温度: 285 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 7 詳細: 1 microliter of VP1 at 10 mg/ml in 25 mM Na-HEPES, pH 7.8, 100 mM NaCl mixed with 2 microliter of crystallization buffer [25 mM Na-MES, pH 6.5, 1.5% (w/v) PEG 3350] and allowing the drop to ...詳細: 1 microliter of VP1 at 10 mg/ml in 25 mM Na-HEPES, pH 7.8, 100 mM NaCl mixed with 2 microliter of crystallization buffer [25 mM Na-MES, pH 6.5, 1.5% (w/v) PEG 3350] and allowing the drop to equilibrate at 12 C by hanging-drop vapor diffusion with a well solution identical in composition to the drop except for the protein. With micro-seeding, thin, plate-like crystals appeared after 1 day and grew to full size in about two weeks, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K
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データ収集
回折
平均測定温度: 100 K
放射光源
由来: シンクロトロン / サイト: ALS / ビームライン: 8.2.1 / 波長: 1.1808 Å
モノクロメーター: Double crystal, Si(111) / プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 1.1808 Å / 相対比: 1
Reflection
冗長度: 3.9 % / Av σ(I) over netI: 15.7 / 数: 108692 / Rmerge(I) obs: 0.055 / Χ2: 1.04 / D res high: 2.9 Å / D res low: 30 Å / Num. obs: 28000 / % possible obs: 99.8
Diffraction reflection shell
最高解像度 (Å)
最低解像度 (Å)
% possible obs (%)
ID
Rmerge(I) obs
Chi squared
Redundancy
6.23
30
99.5
1
0.022
1.03
3.7
4.95
6.23
99.9
1
0.038
1.083
3.9
4.33
4.95
99.9
1
0.031
1.089
4
3.93
4.33
100
1
0.041
1.008
4
3.65
3.93
100
1
0.065
1.018
4
3.44
3.65
99.8
1
0.092
1.06
4
3.27
3.44
99.7
1
0.137
1.062
3.9
3.12
3.27
99.9
1
0.208
0.992
3.8
3
3.12
99.8
1
0.319
1.04
3.8
2.9
3
99.1
1
0.426
1.035
3.7
反射
解像度: 2.9→30 Å / Num. obs: 28000 / % possible obs: 99.8 % / 冗長度: 3.9 % / Rmerge(I) obs: 0.055 / Χ2: 1.042 / Net I/σ(I): 15.7
反射 シェル
解像度 (Å)
冗長度 (%)
Rmerge(I) obs
Num. unique all
Χ2
% possible all
2.9-3
3.7
0.426
2693
1.035
99.1
3-3.12
3.8
0.319
2774
1.04
99.8
3.12-3.27
3.8
0.208
2759
0.992
99.9
3.27-3.44
3.9
0.137
2765
1.062
99.7
3.44-3.65
4
0.092
2790
1.06
99.8
3.65-3.93
4
0.065
2750
1.018
100
3.93-4.33
4
0.041
2820
1.008
100
4.33-4.95
4
0.031
2819
1.089
99.9
4.95-6.23
3.9
0.038
2857
1.083
99.9
6.23-30
3.7
0.022
2973
1.03
99.5
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位相決定
位相決定
手法: 多波長異常分散
Phasing MAD
D res high: 2.9 Å / D res low: 47.33 Å / FOM acentric: 0.284 / FOM centric: 0.202 / Reflection acentric: 24408 / Reflection centric: 2968