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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 4ui1 | ||||||
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タイトル | Crystal structure of the human RGMC-BMP2 complex | ||||||
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![]() | SIGNALING PROTEIN / BONE MORPHOGENETIC PROTEIN PATHWAY / HEMOJUVELIN / MORPHOGEN / AXON GUIDANCE / CELL SURFACE RECEPTOR SIGNALING | ||||||
機能・相同性 | ![]() cardiac atrium formation / cardiocyte differentiation / negative regulation of calcium-independent cell-cell adhesion / cardiac jelly development / negative regulation of aldosterone biosynthetic process / embryonic heart tube anterior/posterior pattern specification / atrioventricular canal morphogenesis / negative regulation of cortisol biosynthetic process / mesenchymal cell proliferation involved in ureteric bud development / negative regulation of steroid biosynthetic process ...cardiac atrium formation / cardiocyte differentiation / negative regulation of calcium-independent cell-cell adhesion / cardiac jelly development / negative regulation of aldosterone biosynthetic process / embryonic heart tube anterior/posterior pattern specification / atrioventricular canal morphogenesis / negative regulation of cortisol biosynthetic process / mesenchymal cell proliferation involved in ureteric bud development / negative regulation of steroid biosynthetic process / ameloblast differentiation / positive regulation of extracellular matrix constituent secretion / negative regulation of cardiac muscle cell differentiation / regulation of odontogenesis of dentin-containing tooth / endodermal-mesodermal cell signaling / corticotropin hormone secreting cell differentiation / negative regulation of insulin-like growth factor receptor signaling pathway / thyroid-stimulating hormone-secreting cell differentiation / mesenchyme development / aortic valve development / telencephalon regionalization / positive regulation of odontogenesis / BMP binding / positive regulation of phosphatase activity / positive regulation of cartilage development / proteoglycan metabolic process / heart induction / positive regulation of peroxisome proliferator activated receptor signaling pathway / Netrin-1 signaling / pericardium development / lung vasculature development / BMP receptor complex / co-receptor binding / transferrin receptor binding / telencephalon development / cardiac epithelial to mesenchymal transition / mesenchymal cell differentiation / BMP receptor binding / positive regulation of odontoblast differentiation / endocardial cushion formation / positive regulation of bone mineralization involved in bone maturation / phosphatase activator activity / Transcriptional regulation by RUNX2 / positive regulation of astrocyte differentiation / Signaling by BMP / cellular response to BMP stimulus / cardiac muscle cell differentiation / activin receptor signaling pathway / cardiac muscle tissue morphogenesis / plasma membrane protein complex / positive regulation of ossification / astrocyte differentiation / Molecules associated with elastic fibres / atrioventricular valve morphogenesis / positive regulation of p38MAPK cascade / endocardial cushion morphogenesis / branching involved in ureteric bud morphogenesis / negative regulation of fat cell differentiation / bone mineralization / positive regulation of osteoblast proliferation / odontogenesis of dentin-containing tooth / positive regulation of SMAD protein signal transduction / inner ear development / protein autoprocessing / cellular response to organic cyclic compound / negative regulation of cell cycle / negative regulation of BMP signaling pathway / positive regulation of Wnt signaling pathway / epithelial to mesenchymal transition / positive regulation of fat cell differentiation / cell fate commitment / chondrocyte differentiation / BMP signaling pathway / positive regulation of bone mineralization / positive regulation of osteoblast differentiation / coreceptor activity / side of membrane / positive regulation of epithelial to mesenchymal transition / Notch signaling pathway / positive regulation of neuron differentiation / protein serine/threonine kinase activator activity / osteoclast differentiation / negative regulation of MAP kinase activity / skeletal system development / cytokine activity / animal organ morphogenesis / negative regulation of smooth muscle cell proliferation / response to bacterium / negative regulation of transforming growth factor beta receptor signaling pathway / growth factor activity / protein destabilization / bone development / negative regulation of canonical Wnt signaling pathway / HFE-transferrin receptor complex / multicellular organismal-level iron ion homeostasis / positive regulation of DNA-binding transcription factor activity / positive regulation of miRNA transcription / osteoblast differentiation / Regulation of RUNX2 expression and activity / positive regulation of protein binding 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() ![]() ![]() | ||||||
![]() | Healey, E.G. / Bishop, B. / Elegheert, J. / Bell, C.H. / Padilla-Parra, S. / Siebold, C. | ||||||
![]() | ![]() タイトル: Repulsive Guidance Molecule is a Structural Bridge between Neogenin and Bone Morphogenetic Protein. 著者: Healey, E.G. / Bishop, B. / Elegheert, J. / Bell, C.H. / Padilla-Parra, S. / Siebold, C. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 151.9 KB | 表示 | ![]() |
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PDB形式 | ![]() | 125.9 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 480.8 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 482.4 KB | 表示 | |
XML形式データ | ![]() | 15.5 KB | 表示 | |
CIF形式データ | ![]() | 21.3 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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非結晶学的対称性 (NCS) | NCS oper:
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要素
-タンパク質 , 2種, 4分子 ABCD
#1: タンパク質 | 分子量: 12923.854 Da / 分子数: 2 断片: C-TERMINAL DOMAIN SIGNALING DOMAIN, RESIDUES 283-396 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() #2: タンパク質 | 分子量: 12432.020 Da / 分子数: 2 / 断片: N-TERMINAL DOMAIN, RESIDUES 35-145 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() |
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-非ポリマー , 4種, 96分子 ![](data/chem/img/NO3.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/HOH.gif)
#3: 化合物 | ChemComp-NO3 / #4: 化合物 | #5: 化合物 | #6: 水 | ChemComp-HOH / | |
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-詳細
配列の詳細 | N-TERMINAL DOMAIN OF HUMAN RGMC WITH 3 ADDITIONAL N- TERMINAL RESIDUES (ETG) AND 9 ADDITIONAL C- ...N-TERMINAL DOMAIN OF HUMAN RGMC WITH 3 ADDITIONAL |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.13 Å3/Da / 溶媒含有率: 42 % / 解説: NONE |
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結晶化 | pH: 7.5 / 詳細: 20% (W/V) PEG3350, 0.2 M AMMONIUM NITRATE, PH 7.5 |
-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: ![]() ![]() ![]() |
検出器 | タイプ: DECTRIS PILATUS 6M / 検出器: PIXEL |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.97625 Å / 相対比: 1 |
反射 | 解像度: 2.35→50 Å / Num. obs: 18558 / % possible obs: 99.9 % / Observed criterion σ(I): 2 / 冗長度: 5.7 % / Biso Wilson estimate: 40.32 Å2 / Rmerge(I) obs: 0.14 / Net I/σ(I): 11.7 |
反射 シェル | 解像度: 2.35→2.45 Å / 冗長度: 5.1 % / Rmerge(I) obs: 0.79 / Mean I/σ(I) obs: 2 / % possible all: 100 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]()
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原子変位パラメータ | Biso mean: 54.76 Å2
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Refine analyze | Luzzati coordinate error obs: 0.318 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: LAST / 解像度: 2.35→43.38 Å
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拘束条件 |
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LS精密化 シェル | 解像度: 2.35→2.49 Å / Total num. of bins used: 9
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精密化 TLS | 手法: refined / Refine-ID: X-RAY DIFFRACTION
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精密化 TLSグループ |
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