+Open data
-Basic information
Entry | Database: PDB / ID: 4rzi | ||||||
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Title | Crystal structure of PhaB from Synechocystis sp. PCC 6803 | ||||||
Components | 3-ketoacyl-acyl carrier protein reductase | ||||||
Keywords | OXIDOREDUCTASE / short-chain dehydrogenase/reductase (SDR) family / AcAcCoA reductase | ||||||
Function / homology | Function and homology information acetoacetyl-CoA reductase / acetoacetyl-CoA reductase activity / poly-hydroxybutyrate biosynthetic process / : / fatty acid elongation / oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Similarity search - Function | ||||||
Biological species | Synechocystis sp. PCC 6803 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.891 Å | ||||||
Authors | Xue, S. / Liu, Y. | ||||||
Citation | Journal: Febs Lett. / Year: 2015 Title: Structure-directed construction of a high-performance version of the enzyme FabG from the photosynthetic microorganism Synechocystis sp. PCC 6803. Authors: Liu, Y. / Feng, Y. / Cao, X. / Li, X. / Xue, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4rzi.cif.gz | 136.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4rzi.ent.gz | 109.8 KB | Display | PDB format |
PDBx/mmJSON format | 4rzi.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rz/4rzi ftp://data.pdbj.org/pub/pdb/validation_reports/rz/4rzi | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 25360.074 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Synechocystis sp. PCC 6803 (bacteria) / Gene: fabG, MYO_113190 / Production host: Escherichia coli (E. coli) / References: UniProt: M1M987, UniProt: P73826*PLUS |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.86 Å3/Da / Density % sol: 57 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 0.22M MgAC, 0.1M sodium cacodylater trihydrate pH6.5, 18%PEG8000 , VAPOR DIFFUSION, HANGING DROP, temperature 289K |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL17U / Wavelength: 1.0001 Å | ||||||||||||||||||
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Jun 6, 2014 | ||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||
Radiation wavelength | Wavelength: 1.0001 Å / Relative weight: 1 | ||||||||||||||||||
Reflection | Resolution: 2.89→50 Å / Num. obs: 20301 / % possible obs: 97 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 42 % | ||||||||||||||||||
Reflection shell |
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.891→38.765 Å / SU ML: 0.36 / σ(F): 1.34 / Phase error: 24.76 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.891→38.765 Å
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Refine LS restraints |
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LS refinement shell |
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