PDB-4zp1: Crystal structure of Zymomonas mobilis pyruvate decarboxylase var...

ID or keywords:

Entry

Database: PDB / ID: 4zp1

Title

Crystal structure of Zymomonas mobilis pyruvate decarboxylase variant Glu473Ala

Components

Pyruvate decarboxylase

Keywords

LYASE / thiamin diphosphate (ThDP) / pyruvate decarboxylase

Function / homology

Function and homology information

pyruvate decarboxylase / : / pyruvate decarboxylase activity / thiamine pyrophosphate binding / magnesium ion binding / cytosol
Similarity search - Function

Biological species

Zymomonas mobilis (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

FOURIER SYNTHESIS / Resolution: 2.205 Å

Authors

Wechsler, C. / Neumann, P. / Tittmann, K.

Funding support

Germany, 1items

Citation

Journal: Chembiochem / Year: 2015
Title: Tuning and Switching Enantioselectivity of Asymmetric Carboligation in an Enzyme through Mutational Analysis of a Single Hot Spot.
Authors: Wechsler, C. / Meyer, D. / Loschonsky, S. / Funk, L.M. / Neumann, P. / Ficner, R. / Brodhun, F. / Muller, M. / Tittmann, K.

History

Deposition	May 7, 2015	Deposition site: RCSB / Processing site: PDBE
Revision 1.0	Nov 4, 2015	Provider: repository / Type: Initial release
Revision 1.1	Jan 27, 2016	Group: Database references
Revision 1.2	Sep 6, 2017	Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.3	May 8, 2024	Group: Data collection / Database references / Derived calculations Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / diffrn_source / pdbx_struct_conn_angle / struct_conn Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _diffrn_source.pdbx_synchrotron_beamline / _diffrn_source.pdbx_synchrotron_site / _diffrn_source.type / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_ptnr1_label_alt_id / _struct_conn.pdbx_ptnr2_label_alt_id / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	4zp1.cif.gz	867.2 KB	Display	PDBx/mmCIF format
PDB format	pdb4zp1.ent.gz	723.3 KB	Display	PDB format
PDBx/mmJSON format	4zp1.json.gz		Tree view	PDBx/mmJSON format
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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/zp/4zp1 ftp://data.pdbj.org/pub/pdb/validation_reports/zp/4zp1	HTTPS FTP

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Related structure data

Similar structure data	5euj-2 1zpd-d 5euj-1 5euj-5 5euj-4 5euj-6 5euj-3 2vbi-2 2wvh-2 2wvh-1 Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#263 - Nov 2021 Acetohydroxyacid Synthase similarity (11) #213 - Sep 2017 Sirtuins similarity (1) #153 - Sep 2012 Pyruvate Dehydrogenase Complex similarity (2) #154 - Oct 2012 Citric Acid Cycle similarity (1)

Deposited unit

A: Pyruvate decarboxylase

B: Pyruvate decarboxylase

C: Pyruvate decarboxylase

D: Pyruvate decarboxylase

hetero molecules

246 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by author&software

Unit cell

Length a, b, c (Å)	70.290, 167.640, 110.820
Angle α, β, γ (deg.)	90.00, 101.66, 90.00
Int Tables number	4
Space group name H-M	P12₁1

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Protein , 1 types, 4 molecules ABCD

#1: Protein	Pyruvate decarboxylase / PDC Mass: 60932.289 Da / Num. of mol.: 4 / Mutation: E473A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Zymomonas mobilis (bacteria) / Gene: pdc, ZMO1360 / Production host: Escherichia coli (E. coli) / References: UniProt: P06672, pyruvate decarboxylase

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Non-polymers , 5 types, 619 molecules

#2: Chemical	ChemComp-MG / MAGNESIUM ION Mass: 24.305 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Mg
#3: Chemical	ChemComp-NI / NICKEL (II) ION Mass: 58.693 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Ni
#4: Chemical	ChemComp-TPP / THIAMINE DIPHOSPHATE Mass: 425.314 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C₁₂H₁₉N₄O₇P₂S
#5: Chemical	ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL Mass: 92.094 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C₃H₈O₃
#6: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 607 / Source method: isolated from a natural source / Formula: H₂O

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.62 Å³/Da / Density % sol: 53.12 %
Crystal grow	Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 6 Details: ZmPDC variant Glu473Ala was concentrated to 7.5 mg/mL in 10 mM citrate buffer (pH 6.0) containing 1 mM ThDP and 1 mM MgSO4. 1 microL of the enzyme was mixed with 1 microL of the reservoir ...Details: ZmPDC variant Glu473Ala was concentrated to 7.5 mg/mL in 10 mM citrate buffer (pH 6.0) containing 1 mM ThDP and 1 mM MgSO4. 1 microL of the enzyme was mixed with 1 microL of the reservoir solution containing 100 mM Tris, 40 mM NiCl2, 15% PEG2000, 1 mM ThDP, 1 mM MgSO4, and 100 mM sodium malonate.

