E3ubiquitin-proteinligaseUHRF2 / Np95/ICBP90-like RING finger protein / Np95-like RING finger protein / Nuclear protein 97 / Nuclear ...Np95/ICBP90-like RING finger protein / Np95-like RING finger protein / Nuclear protein 97 / Nuclear zinc finger protein Np97 / RING finger protein 107 / Ubiquitin-like PHD and RING finger domain-containing protein 2 / Ubiquitin-like-containing PHD and RING finger domains protein 2
分子量: 31359.346 Da / 分子数: 1 / 断片: Tandem Tudor and PHD domains, UNP residues 109-395 / 変異: deletion from 169 to 176, E383A / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: UHRF2, NIRF, RNF107 / プラスミド: pET28-MHL / 発現宿主: Escherichia coli (大腸菌) / 株 (発現宿主): BL21(DE3)-V2R-pRARE2 参照: UniProt: Q96PU4, 合成酵素; C-N結合を形成; 酸-D-アミノ酸リガーゼ(ペプチド合成)
解像度: 2.29→50 Å / Cor.coef. Fo:Fc: 0.943 / Cor.coef. Fo:Fc free: 0.927 / SU B: 13.81 / SU ML: 0.177 / 交差検証法: THROUGHOUT / ESU R: 0.287 / ESU R Free: 0.222 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.25982
721
5.1 %
RANDOM
Rwork
0.22902
-
-
-
obs
0.23067
13552
98.53 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK