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Open data
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Basic information
Entry | Database: PDB / ID: 4z4a | ||||||
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Title | Avirulence protein 4 (Avr4) from Pseudocercospora fuligena | ||||||
![]() | Carbohydrate-binding module family 14 protein | ||||||
![]() | SUGAR BINDING PROTEIN / Effector protein / Avirulence protein / CBM-14 | ||||||
Function / homology | Chitin-binding domain type 2 / Chitin binding domain / Chitin binding Peritrophin-A domain / Chitin-binding type-2 domain profile. / Chitin binding domain superfamily / chitin binding / extracellular region / Carbohydrate-binding module family 14 protein![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Hurlburt, N.K. / Kohler, A.C. / Fisher, A.J. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural Analysis of an Avr4 Effector Ortholog Offers Insight into Chitin Binding and Recognition by the Cf-4 Receptor. Authors: Kohler, A.C. / Chen, L.H. / Hurlburt, N. / Salvucci, A. / Schwessinger, B. / Fisher, A.J. / Stergiopoulos, I. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 74.3 KB | Display | ![]() |
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PDB format | ![]() | 58.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 450.2 KB | Display | ![]() |
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Full document | ![]() | 450.9 KB | Display | |
Data in XML | ![]() | 10.4 KB | Display | |
Data in CIF | ![]() | 13.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 13389.963 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Chemical | ChemComp-EDO / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 46.52 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 7.5 / Details: Ammonium sulfate (1.6-2.0M), 0.1M HEPES, pH 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K | |||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Dec 6, 2013 | |||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||
Radiation wavelength |
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Reflection | Resolution: 1.7→50 Å / Num. obs: 26958 / % possible obs: 99 % / Redundancy: 6.7 % / Rmerge(I) obs: 0.052 / Net I/σ(I): 17.9 | |||||||||
Reflection shell | Resolution: 1.7→1.74 Å / Redundancy: 5.6 % / Rmerge(I) obs: 0.671 / Mean I/σ(I) obs: 2.61 / % possible all: 99.6 |
-Phasing
Phasing | Method: ![]() |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 33.234 Å2
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Refinement step | Cycle: LAST / Resolution: 1.7→32.16 Å
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Refine LS restraints |
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