PDB-4yfa: Structure of N-acylhomoserine lactone acylase MacQ in complex wit...

ID or keywords:

Entry

Database: PDB / ID: 4yfa

Title

Structure of N-acylhomoserine lactone acylase MacQ in complex with decanoic acid

Components

(Protein related to penicillin acylase) x 3

Keywords

HYDROLASE / Acylase / product complex / Ntn-hydrolase fold

Function / homology

Function and homology information

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides / antibiotic biosynthetic process
Similarity search - Function

Biological species

Acidovorax sp. MR-S7 (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 2.2 Å

Authors

Yasutake, Y. / Kusada, H. / Kimura, N.

Citation

Journal: Sci Rep / Year: 2017
Title: Bifunctional quorum-quenching and antibiotic-acylase MacQ forms a 170-kDa capsule-shaped molecule containing spacer polypeptides
Authors: Yasutake, Y. / Kusada, H. / Ebuchi, T. / Hanada, S. / Kamagata, Y. / Tamura, T. / Kimura, N.

History

Deposition	Feb 25, 2015	Deposition site: RCSB / Processing site: PDBJ
Revision 1.0	Mar 2, 2016	Provider: repository / Type: Initial release
Revision 1.1	Aug 30, 2017	Group: Data collection / Database references / Derived calculations Category: citation / citation_author ...citation / citation_author / diffrn_source / pdbx_struct_oper_list Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _diffrn_source.pdbx_synchrotron_site / _pdbx_struct_oper_list.symmetry_operation
Revision 1.2	Nov 8, 2023	Group: Data collection / Database references / Refinement description Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

Structure viewer	Molecule: MolmilJmol/JSmol

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Validation report

Summary document	4yfa_validation.pdf.gz	535.9 KB	Display	wwPDB validaton report
Full document	4yfa_full_validation.pdf.gz	565.8 KB	Display
Data in XML	4yfa_validation.xml.gz	109.1 KB	Display
Data in CIF	4yfa_validation.cif.gz	153.5 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/yf/4yfa ftp://data.pdbj.org/pub/pdb/validation_reports/yf/4yfa	HTTPS FTP

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Related structure data

Related structure data	4yf9C 4yfbC 5c9iC 2wybS C: citing same article (ref.) S: Starting model for refinement
Similar structure data	4yf9-1 5c9i-1 4yfb-1 5c9i-2 4yf9-2 4yfb-2 6kj0-d 6l7g-d Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#166 - Oct 2013 Proteasome similarity (1) #80 - Aug 2006 AAA+ Proteases similarity (1) #261 - Sep 2021 DNA-Sequencing Nanopores similarity (1) #176 - Aug 2014 Dynein similarity (1) #60 - Dec 2004 Ubiquitin similarity (1) #22 - Oct 2001 Photosystem I similarity (1) #262 - Oct 2021 Fifty Years of Open Access to PDB Structures similarity (1) #29 - May 2002 Penicillin-binding Proteins similarity (1) #95 - Nov 2007 Multidrug Resistance Transporters similarity (1)

PDB pages

PDBj /

wwPDB /

NCBI

Related items in Molecule of the Month

#166 - Oct 2013
Proteasome
similarity (1)
#80 - Aug 2006
AAA+ Proteases
similarity (1)
#261 - Sep 2021
DNA-Sequencing Nanopores
similarity (1)
#176 - Aug 2014
Dynein
similarity (1)
#60 - Dec 2004
Ubiquitin
similarity (1)
#22 - Oct 2001
Photosystem I
similarity (1)
#262 - Oct 2021
Fifty Years of Open Access to PDB Structures
similarity (1)
#29 - May 2002
Penicillin-binding Proteins
similarity (1)
#95 - Nov 2007
Multidrug Resistance Transporters
similarity (1)