Crystal

Density Matthews: 2.62 Å³/Da / Density % sol: 53.12 %

Crystal grow

Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 6
Details: ZmPDC variant Glu473Ala was concentrated to 7.5 mg/mL in 10 mM citrate buffer (pH 6.0) containing 1 mM ThDP and 1 mM MgSO4. 1 microL of the enzyme was mixed with 1 microL of the reservoir ...

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Data collection

Diffraction

Mean temperature: 100 K

Diffraction source

Source:

SYNCHROTRON / Site:

PETRA III, EMBL c/o DESY

/ Beamline: P13 (MX1) / Wavelength: 0.9842 Å

Detector

Type: PSI PILATUS 6M / Detector: PIXEL / Date: Apr 3, 2014

Radiation

Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray

Radiation wavelength

Wavelength: 0.9842 Å / Relative weight: 1

Reflection

Resolution: 2.2→47.55 Å / Num. all: 124039 / Num. obs: 124039 / % possible obs: 98.3 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 3.8 % / B_iso Wilson estimate: 37.43 Å² / R_merge F obs: 0.999 / R_merge(I) obs: 0.061 / R_rim(I) all: 0.071 / Χ²: 1.009 / Net I/σ(I): 16.15 / Num. measured all: 471480

Reflection shell

Diffraction-ID: 1 / Rejects: _

Resolution (Å)	Highest resolution (Å)	Redundancy (%)	R_merge F obs	R_merge(I) obs	Mean I/σ(I) obs	Num. measured obs	Num. possible	Num. unique obs	R_rim(I) all	% possible all
2.2-2.31		3.7	0.803	0.559	2.38	55638	15666	15208	0.656	97.1
2.31-2.4			0.894	0.434	3.19	51433	13149	13029	0.502	99.1
2.4-2.61			0.937	0.3	4.45	79145	20708	20464	0.348	98.8
2.61-3.38			0.993	0.105	11.13	156271	41599	40905	0.122	98.3
3.38-3.78			0.998	0.042	26.2	34650	9725	9467	0.049	97.3
3.78-4.16			0.999	0.031	36.87	25301	6443	6396	0.036	99.3
4.16-4.55			0.999	0.025	43.51	16897	4440	4407	0.029	99.3
4.55-14			0.999	0.021	49	50546	13958	13683	0.024	98
14-17			0.999	0.014	78.43	839	225	223	0.016	99.1
17-50			0.999	0.015	62.24	760	280	257	0.018	91.8
	50						16

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Processing

Software

Name	Version	Classification
PHENIX	1.9_1692	refinement
XSCALE		data scaling
PDB_EXTRACT	3.15	data extraction
XDS		data reduction
PHASER		phasing

Refinement

Method to determine structure:

FOURIER SYNTHESIS / Resolution: 2.205→47.545 Å / SU ML: 0.26 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 25.69 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection	Selection details
R_free	0.2166	6195	5 %	Random selection
R_work	0.1698	-	-	-
obs	0.1721	123937	98.26 %	-

Solvent computation

Shrinkage radii: 1 Å / VDW probe radii: 1.2 Å / Solvent model: FLAT BULK SOLVENT MODEL

Refinement step

Cycle: LAST / Resolution: 2.205→47.545 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	17048	0	122	607	17777

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.005	17645
X-RAY DIFFRACTION	f_angle_d	0.844	24017
X-RAY DIFFRACTION	f_dihedral_angle_d	12.441	6321
X-RAY DIFFRACTION	f_chiral_restr	0.032	2670
X-RAY DIFFRACTION	f_plane_restr	0.003	3127

