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- PDB-4rpt: The 1.35A structure of a viral RNase L antagonist reveals basis f... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4rpt | ||||||
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Title | The 1.35A structure of a viral RNase L antagonist reveals basis for the 2'-5'-oligoadenylate binding and enzyme activity. | ||||||
![]() | Capping enzyme protein | ||||||
![]() | VIRAL PROTEIN / 2H phosphodiesterase | ||||||
Function / homology | ![]() : / Hydrolases; Acting on ester bonds; Phosphoric-diester hydrolases / phosphoric diester hydrolase activity / mRNA guanylyltransferase activity / mRNA guanylyltransferase / mRNA (guanine-N7)-methyltransferase / viral nucleocapsid / mRNA 5'-cap (guanine-N7-)-methyltransferase activity / GTP binding / RNA binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Hu, L. / Prasad, B.V.V. | ||||||
![]() | ![]() Title: Structural basis for 2'-5'-oligoadenylate binding and enzyme activity of a viral RNase L antagonist. Authors: Ogden, K.M. / Hu, L. / Jha, B.K. / Sankaran, B. / Weiss, S.R. / Silverman, R.H. / Patton, J.T. / Prasad, B.V. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 77.6 KB | Display | ![]() |
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PDB format | ![]() | 58.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 457.1 KB | Display | ![]() |
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Full document | ![]() | 459.3 KB | Display | |
Data in XML | ![]() | 16.2 KB | Display | |
Data in CIF | ![]() | 23.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 16766.479 Da / Num. of mol.: 2 / Fragment: C-terminal domain, UNP residues 694-835 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.06 Å3/Da / Density % sol: 40.23 % |
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Crystal grow | Temperature: 298 K / pH: 4 Details: 14% PEG3350, 0.2 M sodium malonate, pH 4, 1 M NaI, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
-Data collection
Diffraction | Mean temperature: 95 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Apr 28, 2014 |
Radiation | Monochromator: SI(220) ASYMMETRIC CUT SINGLE CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9774 Å / Relative weight: 1 |
Reflection | Resolution: 1.35→31.86 Å / Num. obs: 56612 / % possible obs: 95.2 % / Observed criterion σ(I): 2 / Redundancy: 4.9 % / Rmerge(I) obs: 0.039 / Rsym value: 0.044 / Net I/σ(I): 19.2 |
Reflection shell | Resolution: 1.35→1.42 Å / Rmerge(I) obs: 0.471 / Mean I/σ(I) obs: 3.2 / Rsym value: 0.528 / % possible all: 93.5 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.35→31.86 Å
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Refine LS restraints |
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LS refinement shell |
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