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Yorodumi- PDB-2kio: NMR structure of the oxidized yeast TOR1 FATC domain bound to DPC... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2kio | ||||||
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Title | NMR structure of the oxidized yeast TOR1 FATC domain bound to DPC micelles at 318K | ||||||
Components | Serine/threonine-protein kinase TOR1 | ||||||
Keywords | TRANSFERASE / protein / dpc micelle / membrane-mimetic / ATP-binding / Cell cycle / Cell membrane / Kinase / Membrane / Nucleotide-binding / Serine/threonine-protein kinase / Vacuole | ||||||
Function / homology | Function and homology information regulation of snRNA pseudouridine synthesis / regulation of sphingolipid biosynthetic process / mitochondria-nucleus signaling pathway / fungal-type cell wall organization / TORC2 complex / TORC1 complex / TORC1 signaling / fungal-type vacuole membrane / cellular response to nitrogen starvation / negative regulation of macroautophagy ...regulation of snRNA pseudouridine synthesis / regulation of sphingolipid biosynthetic process / mitochondria-nucleus signaling pathway / fungal-type cell wall organization / TORC2 complex / TORC1 complex / TORC1 signaling / fungal-type vacuole membrane / cellular response to nitrogen starvation / negative regulation of macroautophagy / nucleolar large rRNA transcription by RNA polymerase I / TOR signaling / response to endoplasmic reticulum stress / negative regulation of autophagy / response to nutrient / meiotic cell cycle / regulation of cell growth / translational initiation / ribosome biogenesis / cellular response to heat / cellular response to oxidative stress / non-specific serine/threonine protein kinase / regulation of cell cycle / protein kinase activity / endosome membrane / protein phosphorylation / Golgi membrane / protein serine kinase activity / protein serine/threonine kinase activity / DNA damage response / protein-containing complex binding / ATP binding / nucleus / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | SOLUTION NMR / torsion angle dynamics, simulated annealing | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Dames, S.A. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2010 Title: Structural Basis for the Association of the Redox-sensitive Target of Rapamycin FATC Domain with Membrane-mimetic Micelles. Authors: Dames, S.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2kio.cif.gz | 214.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2kio.ent.gz | 183.2 KB | Display | PDB format |
PDBx/mmJSON format | 2kio.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2kio_validation.pdf.gz | 340.7 KB | Display | wwPDB validaton report |
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Full document | 2kio_full_validation.pdf.gz | 458.2 KB | Display | |
Data in XML | 2kio_validation.xml.gz | 16.7 KB | Display | |
Data in CIF | 2kio_validation.cif.gz | 25.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ki/2kio ftp://data.pdbj.org/pub/pdb/validation_reports/ki/2kio | HTTPS FTP |
-Related structure data
Related structure data | 2kitC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 3963.473 Da / Num. of mol.: 1 / Fragment: yeast TOR1 FATC domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: DRR1, J1803, TOR1, YJR066W / Plasmid: pGEV2 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / Variant (production host): Rosetta (DE3) References: UniProt: P35169, non-specific serine/threonine protein kinase |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 150 / pH: 6.5 / Pressure: ambient / Temperature: 318 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics, simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||||||||||||||||||
NMR constraints | NOE constraints total: 932 / Disulfide bond constraints total count: 1 / Protein chi angle constraints total count: 15 / Protein phi angle constraints total count: 25 / Protein psi angle constraints total count: 21 | ||||||||||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |