Bifunctionallysine-specificdemethylaseandhistidyl-hydroxylaseNO66 / 60S ribosomal protein L8 histidine hydroxylase / Histone lysine demethylase NO66 / Myc-associated ...60S ribosomal protein L8 histidine hydroxylase / Histone lysine demethylase NO66 / Myc-associated protein with JmjC domain / Nucleolar protein 66 / hsNO66 / Ribosomal oxygenase NO66 / ROX
Mass: 51565.191 Da / Num. of mol.: 2 / Fragment: UNP RESIDUES 176-525, 541-641 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: NO66, C14orf169, MAPJD / Plasmid: pET28a / Production host: Escherichia coli (E. coli) References: UniProt: Q9H6W3, Oxidoreductases; Acting on paired donors, with incorporation or reduction of molecular oxygen; With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen ...References: UniProt: Q9H6W3, Oxidoreductases; Acting on paired donors, with incorporation or reduction of molecular oxygen; With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor, [histone H3]-dimethyl-L-lysine36 demethylase
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 0.9791 Å / Relative weight: 1
Reflection
Resolution: 3.3→64.58 Å / Num. obs: 27284 / % possible obs: 98.8 % / Redundancy: 6.2 % / Rmerge(I) obs: 0.101 / Net I/σ(I): 12.4
Reflection shell
Resolution: 3.3→3.48 Å / Redundancy: 5.6 % / Rmerge(I) obs: 0.613 / Mean I/σ(I) obs: 3 / % possible all: 98
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Processing
Software
Name
Version
Classification
REFMAC
5.7.0032
refinement
MOSFLM
dataprocessing
PHASER
phasing
Coot
modelbuilding
Refinement
Resolution: 3.3→50 Å / Cor.coef. Fo:Fc: 0.925 / Cor.coef. Fo:Fc free: 0.884 / SU B: 23.085 / SU ML: 0.373 / Cross valid method: THROUGHOUT / ESU R Free: 0.478 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.27096
1372
5 %
RANDOM
Rwork
0.21708
-
-
-
obs
0.21973
25870
98.48 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK