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- PDB-4xuq: Structure of the CBM22-2 xylan-binding domain from Paenibacillus ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4xuq | |||||||||
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Title | Structure of the CBM22-2 xylan-binding domain from Paenibacillus barcinonensis Xyn10C in complex with xylotriose | |||||||||
![]() | Endo-1,4-beta-xylanase C | |||||||||
![]() | SUGAR BINDING PROTEIN / Binding Site / Carbohydrates / Enzyme Stability / Substrate Specificity / Temperature / Endo-1 / 4-beta-xylanase / Xylan-binding domain / Thermophilic enzymes / Thermostabilizing Domains / Xylotriose | |||||||||
Function / homology | ![]() endo-1,4-beta-xylanase activity / endo-1,4-beta-xylanase / xylan catabolic process / carbohydrate binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Sainz-Polo, M.A. / Sanz-Aparicio, J. | |||||||||
![]() | ![]() Title: Exploring Multimodularity in Plant Cell Wall Deconstruction: STRUCTURAL AND FUNCTIONAL ANALYSIS OF Xyn10C CONTAINING THE CBM22-1-CBM22-2 TANDEM. Authors: Sainz-Polo, M.A. / Gonzalez, B. / Menendez, M. / Pastor, F.I. / Sanz-Aparicio, J. #1: Journal: Acta Crystallogr F Struct Biol Commun / Year: 2015 Title: Crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of Paenibacillus barcinonensis xylanase 10C containing the CBM22-1-CBM22-2 tandem. Authors: Sainz-Polo, M.A. / Gonzalez, B. / Pastor, F.I. / Sanz-Aparicio, J. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 112.1 KB | Display | ![]() |
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PDB format | ![]() | 84.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4w8lC ![]() 4xunSC ![]() 4xuoC ![]() 4xupC ![]() 4xurC ![]() 4xutC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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3 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: _ / Beg auth comp-ID: ALA / Beg label comp-ID: ALA / End auth comp-ID: THR / End label comp-ID: THR / Refine code: _ / Auth seq-ID: 172 - 332 / Label seq-ID: 14 - 174
NCS ensembles :
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Components
#1: Protein | Mass: 19920.992 Da / Num. of mol.: 3 / Fragment: UNP residues 186-366 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Polysaccharide | beta-D-xylopyranose-(1-4)-beta-D-xylopyranose / 4beta-beta-xylobiose | #3: Sugar | ChemComp-XYP / #4: Chemical | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.23 Å3/Da / Density % sol: 44.8 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 5 Details: 1.95M Sodium malonate. Ratio protein/precipitant=1/0.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Sep 26, 2013 |
Radiation | Monochromator: Si(111) channel-cut / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 1.95→48.42 Å / Num. obs: 33856 / % possible obs: 99.9 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Redundancy: 9.3 % / Rmerge(I) obs: 0.09 / Rsym value: 0.031 / Net I/av σ(I): 6.2 / Net I/σ(I): 17.5 / Num. measured all: 315365 |
Reflection shell | Resolution: 1.95→2.05 Å / Redundancy: 9 % / Rmerge(I) obs: 0.289 / Mean I/σ(I) obs: 7.6 / Num. unique all: 4948 / % possible all: 99.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4xun Resolution: 1.95→48.42 Å / Cor.coef. Fo:Fc: 0.95 / Cor.coef. Fo:Fc free: 0.932 / SU B: 3.65 / SU ML: 0.106 / Cross valid method: THROUGHOUT / ESU R: 0.173 / ESU R Free: 0.148 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 21.523 Å2
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Refinement step | Cycle: LAST / Resolution: 1.95→48.42 Å
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Refine LS restraints |
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