Deposited unit

A: Protein related to penicillin acylase

B: Protein related to penicillin acylase

C: Protein related to penicillin acylase

D: Protein related to penicillin acylase

E: Protein related to penicillin acylase

F: Protein related to penicillin acylase

G: Protein related to penicillin acylase

H: Protein related to penicillin acylase

I: Protein related to penicillin acylase

J: Protein related to penicillin acylase

K: Protein related to penicillin acylase

L: Protein related to penicillin acylase

hetero molecules

339 kDa, 12 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: Protein related to penicillin acylase

B: Protein related to penicillin acylase

C: Protein related to penicillin acylase

D: Protein related to penicillin acylase

E: Protein related to penicillin acylase

F: Protein related to penicillin acylase

hetero molecules

defined by author
169 kDa, 6 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

G: Protein related to penicillin acylase

H: Protein related to penicillin acylase

I: Protein related to penicillin acylase

J: Protein related to penicillin acylase

K: Protein related to penicillin acylase

L: Protein related to penicillin acylase

hetero molecules

defined by author
169 kDa, 6 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	85.408, 89.851, 122.710
Angle α, β, γ (deg.)	103.26, 104.84, 106.05
Int Tables number	1
Space group name H-M	P1

#1: Protein	Protein related to penicillin acylase / N-Acyl-L-homoserine lactone acylase / MacQ Mass: 19231.340 Da / Num. of mol.: 4 / Fragment: alpha-chain, UNP residues 29-206 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Acidovorax sp. MR-S7 (bacteria) / Gene: AVS7_00617 / Plasmid: pET19b / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: A0A0A1VBK6
#2: Protein/peptide	Protein related to penicillin acylase / N-Acyl-L-homoserine lactone acylase / MacQ Mass: 2653.900 Da / Num. of mol.: 4 / Fragment: spacer peptide, UNP residues 207-233 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Acidovorax sp. MR-S7 (bacteria) / Gene: AVS7_00617 / Plasmid: pET19b / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: A0A0A1VBK6
#3: Protein	Protein related to penicillin acylase / N-Acyl-L-homoserine lactone acylase / MacQ Mass: 62611.613 Da / Num. of mol.: 4 / Fragment: beta-chain, UNP residues 234-806 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Acidovorax sp. MR-S7 (bacteria) / Gene: AVS7_00617 / Plasmid: pET19b / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: A0A0A1VBK6
#4: Chemical	ChemComp-DKA / DECANOIC ACID Mass: 172.265 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C₁₀H₂₀O₂
#5: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 843 / Source method: isolated from a natural source / Formula: H₂O

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Experiment

Experiment	Method: X-RAY DIFFRACTION

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Sample preparation

Crystal	Density Matthews: 2.45 Å³/Da / Density % sol: 49.78 %
Crystal grow	Temperature: 293 K / Method: vapor diffusion, hanging drop / Details: Tris-HCl, calcium acetate, PEG 3350 / PH range: 7.5-8.5

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Data collection

Diffraction	Mean temperature: 100 K
Diffraction source	Source: SYNCHROTRON / Site: Photon Factory / Beamline: AR-NE3A / Wavelength: 1 Å
Detector	Type: ADSC QUANTUM 270 / Detector: CCD / Date: Jun 6, 2012
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 1 Å / Relative weight: 1
Reflection	Resolution: 2.2→50 Å / Num. obs: 159207 / % possible obs: 98.3 % / Redundancy: 3.9 % / R_merge(I) obs: 0.083 / Net I/σ(I): 23.4
Reflection shell	Resolution: 2.2→2.24 Å / Redundancy: 3.9 % / R_merge(I) obs: 0.735 / Mean I/σ(I) obs: 2.3 / % possible all: 97.3

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Processing

Software

Name	Version	Classification
REFMAC	5.6.0117	refinement
HKL-2000		data reduction
HKL-2000		data scaling
PHASER		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: 2WYB

2wyb

Resolution: 2.2→38.94 Å / Cor.coef. F_o:F_c: 0.961 / Cor.coef. F_o:F_c free: 0.939 / SU B: 14.197 / SU ML: 0.175 / Cross valid method: THROUGHOUT / ESU R: 0.286 / ESU R Free: 0.211 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT

