+Open data
-Basic information
Entry | Database: PDB / ID: 4uxv | ||||||
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Title | Cytoplasmic domain of bacterial cell division protein EzrA | ||||||
Components | SEPTATION RING FORMATION REGULATOR EZRA | ||||||
Keywords | CELL CYCLE / DIVISOME / FTSZ / CELL WALL / TUBULIN / SPECTRIN / Z-RING | ||||||
Function / homology | Septation ring formation regulator EzrA / Septation ring formation regulator, EzrA / septin ring assembly / septin ring / division septum assembly / positive regulation of cell division / membrane => GO:0016020 / plasma membrane / Septation ring formation regulator EzrA Function and homology information | ||||||
Biological species | BACILLUS SUBTILIS SUBSP. SUBTILIS STR. 168 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 3.961 Å | ||||||
Authors | Cleverley, R.M. / Barrett, J.R. / Basle, A. / Khai-Bui, N. / Hewitt, L. / Solovyova, A. / Xu, Z. / Daniela, R.A. / Dixon, N.E. / Harry, E.J. ...Cleverley, R.M. / Barrett, J.R. / Basle, A. / Khai-Bui, N. / Hewitt, L. / Solovyova, A. / Xu, Z. / Daniela, R.A. / Dixon, N.E. / Harry, E.J. / Oakley, A.J. / Vollmer, W. / Lewis, R.J. | ||||||
Citation | Journal: Nat.Commun. / Year: 2014 Title: Structure and Function of a Spectrin-Like Regulator of Bacterial Cytokinesis. Authors: Cleverley, R.M. / Barrett, J.R. / Basle, A. / Bui, N.K. / Hewitt, L. / Solovyova, A. / Xu, Z. / Daniel, R.A. / Dixon, N.E. / Harry, E.J. / Oakley, A.J. / Vollmer, W. / Lewis, R.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4uxv.cif.gz | 118.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4uxv.ent.gz | 92.8 KB | Display | PDB format |
PDBx/mmJSON format | 4uxv.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 4uxv_validation.pdf.gz | 426.2 KB | Display | wwPDB validaton report |
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Full document | 4uxv_full_validation.pdf.gz | 435.4 KB | Display | |
Data in XML | 4uxv_validation.xml.gz | 20 KB | Display | |
Data in CIF | 4uxv_validation.cif.gz | 26.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ux/4uxv ftp://data.pdbj.org/pub/pdb/validation_reports/ux/4uxv | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 63171.113 Da / Num. of mol.: 1 / Fragment: CYTOPLASMIC DOMAIN, RESIDUES 22-562 Source method: isolated from a genetically manipulated source Source: (gene. exp.) BACILLUS SUBTILIS SUBSP. SUBTILIS STR. 168 (bacteria) Production host: ESCHERICHIA COLI B (bacteria) / Strain (production host): B834(DE3) / References: UniProt: O34894 |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 6.91 Å3/Da / Density % sol: 82.2 % / Description: NONE |
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Crystal grow | pH: 6.7 Details: 50 MM MES PH 6.7, 1 M SODIUM CITRATE, 0.2M NDSB-201, 0.1M AMMONIUM SULPHATE |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I04-1 / Wavelength: 0.9173 |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Oct 16, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9173 Å / Relative weight: 1 |
Reflection | Resolution: 3.95→29.17 Å / Num. obs: 15330 / % possible obs: 99.6 % / Observed criterion σ(I): 0 / Redundancy: 22.4 % / Biso Wilson estimate: 130.78 Å2 / Rmerge(I) obs: 0.1 / Net I/σ(I): 20.8 |
Reflection shell | Resolution: 3.95→4.16 Å / Redundancy: 4.9 % / Rmerge(I) obs: 0.35 / Mean I/σ(I) obs: 3.5 / % possible all: 99.2 |
-Processing
Software |
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Refinement | Method to determine structure: SAD Starting model: NONE Resolution: 3.961→29.044 Å / SU ML: 0.64 / σ(F): 1.63 / Phase error: 43.53 / Stereochemistry target values: MLHL
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 155.6 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.961→29.044 Å
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Refine LS restraints |
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LS refinement shell |
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