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Open data
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Basic information
Entry | Database: PDB / ID: 4ydw | ||||||
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Title | CRYSTAL STRUCTURE OF ANTI-IL4 DARPIN 44C12V5 | ||||||
![]() | DARPIN 44C12V5 | ||||||
![]() | DE NOVO PROTEIN / ALTERNATIVE SCAFFOLD | ||||||
Function / homology | Ankyrin repeat-containing domain / Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat / Alpha Horseshoe / Mainly Alpha![]() | ||||||
Biological species | synthetic construct (others) | ||||||
Method | ![]() ![]() | ||||||
![]() | Teplyakov, A. / Obmolova, G. / Gilliland, G. | ||||||
![]() | ![]() Title: CRYSTAL STRUCTURE OF ANTI-IL4 DARPIN 44C12V5 Authors: Teplyakov, A. / Obmolova, G. / Gilliland, G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 72.1 KB | Display | ![]() |
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PDB format | ![]() | 52.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 426.3 KB | Display | ![]() |
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Full document | ![]() | 427.5 KB | Display | |
Data in XML | ![]() | 14.2 KB | Display | |
Data in CIF | ![]() | 20 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4ydyS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 17887.037 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.08 Å3/Da / Density % sol: 41 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 5.1 Details: 23% PEG 3350, 0.2 M DI-AMMONIUM CITRATE, 3% DIOXANE, 3% ISOPROPANOL |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU SATURN 944 / Detector: CCD / Date: Apr 11, 2014 / Details: VARIMAX HF |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→30 Å / Num. obs: 22846 / % possible obs: 94.2 % / Observed criterion σ(I): -3 / Redundancy: 14.1 % / Biso Wilson estimate: 23.3 Å2 / Rmerge(I) obs: 0.052 / Net I/σ(I): 43.5 |
Reflection shell | Resolution: 1.9→1.95 Å / Redundancy: 8.7 % / Rmerge(I) obs: 0.294 / Mean I/σ(I) obs: 7.8 / % possible all: 71.5 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4YDY Resolution: 1.9→25.744 Å / Cor.coef. Fo:Fc: 0.953 / Cor.coef. Fo:Fc free: 0.93 / SU B: 2.907 / SU ML: 0.087 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.155 / ESU R Free: 0.142
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 19.3 Å2
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Refinement step | Cycle: LAST / Resolution: 1.9→25.744 Å
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Refine LS restraints |
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