	R_factor	Num. reflection	% reflection	Selection details
R_free	0.23508	8025	5 %	RANDOM
R_work	0.19349	-	-	-
obs	0.19559	151153	98.01 %	-

Solvent computation

Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK

Displacement parameters

B_iso mean: 45.924 Å²

	B_aniso -1	B_aniso -2	B_aniso -3
1-	1.82 Å²	1.27 Å²	-0.67 Å²
2-	-	-0.78 Å²	-0.92 Å²
3-	-	-	-1.1 Å²

Refinement step

Cycle: LAST / Resolution: 2.2→38.94 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	23014	0	46	843	23903

Refine LS restraints

Refine-ID	Type	Dev ideal	Dev ideal target	Number
X-RAY DIFFRACTION	r_bond_refined_d	0.007	0.019	23662
X-RAY DIFFRACTION	r_bond_other_d
X-RAY DIFFRACTION	r_angle_refined_deg	1.179	1.928	32208
X-RAY DIFFRACTION	r_angle_other_deg
X-RAY DIFFRACTION	r_dihedral_angle_1_deg	5.999	5	3017
X-RAY DIFFRACTION	r_dihedral_angle_2_deg	35.539	22.978	1088
X-RAY DIFFRACTION	r_dihedral_angle_3_deg	15.539	15	3397
X-RAY DIFFRACTION	r_dihedral_angle_4_deg	16.686	15	196
X-RAY DIFFRACTION	r_chiral_restr	0.082	0.2	3429
X-RAY DIFFRACTION	r_gen_planes_refined	0.005	0.021	18636
X-RAY DIFFRACTION	r_gen_planes_other
X-RAY DIFFRACTION	r_nbd_refined
X-RAY DIFFRACTION	r_nbd_other
X-RAY DIFFRACTION	r_nbtor_refined
X-RAY DIFFRACTION	r_nbtor_other
X-RAY DIFFRACTION	r_xyhbond_nbd_refined
X-RAY DIFFRACTION	r_xyhbond_nbd_other
X-RAY DIFFRACTION	r_metal_ion_refined
X-RAY DIFFRACTION	r_metal_ion_other
X-RAY DIFFRACTION	r_symmetry_vdw_refined
X-RAY DIFFRACTION	r_symmetry_vdw_other
X-RAY DIFFRACTION	r_symmetry_hbond_refined
X-RAY DIFFRACTION	r_symmetry_hbond_other
X-RAY DIFFRACTION	r_symmetry_metal_ion_refined
X-RAY DIFFRACTION	r_symmetry_metal_ion_other
X-RAY DIFFRACTION	r_mcbond_it
X-RAY DIFFRACTION	r_mcbond_other
X-RAY DIFFRACTION	r_mcangle_it
X-RAY DIFFRACTION	r_mcangle_other
X-RAY DIFFRACTION	r_scbond_it
X-RAY DIFFRACTION	r_scbond_other
X-RAY DIFFRACTION	r_scangle_it
X-RAY DIFFRACTION	r_scangle_other
X-RAY DIFFRACTION	r_long_range_B_refined
X-RAY DIFFRACTION	r_long_range_B_other
X-RAY DIFFRACTION	r_rigid_bond_restr
X-RAY DIFFRACTION	r_sphericity_free
X-RAY DIFFRACTION	r_sphericity_bonded

LS refinement shell

Resolution: 2.202→2.259 Å / Total num. of bins used: 20

	R_factor	Num. reflection	% reflection
R_free	0.334	557	-
R_work	0.289	10704	-
obs	-	-	94.06 %

