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Yorodumi- PDB-4yhj: Structure and Function of the Hypertension Variant A486V of G Pro... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4yhj | ||||||
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Title | Structure and Function of the Hypertension Variant A486V of G Protein-coupled Receptor Kinase 4 (GRK4) | ||||||
Components | G protein-coupled receptor kinase 4 | ||||||
Keywords | TRANSFERASE | ||||||
Function / homology | Function and homology information G-protein-coupled receptor kinase / rhodopsin kinase activity / G protein-coupled receptor kinase activity / regulation of opsin-mediated signaling pathway / regulation of G protein-coupled receptor signaling pathway / G protein-coupled receptor internalization / receptor internalization / photoreceptor disc membrane / Inactivation, recovery and regulation of the phototransduction cascade / cell cortex ...G-protein-coupled receptor kinase / rhodopsin kinase activity / G protein-coupled receptor kinase activity / regulation of opsin-mediated signaling pathway / regulation of G protein-coupled receptor signaling pathway / G protein-coupled receptor internalization / receptor internalization / photoreceptor disc membrane / Inactivation, recovery and regulation of the phototransduction cascade / cell cortex / dendrite / neuronal cell body / signal transduction / ATP binding / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.6 Å | ||||||
Authors | Allen, S.J. / Parthasarathy, G. / Soisson, S. / Munshi, S. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2015 Title: Structure and Function of the Hypertension Variant A486V of G Protein-coupled Receptor Kinase 4. Authors: Allen, S.J. / Parthasarathy, G. / Darke, P.L. / Diehl, R.E. / Ford, R.E. / Hall, D.L. / Johnson, S.A. / Reid, J.C. / Rickert, K.W. / Shipman, J.M. / Soisson, S.M. / Zuck, P. / Munshi, S.K. / Lumb, K.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4yhj.cif.gz | 213.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4yhj.ent.gz | 170 KB | Display | PDB format |
PDBx/mmJSON format | 4yhj.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/yh/4yhj ftp://data.pdbj.org/pub/pdb/validation_reports/yh/4yhj | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 67447.016 Da / Num. of mol.: 2 / Mutation: C563S, C578S Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: GRK4, GPRK2L, GPRK4 / Production host: Spodoptera frugiperda (fall armyworm) References: UniProt: P32298, G-protein-coupled receptor kinase #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.61 Å3/Da / Density % sol: 52.85 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop Details: in 0.1 M sodium citrate, pH 5-6.5, 15-27.5 % (w/v) polyethylene glycol 3350 (PEG3350) |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 17-ID / Wavelength: 1 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: May 2, 2009 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→47 Å / Num. obs: 44032 / % possible obs: 99.6 % / Redundancy: 9 % / Net I/σ(I): 10.6 |
-Processing
Software |
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Refinement | Resolution: 2.6→47 Å / Cor.coef. Fo:Fc: 0.9374 / Cor.coef. Fo:Fc free: 0.8925 / SU R Cruickshank DPI: 0.439 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.437 / SU Rfree Blow DPI: 0.274 / SU Rfree Cruickshank DPI: 0.278
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Displacement parameters | Biso max: 152.51 Å2 / Biso mean: 50.2 Å2 / Biso min: 18.69 Å2
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Refine analyze | Luzzati coordinate error obs: 0.341 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.6→47 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.6→2.67 Å / Total num. of bins used: 20
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