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Yorodumi- PDB-4sgb: STRUCTURE OF THE COMPLEX OF STREPTOMYCES GRISEUS PROTEINASE B AND... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4sgb | ||||||
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Title | STRUCTURE OF THE COMPLEX OF STREPTOMYCES GRISEUS PROTEINASE B AND POLYPEPTIDE CHYMOTRYPSIN INHIBITOR-1 FROM RUSSET BURBANK POTATO TUBERS AT 2.1 ANGSTROMS RESOLUTION | ||||||
Components |
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Keywords | COMPLEX(SERINE PROTEINASE-INHIBITOR) | ||||||
Function / homology | Function and homology information streptogrisin B / serine-type endopeptidase inhibitor activity / serine-type endopeptidase activity / proteolysis / extracellular region Similarity search - Function | ||||||
Biological species | Streptomyces griseus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.1 Å | ||||||
Authors | James, M. / Greenblatt, H. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1989 Title: Structure of the complex of Streptomyces griseus proteinase B and polypeptide chymotrypsin inhibitor-1 from Russet Burbank potato tubers at 2.1 A resolution. Authors: Greenblatt, H.M. / Ryan, C.A. / James, M.N. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4sgb.cif.gz | 60.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4sgb.ent.gz | 42.7 KB | Display | PDB format |
PDBx/mmJSON format | 4sgb.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 4sgb_validation.pdf.gz | 391.2 KB | Display | wwPDB validaton report |
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Full document | 4sgb_full_validation.pdf.gz | 396 KB | Display | |
Data in XML | 4sgb_validation.xml.gz | 6.9 KB | Display | |
Data in CIF | 4sgb_validation.cif.gz | 10.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sg/4sgb ftp://data.pdbj.org/pub/pdb/validation_reports/sg/4sgb | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: RESIDUES PRO E 95 IS A CIS PROLINE. |
-Components
#1: Protein | Mass: 18665.285 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptomyces griseus (bacteria) / References: UniProt: P00777 | ||||||
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#2: Protein | Mass: 5509.348 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source References: UniProt: P01080 | ||||||
#3: Chemical | #4: Chemical | ChemComp-CA / | #5: Water | ChemComp-HOH / | Nonpolymer details | TWO SULFATE IONS HAVE BEEN IDENTIFIED. THERE ARE 180 SOLVENT POSITIONS. AMONG THESE, THE ONE AT ...TWO SULFATE IONS HAVE BEEN IDENTIFIED | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.27 Å3/Da / Density % sol: 45.93 % | ||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 292-296 K / Method: vapor diffusion, hanging drop / PH range low: 7.2 / PH range high: 6.8 | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | *PLUS Num. measured all: 12941 |
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-Processing
Software | Name: PROLSQ / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Resolution: 2.1→8 Å /
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Refinement step | Cycle: LAST / Resolution: 2.1→8 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 2.1 Å / Lowest resolution: 8 Å / Num. reflection obs: 12685 / Rfactor obs: 0.142 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |