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- PDB-4zgn: Structure Cdc123 complexed with the C-terminal domain of eIF2gamma -
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Open data
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Basic information
Entry | Database: PDB / ID: 4zgn | |||||||||
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Title | Structure Cdc123 complexed with the C-terminal domain of eIF2gamma | |||||||||
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![]() | CELL CYCLE / ATP-grasp fold / eIF2 assembly | |||||||||
Function / homology | ![]() eukaryotic translation initiation factor 2 complex assembly / Cellular response to mitochondrial stress / Recycling of eIF2:GDP / ABC-family proteins mediated transport / methionyl-initiator methionine tRNA binding / eukaryotic translation initiation factor 2 complex / multi-eIF complex / selenocysteine metabolic process / eukaryotic 43S preinitiation complex / protein-synthesizing GTPase ...eukaryotic translation initiation factor 2 complex assembly / Cellular response to mitochondrial stress / Recycling of eIF2:GDP / ABC-family proteins mediated transport / methionyl-initiator methionine tRNA binding / eukaryotic translation initiation factor 2 complex / multi-eIF complex / selenocysteine metabolic process / eukaryotic 43S preinitiation complex / protein-synthesizing GTPase / selenocysteine insertion sequence binding / formation of translation preinitiation complex / eukaryotic 48S preinitiation complex / positive regulation of translational fidelity / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / L13a-mediated translational silencing of Ceruloplasmin expression / protein folding chaperone / translation initiation factor binding / translational initiation / translation initiation factor activity / ribosome / GTPase activity / GTP binding / magnesium ion binding / ATP binding / cytoplasm / cytosol Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Panvert, M. / Dubiez, E. / Arnold, L. / Perez, J. / Seufert, W. / Mechulam, Y. / Schmitt, E. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Cdc123, a Cell Cycle Regulator Needed for eIF2 Assembly, Is an ATP-Grasp Protein with Unique Features. Authors: Panvert, M. / Dubiez, E. / Arnold, L. / Perez, J. / Mechulam, Y. / Seufert, W. / Schmitt, E. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 176.8 KB | Display | ![]() |
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PDB format | ![]() | 139.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 759.7 KB | Display | ![]() |
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Full document | ![]() | 765 KB | Display | |
Data in XML | ![]() | 15.8 KB | Display | |
Data in CIF | ![]() | 20.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4zgoSC ![]() 4zgpC ![]() 4zgqC ![]() 2ahoS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 38979.051 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: cdc123, SPAP27G11.03 / Plasmid: pet15b / Production host: ![]() ![]() |
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#2: Protein | Mass: 13108.387 Da / Num. of mol.: 1 / Fragment: residues 410-527 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: GCD11, TIF213, YER025W / Plasmid: pET3a / Production host: ![]() ![]() |
#3: Chemical | ChemComp-ATP / |
#4: Chemical | ChemComp-MG / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.76 Å3/Da / Density % sol: 55.37 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 8 / Details: 25%PEG3350, 0.2MLiSO4, 0.1M TrispH8.0 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Mar 12, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97857 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→45 Å / Num. obs: 13237 / % possible obs: 99.7 % / Redundancy: 5.8 % / Biso Wilson estimate: 100.9 Å2 / Rsym value: 0.074 / Net I/σ(I): 15.8 |
Reflection shell | Resolution: 2.9→3.07 Å / Redundancy: 5.9 % / Rmerge(I) obs: 0.875 / Mean I/σ(I) obs: 2.2 / % possible all: 99.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4ZGO and 2AHO Resolution: 2.9→45 Å / Cor.coef. Fo:Fc: 0.9191 / Cor.coef. Fo:Fc free: 0.8798 / SU R Cruickshank DPI: 1.337 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 1.203 / SU Rfree Blow DPI: 0.362 / SU Rfree Cruickshank DPI: 0.37
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Displacement parameters | Biso mean: 101.14 Å2
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Refine analyze | Luzzati coordinate error obs: 0.512 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.9→45 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.9→3.13 Å / Total num. of bins used: 7
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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