-0.20790.5901-0.13270.27150.6560.18740.10840.55820.00610.4307-29.0182-37.8298-76.0362
473.57222.9236-0.88837.37912.40965.19910.18080.1734-0.1245-1.339-0.2984-1.0193-0.30760.25390.03530.81030.30970.2910.57440.14130.6349-20.2617-19.9983-80.2069
480.88510.8948-1.20358.59421.43432.71890.20580.04560.0581-0.3875-0.3435-0.89-0.10060.35920.11080.45950.22250.07060.5730.08940.5359-22.875-16.4624-69.9279
491.2232-1.0569-1.81613.5766-0.83086.566-0.3532-0.41990.24011.73320.3616-0.6215-0.2495-0.113-0.00750.72150.1317-0.02380.61030.12890.474-26.0412-26.3404-58.4102
500.99510.2845-0.24772.96030.913.13330.35460.53750.4508-1.8781-0.1311-0.0349-1.6264-0.4706-0.22291.8510.46860.37980.79840.16720.6407-23.0695-23.7999-99.5261
513.8316-2.5508-2.51785.36762.89026.7933-0.03320.0729-0.16150.0386-0.0508-0.2843-0.5532-0.23110.08250.64010.09360.11450.3973-0.05260.4297-16.8661-42.1777-93.5833
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1CHAIN 'E' AND (RESID 3 THROUGH 24 )
2X-RAY DIFFRACTION2CHAIN 'E' AND (RESID 25 THROUGH 50 )
3X-RAY DIFFRACTION3CHAIN 'E' AND (RESID 51 THROUGH 71 )
4X-RAY DIFFRACTION4CHAIN 'E' AND (RESID 72 THROUGH 86 )
5X-RAY DIFFRACTION5CHAIN 'E' AND (RESID 87 THROUGH 129 )
6X-RAY DIFFRACTION6CHAIN 'E' AND (RESID 130 THROUGH 144 )
7X-RAY DIFFRACTION7CHAIN 'E' AND (RESID 145 THROUGH 155 )
8X-RAY DIFFRACTION8CHAIN 'E' AND (RESID 156 THROUGH 170 )
9X-RAY DIFFRACTION9CHAIN 'F' AND (RESID 117 THROUGH 132 )
10X-RAY DIFFRACTION10CHAIN 'F' AND (RESID 133 THROUGH 144 )
11X-RAY DIFFRACTION11CHAIN 'F' AND (RESID 145 THROUGH 182 )
12X-RAY DIFFRACTION12CHAIN 'F' AND (RESID 183 THROUGH 209 )
13X-RAY DIFFRACTION13CHAIN 'F' AND (RESID 210 THROUGH 264 )
14X-RAY DIFFRACTION14CHAIN 'F' AND (RESID 265 THROUGH 320 )
15X-RAY DIFFRACTION15CHAIN 'A' AND (RESID 3 THROUGH 71 )
16X-RAY DIFFRACTION16CHAIN 'A' AND (RESID 72 THROUGH 86 )
17X-RAY DIFFRACTION17CHAIN 'A' AND (RESID 87 THROUGH 106 )
18X-RAY DIFFRACTION18CHAIN 'A' AND (RESID 107 THROUGH 156 )
19X-RAY DIFFRACTION19CHAIN 'A' AND (RESID 157 THROUGH 170 )
20X-RAY DIFFRACTION20CHAIN 'B' AND (RESID 112 THROUGH 132 )
21X-RAY DIFFRACTION21CHAIN 'B' AND (RESID 133 THROUGH 154 )
22X-RAY DIFFRACTION22CHAIN 'B' AND (RESID 155 THROUGH 171 )
23X-RAY DIFFRACTION23CHAIN 'B' AND (RESID 172 THROUGH 182 )
24X-RAY DIFFRACTION24CHAIN 'B' AND (RESID 183 THROUGH 198 )
25X-RAY DIFFRACTION25CHAIN 'B' AND (RESID 199 THROUGH 209 )
26X-RAY DIFFRACTION26CHAIN 'B' AND (RESID 210 THROUGH 242 )
27X-RAY DIFFRACTION27CHAIN 'B' AND (RESID 243 THROUGH 264 )
28X-RAY DIFFRACTION28CHAIN 'B' AND (RESID 265 THROUGH 307 )
29X-RAY DIFFRACTION29CHAIN 'B' AND (RESID 308 THROUGH 321 )
30X-RAY DIFFRACTION30CHAIN 'C' AND (RESID 3 THROUGH 71 )
31X-RAY DIFFRACTION31CHAIN 'C' AND (RESID 72 THROUGH 106 )
32X-RAY DIFFRACTION32CHAIN 'C' AND (RESID 107 THROUGH 155 )
33X-RAY DIFFRACTION33CHAIN 'C' AND (RESID 156 THROUGH 170 )
34X-RAY DIFFRACTION34CHAIN 'D' AND (RESID 105 THROUGH 120 )
35X-RAY DIFFRACTION35CHAIN 'D' AND (RESID 121 THROUGH 132 )
36X-RAY DIFFRACTION36CHAIN 'D' AND (RESID 133 THROUGH 154 )
37X-RAY DIFFRACTION37CHAIN 'D' AND (RESID 155 THROUGH 171 )
38X-RAY DIFFRACTION38CHAIN 'D' AND (RESID 172 THROUGH 182 )
39X-RAY DIFFRACTION39CHAIN 'D' AND (RESID 183 THROUGH 226 )
40X-RAY DIFFRACTION40CHAIN 'D' AND (RESID 227 THROUGH 264 )
41X-RAY DIFFRACTION41CHAIN 'D' AND (RESID 265 THROUGH 288 )
42X-RAY DIFFRACTION42CHAIN 'D' AND (RESID 289 THROUGH 307 )
43X-RAY DIFFRACTION43CHAIN 'D' AND (RESID 308 THROUGH 320 )
44X-RAY DIFFRACTION44CHAIN 'G' AND (RESID 3 THROUGH 24 )
45X-RAY DIFFRACTION45CHAIN 'G' AND (RESID 25 THROUGH 35 )
46X-RAY DIFFRACTION46CHAIN 'G' AND (RESID 36 THROUGH 71 )
47X-RAY DIFFRACTION47CHAIN 'G' AND (RESID 72 THROUGH 106 )
48X-RAY DIFFRACTION48CHAIN 'G' AND (RESID 107 THROUGH 155 )
49X-RAY DIFFRACTION49CHAIN 'G' AND (RESID 156 THROUGH 171 )
50X-RAY DIFFRACTION50CHAIN 'H' AND (RESID 120 THROUGH 226 )
51X-RAY DIFFRACTION51CHAIN 'H' AND (RESID 227 THROUGH 319 )

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