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	1.6977	-0.3183	0.1104	1.061	-0.3399	1.1317	0.0715	0.2394	0.0527	-0.4235	-0.1265	-0.0212	0.3499	0.5231	0.055	0.4136	0.1443	0.0177	0.2962	0.0336	0.0215	3.1436	6.2454	-15.0561
2	0.9398	-0.2535	-0.0078	1.9367	-0.4509	1.0703	0.0484	0.3203	-0.1459	-0.5287	-0.0395	-0.0497	0.4045	0.405	-0.009	0.5188	0.1969	0.0003	0.3913	-0.0331	0.0428	3.9071	2.6445	-20.1567
3	9.9578	-2.3353	-0.1149	7.6813	0.1421	3.9362	0.5275	0.5467	0.8581	0.1723	-0.455	-0.4	-0.076	0.645	-0.0726	0.3944	0.2452	0.0731	0.6275	0.1259	0.0985	22.6401	0.0138	-3.1364
4	15.8555	3.4061	-0.2772	12.7075	3.5931	5.2031	-0.0674	0.5346	1.3388	0.2187	-0.4752	-0.2134	-0.5485	0.8048	0.5426	0.4981	-0.0018	-0.0055	0.5472	0.1596	0.3896	24.14	1.0354	13.5565
5	23.4238	10.7855	21.4544	5.1586	9.2162	21.9472	0.7921	-0.3249	0.3061	0.5478	-0.4799	0.3426	-0.0521	1.1618	-0.3122	0.6952	-0.1442	0.1787	1.2349	-0.3587	0.3097	7.3155	1.5995	6.0476
6	0.8141	-0.2353	0.1398	1.2663	-0.302	1.3505	0.1212	0.187	-0.0312	-0.2742	-0.1599	-0.1533	0.3471	0.4986	0.0387	0.4057	0.1817	-0.0054	0.2624	-0.0175	0.0556	9.862	-3.8972	-4.6006
7	1.0161	-0.4034	0.4042	1.1567	-0.3644	1.3878	0.1647	0.2192	-0.1607	-0.2196	-0.133	0.0492	0.4693	0.3105	-0.0317	0.5122	0.1504	-0.0601	0.154	-0.0372	0.1313	3.2632	-15.8079	2.6388
8	0.6853	0.0508	0.4003	0.869	-0.1598	2.0652	0.0939	-0.0292	0.0213	-0.0164	-0.0168	0.182	0.0707	0.1017	-0.0771	0.1566	-0.0081	0.0235	0.0229	-0.0087	0.0906	-8.7017	9.0164	12.6977
9	1.434	-0.7903	-0.3671	2.6964	-0.162	0.6455	-0.0158	-0.106	0.0261	0.1079	-0.0318	0.0214	0.3885	0.0445	0.0476	0.3308	-0.0139	0.0523	0.1294	-0.0041	0.037	20.8241	33.3806	22.9309
10	1.6187	-0.185	1.3425	6.4271	2.9371	2.9266	-0.0951	-0.0283	0.012	0.5305	0.3317	-0.4843	0.4194	0.4934	-0.2366	0.2836	0.2181	0.0254	0.4427	-0.0216	0.1285	36.4676	31.3304	25.4846
11	1.1889	-0.1009	-0.1374	1.0018	0.0321	1.1926	-0.1107	-0.0506	0.0016	0.1361	-0.043	-0.119	0.5839	0.0903	0.1537	0.3027	0.0455	0.0527	0.1506	0.0046	0.0735	23.8781	28.1258	23.5315
12	17.781	-6.5245	2.5923	44.7079	4.4816	16.3604	-0.202	0.2705	-1.1223	-0.2189	0.3673	-1.2286	-0.0148	-0.4131	-0.1653	0.9229	0.099	0.1324	0.6087	0.0013	0.2715	35.6838	10.0245	-8.8707
13	0.4531	-0.2718	-1.7479	0.2047	0.9356	7.0591	-0.349	-0.0727	-0.2131	0.2222	0.0319	0.3177	1.1469	0.2319	0.3171	0.6917	0.1724	0.0557	0.4717	-0.0295	1.0719	29.6637	24.3262	-0.6548
14	1.3493	0.6639	-1.0239	1.2383	-0.0057	3.5251	0.0826	0.0132	0.3174	-0.0096	-0.0518	-0.0219	0.0804	0.2624	-0.0308	0.0193	0.0242	0.0479	0.1377	0.0302	0.1728	26.2297	47.2044	11.0468
15	0.8913	-0.2047	-0.1802	0.8363	-0.2275	1.1978	0.0271	0.0194	0.0547	-0.0694	-0.0618	-0.178	0.351	0.3164	0.0347	0.1488	0.0746	0.0671	0.275	0.0202	0.1538	39.2801	33.6257	4.9566
16	0.529	0.0773	0.0664	0.6509	0.4045	1.7663	0.0992	0.1876	0.1633	-0.1748	-0.0472	0.0323	0.1423	-0.2401	-0.0519	0.123	-0.0113	0.0237	0.2606	0.0765	0.136	12.7271	41.0934	-4.6635
