+Open data
-Basic information
Entry | Database: PDB / ID: 5a43 | ||||||
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Title | Crystal structure of a dual topology fluoride ion channel. | ||||||
Components |
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Keywords | TRANSPORT PROTEIN / FLUORIDE ION CHANNEL / FLUC / EC2 | ||||||
Function / homology | Function and homology information inorganic anion transmembrane transporter activity / plasma membrane => GO:0005886 Similarity search - Function | ||||||
Biological species | ESCHERICHIA COLI S88 (bacteria) HOMO SAPIENS (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2.58 Å | ||||||
Authors | Stockbridge, R.B. / Kolmakova-Partensky, L. / Shane, T. / Koide, A. / Koide, S. / Miller, C. / Newstead, S. | ||||||
Citation | Journal: Nature / Year: 2015 Title: Crystal Structures of a Double-Barrelled Fluoride Ion Channel. Authors: Stockbridge, R.B. / Kolmakova-Partensky, L. / Shane, T. / Koide, A. / Koide, S. / Miller, C. / Newstead, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5a43.cif.gz | 93.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5a43.ent.gz | 78 KB | Display | PDB format |
PDBx/mmJSON format | 5a43.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 5a43_validation.pdf.gz | 575.6 KB | Display | wwPDB validaton report |
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Full document | 5a43_full_validation.pdf.gz | 582.5 KB | Display | |
Data in XML | 5a43_validation.xml.gz | 11.5 KB | Display | |
Data in CIF | 5a43_validation.cif.gz | 17.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a4/5a43 ftp://data.pdbj.org/pub/pdb/validation_reports/a4/5a43 | HTTPS FTP |
-Related structure data
Related structure data | 5a40C 5nkqC 5a41 C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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-Components
-Protein , 2 types, 4 molecules ABCD
#1: Protein | Mass: 13863.006 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ESCHERICHIA COLI S88 (bacteria) / Variant: O45\:K1 / Plasmid: PASK-IBA2 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: B7LI20 #2: Protein | Mass: 10365.372 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Plasmid: PHFT2 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) |
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-Sugars , 1 types, 3 molecules
#4: Sugar |
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-Non-polymers , 3 types, 22 molecules
#3: Chemical | #5: Chemical | ChemComp-NA / | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 6.03 Å3/Da / Density % sol: 79 % / Description: NONE |
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Crystal grow | pH: 6 / Details: 29% PEG550 MME, 50 MM LINO3, 100 MM ADA PH 6.0 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 8.2.2 / Wavelength: 0.979 |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Nov 20, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→25 Å / Num. obs: 34593 / % possible obs: 99.9 % / Observed criterion σ(I): 2 / Redundancy: 14.3 % / Biso Wilson estimate: 62.65 Å2 / Rmerge(I) obs: 0.17 / Net I/σ(I): 9 |
Reflection shell | Resolution: 2.6→2.7 Å / Redundancy: 14.3 % / Rmerge(I) obs: 0.99 / Mean I/σ(I) obs: 1.9 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: SAD Starting model: NONE Resolution: 2.58→24.246 Å / SU ML: 0.45 / σ(F): 1.36 / Phase error: 32.67 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 62.65 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.58→24.246 Å
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Refine LS restraints |
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LS refinement shell |
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