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
2.205-2.2301	0.3448	202	0.2936	3832	X-RAY DIFFRACTION	97
2.2301-2.2563	0.3444	201	0.2876	3818	X-RAY DIFFRACTION	95
2.2563-2.2838	0.3169	202	0.2699	3858	X-RAY DIFFRACTION	97
2.2838-2.3127	0.316	207	0.2526	3947	X-RAY DIFFRACTION	99
2.3127-2.3432	0.3041	208	0.2467	3957	X-RAY DIFFRACTION	98
2.3432-2.3753	0.2994	206	0.2411	3917	X-RAY DIFFRACTION	99
2.3753-2.4092	0.2711	207	0.2393	3934	X-RAY DIFFRACTION	99
2.4092-2.4451	0.3082	208	0.2305	3938	X-RAY DIFFRACTION	99
2.4451-2.4834	0.2932	207	0.2198	3946	X-RAY DIFFRACTION	99
2.4834-2.5241	0.2761	209	0.2174	3981	X-RAY DIFFRACTION	99
2.5241-2.5676	0.2689	206	0.2114	3900	X-RAY DIFFRACTION	99
2.5676-2.6143	0.2878	206	0.2014	3925	X-RAY DIFFRACTION	98
2.6143-2.6645	0.2577	207	0.2045	3921	X-RAY DIFFRACTION	98
2.6645-2.7189	0.2785	206	0.2045	3914	X-RAY DIFFRACTION	97
2.7189-2.778	0.2698	198	0.2049	3753	X-RAY DIFFRACTION	95
2.778-2.8427	0.2805	209	0.2149	3964	X-RAY DIFFRACTION	99
2.8427-2.9137	0.2597	208	0.2099	3956	X-RAY DIFFRACTION	99
2.9137-2.9925	0.2658	208	0.1967	3945	X-RAY DIFFRACTION	99
2.9925-3.0806	0.2349	208	0.2019	3957	X-RAY DIFFRACTION	99
3.0806-3.18	0.265	208	0.1937	3955	X-RAY DIFFRACTION	99
3.18-3.2936	0.2462	207	0.1835	3928	X-RAY DIFFRACTION	99
3.2936-3.4254	0.2153	207	0.1754	3934	X-RAY DIFFRACTION	99
3.4254-3.5813	0.2269	204	0.1739	3871	X-RAY DIFFRACTION	97
3.5813-3.77	0.2047	205	0.1484	3896	X-RAY DIFFRACTION	98
3.77-4.0061	0.1731	209	0.1368	3973	X-RAY DIFFRACTION	99
4.0061-4.3152	0.156	209	0.1202	3982	X-RAY DIFFRACTION	99
4.3152-4.7491	0.1441	210	0.1125	3986	X-RAY DIFFRACTION	99
4.7491-5.4355	0.1716	202	0.1279	3868	X-RAY DIFFRACTION	96
5.4355-6.8449	0.1746	211	0.144	4006	X-RAY DIFFRACTION	100
6.8449-47.556	0.1503	210	0.1299	3980	X-RAY DIFFRACTION	98

