温度: 298 K / 手法: monoolein lipidic cubic phase / pH: 6.5 詳細: Peptide combined with monoolein at 15-20% (w/w) by codissolving in trifluoroethanol, LCP generated by mixing vacuum-dried solid components with water at 60:40 lipid:water ratio. 200 nL LCP ...詳細: Peptide combined with monoolein at 15-20% (w/w) by codissolving in trifluoroethanol, LCP generated by mixing vacuum-dried solid components with water at 60:40 lipid:water ratio. 200 nL LCP bolus combined with 1 uL solution containing 0.1 M ADA pH 6.5, 24% MPD in glass sandwich plates. Crystals grew in less than 1 day. , monoolein lipidic cubic phase, temperature 298K
解像度: 2→19.39 Å / Cor.coef. Fo:Fc: 0.927 / Cor.coef. Fo:Fc free: 0.93 / SU B: 3.934 / SU ML: 0.11 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 0.194 / ESU R Free: 0.169 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT U VALUES : REFINED INDIVIDUALLY
Rfactor
反射数
%反射
Selection details
Rfree
0.2956
283
4.8 %
RANDOM
Rwork
0.2808
-
-
-
obs
0.2816
5919
96.64 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK