Kuenenia stuttgartiensis hydroxylamine oxidoreductase cryoprotected with ethylene glycol
Components
Similar to hydroxylamine oxidoreductase hao
Keywords
OXIDOREDUCTASE / P468 cofactor
Function / homology
Function and homology information
hydroxylamine oxidase / anaerobic respiration, using ammonium as electron donor / hydroxylamine oxidoreductase activity / anammoxosome / protein homotrimerization / nitric oxide biosynthetic process / heme binding / identical protein binding / metal ion binding Similarity search - Function
Mass: 18.015 Da / Num. of mol.: 509 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 3.26 Å3/Da / Density % sol: 62.27 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.4 Details: 1.3 M ammonium sulfate, 0.05- 0.1 M sodium phosphate buffer, pH 7.4 and the detergent additive cyclohexylbutanoyl-N-hydroxyethylglucamide (C-HEGA-10) at a final concentration of 35 mM, VAPOR ...Details: 1.3 M ammonium sulfate, 0.05- 0.1 M sodium phosphate buffer, pH 7.4 and the detergent additive cyclohexylbutanoyl-N-hydroxyethylglucamide (C-HEGA-10) at a final concentration of 35 mM, VAPOR DIFFUSION, HANGING DROP, temperature 293K