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	1.6201	0.621	-0.6619	2.0941	-0.1051	0.8783	-0.0147	0.0118	-0.0544	0.2486	0.03	-0.6121	0.0848	0.1741	-0.017	0.358	0.0341	-0.1617	0.3278	-0.0174	0.4757	19.089	0.9601	129.8881
2	0.5634	0.2938	0.0554	1.4563	0.4759	1.4479	0.1826	0.109	-0.0917	-0.1227	0.0713	-0.5549	0.0005	0.1722	-0.244	0.2969	0.0148	-0.0362	0.3408	-0.0346	0.5352	18.9144	1.1757	118.6001
3	1.5027	0.0898	0.5336	0.7714	-0.4405	0.46	-0.1086	0.047	-0.302	0.1976	0.0672	-0.0259	-0.1642	0.0111	0.0414	0.3516	0.0151	-0.0047	0.319	-0.0315	0.3556	5.8282	23.5953	123.6866
4	0.9356	0.1961	-0.032	2.4182	-0.3961	1.659	0.0104	-0.0455	-0.0147	0.2798	0.0853	0.1717	0.0345	-0.2547	-0.0967	0.3318	0.0149	0.0374	0.395	0.023	0.2729	-14.3576	17.0868	131.412
5	0.6321	0.2825	-0.4471	1.6792	-0.3243	0.7372	0.0278	-0.1298	-0.1643	0.5892	0.0503	-0.1182	0.1795	-0.116	-0.0772	0.6668	0.0072	-0.0932	0.426	0.0149	0.2574	-2.4745	-5.9426	146.4155
6	1.7547	0.1804	0.9366	1.5943	0.4341	1.1663	0.0451	0.0402	-0.2282	0.3696	0.0131	0.2054	0.2582	-0.129	-0.0289	0.5036	-0.0805	0.0068	0.4699	0.057	0.2864	-12.5194	-8.3543	136.3121
7	1.4982	0.2604	-0.0393	1.8298	-0.0609	1.8877	-0.0103	-0.0822	-0.1459	0.2346	0.0517	-0.2029	0.2077	-0.1391	-0.032	0.3812	-0.0126	0.0054	0.3085	0.0365	0.2976	-2.8287	-25.3695	121.636
8	0.6096	0.5185	-0.0121	2.1488	0.7024	1.3522	0.0391	0.0502	0.0798	-0.0545	0.027	-0.2057	-0.079	-0.062	-0.0613	0.294	-0.0009	-0.0226	0.2607	0.0101	0.2643	-0.6331	-14.3232	113.9832
9	0.9832	-0.3021	0.2248	0.4462	0.047	0.8028	-0.0389	-0.0582	0.0177	0.1008	0.1711	-0.5982	0.1943	0.1694	-0.1209	0.441	0.0794	-0.0857	0.3861	-0.1182	0.882	29.858	-35.384	109.9911
10	0.2674	-0.0098	-0.2927	0.5317	0.4502	0.9753	0.0627	-0.0749	-0.1883	0.2329	0.1247	-0.4986	0.1769	0.2422	-0.144	0.4817	0.0802	-0.1834	0.3646	-0.0336	0.82	25.512	-30.9954	123.089
11	0.7333	-0.6069	0.6147	2.3646	-0.1447	0.9649	-0.0662	-0.1901	-0.2065	0.4928	0.0384	-0.66	0.1982	0.1406	0.0359	0.7733	0.0479	-0.2405	0.4292	0.0662	0.6607	16.8974	-24.6411	143.7938
12	2.5165	0.722	-1.1417	0.918	-0.0458	1.3437	-0.0147	-0.1376	0.2013	0.2884	0.2324	-0.503	0.0376	0.2802	-0.2103	0.5979	0.0349	-0.3392	0.4839	-0.0247	1.0638	30.0147	-19.5456	132.4691
13	1.4169	-0.2906	0.6967	1.96	-0.0602	0.6898	0.0287	0.0409	0.0291	-0.2487	0.0364	-0.5428	-0.0587	0.1424	-0.066	0.3889	-0.0193	0.1681	0.358	-0.0258	0.4797	19.8279	-6.8397	87.4278
14	0.743	0.1932	0.024	2.1542	0.5046	1.1926	0.1117	-0.169	0.1374	-0.0949	0.0594	-0.7957	-0.0427	0.1112	-0.1688	0.2807	-0.0121	0.0647	0.3003	-0.036	0.5146	19.2454	-7.1202	98.6509
15	0.0624	-0.2234	-0.0705	1.59	-0.3588	0.5178	0.1614	-0.0642	-0.1406	-0.0863	0.0126	-0.0756	0.2319	-0.0936	-0.1624	0.3567	-0.0327	0.0058	0.3574	-0.0706	0.276	0.3587	-29.2975	91.5017
16	1.1581	0.2663	0.1672	2.1506	-0.3592	0.1714	-0.0932	0.0805	-0.0244	-0.2263	0.1322	0.2324	-0.0588	-0.218	-0.0332	0.3579	-0.0004	-0.0256	0.4639	0.0346	0.2538	-13.7967	-19.0312	83.1817
17	2.0889	-0.1963	-0.1268	2.6081	-1.0551	2.1344	0.067	0.1392	-0.2582	-0.1002	-0.0318	0.1887	0.4024	-0.2215	-0.0152	0.3678	-0.0619	0.0102	0.3603	-0.0218	0.3299	-11.2228	-30.2241	87.9035
18	0.7954	-0.1033	0.064	0.956	-0.5744	0.7313	0.0881	0.1548	0.0762	-0.3368	0.0531	0.0417	-0.0335	-0.1758	-0.1513	0.564	-0.005	0.037	0.4417	0.0375	0.2695	-5.7243	-6.8715	70.9055
19	0.3192	-0.6041	0.3872	2.2073	0.3354	1.4405	0.0446	0.2272	0.1446	-0.4545	-0.0538	-0.0314	-0.2157	-0.0514	0.0138	0.5954	-0.0195	0.1503	0.428	0.059	0.3365	3.4125	6.498	72.1839
20	1.6225	0.5758	-1.1164	1.5817	0.5058	1.3982	-0.1681	0.0349	0.2004	-0.5182	0.1092	0.1472	0.0541	-0.0994	0.0441	0.5697	0.1364	0.0225	0.4925	0.1068	0.3193	-8.3591	2.5569	73.0605
21	1.8099	-0.3651	-0.4491	2.18	0.4231	1.5634	-0.0131	0.0689	0.0373	-0.1687	0.011	-0.1527	-0.0915	-0.0977	0.0013	0.2987	0.0013	0.0217	0.2719	0.0145	0.2396	-0.8741	14.8775	96.3275
22	0.7025	-0.9847	-0.1962	2.4084	0.7468	1.1993	-0.0188	0.0167	-0.0712	0.0728	0.0519	-0.1699	0.1776	-0.034	-0.0205	0.2858	-0.0117	0.047	0.2677	0.0122	0.2413	-1.8442	8.6412	104.9324
23	0.4924	0.2374	-0.0892	0.6168	0.2668	1.0856	-0.0972	0.1225	-0.0969	-0.0912	0.0942	-0.4254	-0.0289	0.1574	-0.0021	0.3603	-0.0339	0.0584	0.3878	-0.0506	0.7818	29.5637	27.0676	109.2344
24	0.2056	-0.0325	0.2332	0.4361	0.668	1.3945	-0.0297	0.1764	0.0686	-0.4602	0.1326	-0.7864	-0.673	0.6359	-0.0261	0.5888	-0.1703	0.2661	0.6232	-0.0296	0.9692	39.8269	33.4322	90.9599
25	1.2992	0.1791	0.1932	0.3536	0.2232	1.3865	0.0169	0.1045	0.4635	-0.2997	0.062	-0.3975	-0.2427	0.192	-0.0831	0.5146	-0.0338	0.197	0.3634	0.0183	0.7298	19.5469	23.7824	85.2908
26	0.9108	0.3979	-1.0585	2.443	-0.0187	1.3023	-0.0415	-0.0191	0.2183	-0.4455	0.096	-0.6534	-0.039	0.0603	-0.0443	0.6692	-0.0317	0.2689	0.4251	0.0178	0.5987	17.4538	16.2592	73.945
27	1.4108	0.1624	0.6515	1.7381	-0.3153	1.5606	-0.0295	0.1889	-0.2659	-0.5784	0.2258	-0.4555	-0.0604	0.359	-0.1689	0.6211	-0.0327	0.3425	0.5131	0.0087	0.8568	28.6691	17.6088	81.8789

