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Yorodumi- PDB-4xme: Crystal structure of nitrophorin 7 from Rhodnius prolixus at pH 7... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4xme | ||||||
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Title | Crystal structure of nitrophorin 7 from Rhodnius prolixus at pH 7.8 complexed with NO | ||||||
Components | Nitrophorin-7 | ||||||
Keywords | TRANSPORT PROTEIN / Heme / NO transporter / OXIDOREDUCTASE | ||||||
Function / homology | Function and homology information intracellular nitric oxide homeostasis / vasodilation in another organism / response to histamine / nitrite dismutase / negative regulation of coagulation / histamine binding / nitric oxide binding / platelet aggregation / oxidoreductase activity / extracellular region / metal ion binding Similarity search - Function | ||||||
Biological species | Rhodnius prolixus (insect) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.29 Å | ||||||
Authors | Ogata, H. | ||||||
Citation | Journal: F1000Res / Year: 2015 Title: Structure and dynamics of the membrane attaching nitric oxide transporter nitrophorin 7. Authors: Knipp, M. / Ogata, H. / Soavi, G. / Cerullo, G. / Allegri, A. / Abbruzzetti, S. / Bruno, S. / Viappiani, C. / Bidon-Chanal, A. / Luque, F.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4xme.cif.gz | 97.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4xme.ent.gz | 73 KB | Display | PDB format |
PDBx/mmJSON format | 4xme.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/xm/4xme ftp://data.pdbj.org/pub/pdb/validation_reports/xm/4xme | HTTPS FTP |
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-Related structure data
Related structure data | 4xmcC 4xmdC 4xmfC 4xmgC 4xmhC 3mvfS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 20759.660 Da / Num. of mol.: 1 / Fragment: UNP RESIDUES 22-205 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rhodnius prolixus (insect) / Production host: Escherichia coli (E. coli) / References: UniProt: Q6PQK2, nitrite dismutase |
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#2: Chemical | ChemComp-HEM / |
#3: Chemical | ChemComp-NO / |
#4: Chemical | ChemComp-GOL / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.18 Å3/Da / Density % sol: 43.58 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop Details: 25%(w/v) PEG 3350, 0.1 M bis-Tris propane pH 7.8, 0.25%(w/v) Gly-Gly, 0.25%(w/v) Gly-Gly-Gly, 0.25%(w/v) Gly- Gly-Gly-Gly, 0.25%(w/v) pentaglycine, 0.02 M HEPES sodium pH 6.8. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SLS / Beamline: X06SA / Wavelength: 1 Å |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Dec 20, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.29→34.71 Å / Num. obs: 83829 / % possible obs: 94 % / Redundancy: 1.7 % / Rmerge(I) obs: 0.032 / Net I/σ(I): 9.5 |
Reflection shell | Resolution: 1.29→1.32 Å / Redundancy: 1.5 % / Rmerge(I) obs: 0.925 / Mean I/σ(I) obs: 1 / % possible all: 84 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3MVF Resolution: 1.29→34.71 Å / SU ML: 0.3 / Cross valid method: FREE R-VALUE / σ(F): 1.99 / Phase error: 22.49 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.73 Å / VDW probe radii: 1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 56.621 Å2 / ksol: 0.404 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 1.29→34.71 Å
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Refine LS restraints |
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LS refinement shell |
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