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Open data
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Basic information
Entry | Database: PDB / ID: 4v2c | ||||||
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Title | mouse FLRT2 LRR domain in complex with rat Unc5D Ig1 domain | ||||||
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![]() | APOPTOSIS / UNC5 / UNCOORDINATED-5 / LEUCINE-RICH REPEAT | ||||||
Function / homology | ![]() cell adhesion involved in heart morphogenesis / Downstream signaling of activated FGFR1 / netrin receptor activity / basement membrane organization / regulation of neuron migration / cell-cell adhesion via plasma-membrane adhesion molecules / fibroblast growth factor receptor binding / chemorepellent activity / pyramidal neuron differentiation / positive regulation of synapse assembly ...cell adhesion involved in heart morphogenesis / Downstream signaling of activated FGFR1 / netrin receptor activity / basement membrane organization / regulation of neuron migration / cell-cell adhesion via plasma-membrane adhesion molecules / fibroblast growth factor receptor binding / chemorepellent activity / pyramidal neuron differentiation / positive regulation of synapse assembly / fibroblast growth factor receptor signaling pathway / heart morphogenesis / axon guidance / cell-cell junction / presynaptic membrane / postsynaptic membrane / neuron projection / focal adhesion / glutamatergic synapse / endoplasmic reticulum membrane / apoptotic process / cell surface / extracellular space / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Seiradake, E. / del Toro, D. / Nagel, D. / Cop, F. / Haertl, R. / Ruff, T. / Seyit-Bremer, G. / Harlos, K. / Border, E.C. / Acker-Palmer, A. ...Seiradake, E. / del Toro, D. / Nagel, D. / Cop, F. / Haertl, R. / Ruff, T. / Seyit-Bremer, G. / Harlos, K. / Border, E.C. / Acker-Palmer, A. / Jones, E.Y. / Klein, R. | ||||||
![]() | ![]() Title: Flrt Structure: Balancing Repulsion and Cell Adhesion in Cortical and Vascular Development Authors: Seiradake, E. / Del Toro, D. / Nagel, D. / Cop, F. / Haertl, R. / Ruff, T. / Seyit-Bremer, G. / Harlos, K. / Border, E.C. / Acker-Palmer, A. / Jones, E.Y. / Klein, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 360.6 KB | Display | ![]() |
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PDB format | ![]() | 300.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4v2aC ![]() 4v2bSC ![]() 4v2dC ![]() 4v2eC C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 37037.426 Da / Num. of mol.: 2 / Fragment: LRR DOMAIN, RESIDUES 35-362 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Protein | Mass: 18128.379 Da / Num. of mol.: 2 / Fragment: IG1 DOMAIN, RESIDUES 1-161 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Sequence details | N-TERMINUS IS CLEAVED DURING EXPRESSION. CONTAINS C- TERMINAL HIS TAG. N-TERMINUS IS CLEAVED. C- ...N-TERMINUS IS CLEAVED DURING EXPRESSION | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.04 Å3/Da / Density % sol: 69.54 % / Description: NONE |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.92 Å / Relative weight: 1 |
Reflection | Resolution: 4→87 Å / Num. obs: 102048 / % possible obs: 98.8 % / Redundancy: 6.9 % / Biso Wilson estimate: 136.77 Å2 / Rmerge(I) obs: 0.17 / Net I/σ(I): 8.4 |
Reflection shell | Resolution: 4→4.1 Å / Redundancy: 5.9 % / Mean I/σ(I) obs: 0.87 / % possible all: 87.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 4V2B Resolution: 4→87.84 Å / Cor.coef. Fo:Fc: 0.6709 / Cor.coef. Fo:Fc free: 0.6392 / Cross valid method: THROUGHOUT / σ(F): 0 / SU Rfree Blow DPI: 0.877 Details: IDEAL-DIST CONTACT TERM CONTACT SETUP. ALL ATOMS HAVE CCP4 ATOM TYPE FROM LIBRARY
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Displacement parameters | Biso mean: 111.61 Å2
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Refine analyze | Luzzati coordinate error obs: 1.593 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 4→87.84 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 4→4.32 Å / Total num. of bins used: 7
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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