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 2 through 110 )
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 111 through 170 )
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 171 through 206 )
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 207 through 348 )
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 349 through 527 )
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 528 through 566 )
7	X-RAY DIFFRACTION	7	chain 'B' and (resid 2 through 64 )
8	X-RAY DIFFRACTION	8	chain 'B' and (resid 65 through 180 )
9	X-RAY DIFFRACTION	9	chain 'B' and (resid 181 through 283 )
10	X-RAY DIFFRACTION	10	chain 'B' and (resid 284 through 455 )
11	X-RAY DIFFRACTION	11	chain 'B' and (resid 456 through 527 )
12	X-RAY DIFFRACTION	12	chain 'B' and (resid 528 through 566 )
13	X-RAY DIFFRACTION	13	chain 'C' and (resid 2 through 110 )
14	X-RAY DIFFRACTION	14	chain 'C' and (resid 111 through 170 )
15	X-RAY DIFFRACTION	15	chain 'C' and (resid 171 through 216 )
16	X-RAY DIFFRACTION	16	chain 'C' and (resid 217 through 307 )
17	X-RAY DIFFRACTION	17	chain 'C' and (resid 308 through 347 )
18	X-RAY DIFFRACTION	18	chain 'C' and (resid 348 through 432 )
19	X-RAY DIFFRACTION	19	chain 'C' and (resid 433 through 507 )
20	X-RAY DIFFRACTION	20	chain 'C' and (resid 508 through 566 )
21	X-RAY DIFFRACTION	21	chain 'D' and (resid 2 through 110 )
22	X-RAY DIFFRACTION	22	chain 'D' and (resid 111 through 180 )
23	X-RAY DIFFRACTION	23	chain 'D' and (resid 181 through 334 )
24	X-RAY DIFFRACTION	24	chain 'D' and (resid 335 through 366 )
25	X-RAY DIFFRACTION	25	chain 'D' and (resid 367 through 455 )
26	X-RAY DIFFRACTION	26	chain 'D' and (resid 456 through 514 )
27	X-RAY DIFFRACTION	27	chain 'D' and (resid 515 through 566 )

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