ジャーナル: Acta Crystallogr.,Sect.D / 年: 2015 タイトル: Structures of yeast peroxisomal Delta (3), Delta (2)-enoyl-CoA isomerase complexed with acyl-CoA substrate analogues: the importance of hydrogen-bond networks for the reactivity of the ...タイトル: Structures of yeast peroxisomal Delta (3), Delta (2)-enoyl-CoA isomerase complexed with acyl-CoA substrate analogues: the importance of hydrogen-bond networks for the reactivity of the catalytic base and the oxyanion hole. 著者: Onwukwe, G.U. / Koski, M.K. / Pihko, P. / Schmitz, W. / Wierenga, R.K.
分子量: 33965.848 Da / 分子数: 3 / 由来タイプ: 組換発現 詳細: CYS212 was oxidized to CME due to the presence of BME in the purification buffers. 由来: (組換発現) Saccharomyces cerevisiae (パン酵母) 遺伝子: ECI1, YLR284C / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: Q05871, Delta3-Delta2-enoyl-CoA isomerase
解像度: 2.13→47.31 Å / Cor.coef. Fo:Fc: 0.968 / Cor.coef. Fo:Fc free: 0.957 / SU B: 3.57 / SU ML: 0.089 / 交差検証法: THROUGHOUT / ESU R: 0.131 / ESU R Free: 0.12 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.19016
4307
5 %
RANDOM
Rwork
0.1681
-
-
-
obs
0.1692
81724
99.9 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK