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Open data
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Basic information
Entry | Database: PDB / ID: 4ux3 | ||||||
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Title | cohesin Smc3-HD:Scc1-N complex from yeast | ||||||
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![]() | PROTEIN BINDING / COHESIN / MITOSIS / CHROMOSOME SEGREGATION / KLEISIN / SMC | ||||||
Function / homology | ![]() Establishment of Sister Chromatid Cohesion / Resolution of Sister Chromatid Cohesion / meiotic cohesin complex / cohesin loader activity / establishment of meiotic sister chromatid cohesion / mitotic cohesin complex / cohesin complex / SUMOylation of DNA damage response and repair proteins / synaptonemal complex assembly / meiotic sister chromatid cohesion ...Establishment of Sister Chromatid Cohesion / Resolution of Sister Chromatid Cohesion / meiotic cohesin complex / cohesin loader activity / establishment of meiotic sister chromatid cohesion / mitotic cohesin complex / cohesin complex / SUMOylation of DNA damage response and repair proteins / synaptonemal complex assembly / meiotic sister chromatid cohesion / replication-born double-strand break repair via sister chromatid exchange / establishment of mitotic sister chromatid cohesion / mitotic chromosome condensation / reciprocal meiotic recombination / sister chromatid cohesion / mitotic sister chromatid cohesion / protein acetylation / chromosome, centromeric region / condensed nuclear chromosome / double-strand break repair / double-stranded DNA binding / cell division / apoptotic process / DNA damage response / chromatin binding / protein kinase binding / ATP hydrolysis activity / mitochondrion / ATP binding / identical protein binding / nucleus Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Gligoris, T.G. / Nasmyth, K. / Lowe, J. | ||||||
![]() | ![]() Title: Closing the Cohesin Ring: Structure and Function of its Smc3-Kleisin Interface. Authors: Gligoris, T.G. / Scheinost, J.C. / Burmann, F. / Petela, N. / Chan, K. / Uluocak, P. / Beckouet, F. / Gruber, S. / Nasmyth, K. / Lowe, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 228.8 KB | Display | ![]() |
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PDB format | ![]() | 185 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 710.4 KB | Display | ![]() |
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Full document | ![]() | 765.3 KB | Display | |
Data in XML | ![]() | 28.9 KB | Display | |
Data in CIF | ![]() | 38 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1xexS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 62072.840 Da / Num. of mol.: 1 Fragment: HEAD DOMAIN WITH COILED COIL SEGMENT, RESIDUES 2-261 AND RESIDUES 970-1230 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() |
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#2: Protein | Mass: 13488.373 Da / Num. of mol.: 1 / Fragment: N-TERMINAL DOMAIN, RESIDUES 1-155 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() |
#3: Chemical | ChemComp-MG / |
#4: Chemical | ChemComp-AGS / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.27 Å3/Da / Density % sol: 60 % / Description: NONE |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97957 Å / Relative weight: 1 |
Reflection | Resolution: 3.3→30 Å / Num. obs: 15350 / % possible obs: 100 % / Observed criterion σ(I): 0 / Redundancy: 25.6 % / Rmerge(I) obs: 0.11 / Net I/σ(I): 17.7 |
Reflection shell | Resolution: 3.3→3.48 Å / Redundancy: 26.6 % / Rmerge(I) obs: 1.5 / Mean I/σ(I) obs: 2.2 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1XEX Resolution: 3.3→30 Å / Cor.coef. Fo:Fc: 0.946 / Cor.coef. Fo:Fc free: 0.924 / SU B: 106.2 / SU ML: 0.781 / Cross valid method: THROUGHOUT / ESU R Free: 0.585 Stereochemistry target values: MAXIMUM LIKELIHOOD WITH PHASES Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 182.117 Å2
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Refinement step | Cycle: LAST / Resolution: 3.3→30 Å
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Refine LS restraints |
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