17	0.9999	-0.5036	-0.501	1.9392	-0.0639	1.6489	-0.0096	-0.1236	-0.1416	0.2373	-0.0196	0.0626	-0.0021	-0.222	0.0292	0.0717	-0.0679	0.0309	0.1501	-0.0201	0.2001	1.1222	-38.2843	58.8835
18	1.9321	-1.6452	-1.716	7.2868	1.4575	4.8778	-0.1055	0.1618	-0.2936	0.1885	0.0278	0.9054	0.1332	-0.7043	0.0778	0.0576	-0.1108	0.0319	0.3134	-0.0435	0.4897	-14.433	-40.8256	53.3875
19	1.5217	-0.7701	0.4456	2.3172	-0.9096	1.8899	-0.003	-0.1012	-0.1066	0.3472	-0.0566	0.2217	-0.0188	-0.1865	0.0596	0.0837	-0.0741	0.0507	0.1583	-0.0309	0.2257	-2.0665	-35.3719	61.0401
20	13.6187	0.53	-1.8162	24.7789	8.1406	12.0468	-0.3397	-0.62	0.2001	0.8325	0.4643	0.1529	-0.6807	0.5425	-0.1246	0.2778	-0.0216	0.0016	0.4056	0.0508	0.1195	0.1495	-3.0688	45.3116
21	0.1089	-0.9499	-0.7045	8.7533	6.5205	4.8679	0.4196	0.2063	0.0168	-0.0956	-1.1507	0.3724	-0.0764	-1.0681	0.7311	0.5264	0.2137	0.3006	1.0405	0.2286	1.428	4.7082	-21.5357	44.0853
22	1.0681	-0.2711	-0.3282	1.4398	-0.1192	1.1288	-0.0039	0.1631	-0.1114	-0.0774	-0.0175	0.2972	-0.0622	-0.3775	0.0214	0.0329	-0.0095	-0.0386	0.2037	-0.0607	0.2499	-3.4572	-29.9432	38.8006
23	1.7017	0.3209	0.1536	1.4593	0.513	1.6335	0.0781	0.0749	0.1457	-0.2085	0.0267	-0.1181	-0.2329	0.1793	-0.1048	0.0725	-0.0475	0.0729	0.0897	-0.0482	0.2083	28.5531	-18.5842	33.3288
24	1.1409	0.1852	-0.2184	1.0793	0.0765	1.518	-0.0428	-0.1466	-0.2589	0.1221	-0.0504	-0.1627	0.1794	0.0866	0.0932	0.0444	-0.0104	0.0078	0.072	-0.0179	0.2217	17.2691	-42.1043	47.3711
25	2.5219	-0.3628	0.2985	1.9014	-0.1201	1.5274	-0.0443	0.1279	-0.0485	0.0565	0.2413	-0.5269	-0.3135	-0.1083	-0.197	0.1175	-0.0439	-0.0051	0.1562	-0.0313	0.2639	29.5703	2.5502	58.4439
26	1.3963	-0.1911	-1.0313	3.3506	0.5722	2.3242	-0.0192	0.1435	0.1737	-0.1244	0.2455	-0.5864	-0.471	-0.1153	-0.2262	0.1714	-0.0433	0.0102	0.1411	-0.0353	0.3156	31.5193	8.657	56.1089
27	9.1298	-2.4756	5.2649	5.3728	0.1964	6.2095	0.0719	0.5581	-0.2509	0.187	0.2282	0.3136	0.1069	-0.1703	-0.3001	0.0802	0.0577	0.0745	0.3207	0.1163	0.1141	8.442	4.2898	62.639
28	58.7072	-8.0625	2.3525	1.27	-1.0241	3.197	0.5292	-1.5623	-1.6512	-0.0013	0.29	0.4473	-0.2619	0.0727	-0.8192	0.4934	-0.1769	0.1936	0.6701	0.1637	0.5919	-8.219	-6.8862	70.8772
29	2.4745	0.9157	-1.2106	0.3489	-0.4587	0.6087	-0.5173	0.7102	-0.8766	-0.1743	0.2554	-0.255	0.2794	-0.3773	0.262	0.615	0.1718	-0.1639	0.7752	-0.0236	0.9277	9.8531	-6.0676	71.7977
30	1.0713	-0.0185	0.0798	1.7608	0.1206	1.2469	-0.0252	-0.0197	0.0979	0.4799	0.1714	-0.0744	-0.2557	-0.2227	-0.1462	0.2688	0.0416	-0.0287	0.1453	0.033	0.1626	17.3237	7.4809	75.4908
31	0.8178	0.4484	0.2367	3.4163	-0.1392	1.2621	-0.0635	-0.0747	-0.0747	1.0505	0.113	-0.3841	-0.0595	-0.0804	-0.0496	0.4736	0.0777	-0.1671	0.1806	-0.0072	0.16	23.3749	-22.0317	89.2007
32	0.849	0.5166	-0.3626	2.2857	-0.4434	1.3325	-0.1573	-0.0244	-0.0091	0.424	0.2143	-0.4794	0.0895	0.0123	-0.057	0.1886	0.0457	-0.1702	0.1371	-0.0633	0.2469	29.6007	-16.172	76.033

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	A	12 - 95
2	X-RAY DIFFRACTION	2	A	96 - 164
3	X-RAY DIFFRACTION	3	A	165 - 178
4	X-RAY DIFFRACTION	4	B	8 - 17
5	X-RAY DIFFRACTION	5	B	18 - 23
6	X-RAY DIFFRACTION	6	C	1 - 152
7	X-RAY DIFFRACTION	7	C	153 - 297
8	X-RAY DIFFRACTION	8	C	298 - 574
9	X-RAY DIFFRACTION	9	D	11 - 88
10	X-RAY DIFFRACTION	10	D	89 - 115
11	X-RAY DIFFRACTION	11	D	116 - 178
12	X-RAY DIFFRACTION	12	E	8 - 12
13	X-RAY DIFFRACTION	13	E	13 - 22
14	X-RAY DIFFRACTION	14	F	1 - 74
15	X-RAY DIFFRACTION	15	F	75 - 337
16	X-RAY DIFFRACTION	16	F	338 - 574
17	X-RAY DIFFRACTION	17	G	12 - 93
18	X-RAY DIFFRACTION	18	G	94 - 115
19	X-RAY DIFFRACTION	19	G	116 - 178
20	X-RAY DIFFRACTION	20	H	8 - 15
21	X-RAY DIFFRACTION	21	H	16 - 23
22	X-RAY DIFFRACTION	22	I	1 - 337
23	X-RAY DIFFRACTION	23	I	338 - 457
24	X-RAY DIFFRACTION	24	I	458 - 574
25	X-RAY DIFFRACTION	25	J	12 - 95
26	X-RAY DIFFRACTION	26	J	96 - 159
27	X-RAY DIFFRACTION	27	J	160 - 178
28	X-RAY DIFFRACTION	28	K	8 - 12
29	X-RAY DIFFRACTION	29	K	13 - 23
30	X-RAY DIFFRACTION	30	L	1 - 297
31	X-RAY DIFFRACTION	31	L	298 - 389
32	X-RAY DIFFRACTION	32	L	390 - 575

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