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Open data
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Basic information
Entry | Database: PDB / ID: 4r0g | ||||||
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Title | Crystal structure of Lpg0393 from Legionella pneumophila | ||||||
![]() | Uncharacterized protein | ||||||
![]() | UNKNOWN FUNCTION / Vps9 / GEF | ||||||
Function / homology | Lpg0393, helical bundle domain / Helical bundle domain / HBD domain-containing protein![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Sohn, Y.S. / Shin, H.C. / Oh, B.H. | ||||||
![]() | ![]() Title: Lpg0393 of Legionella pneumophila Is a Guanine-Nucleotide Exchange Factor for Rab5, Rab21 and Rab22 Authors: Sohn, Y.S. / Shin, H.C. / Park, W.S. / Ge, J. / Kim, C.H. / Lee, B.L. / Heo, W.D. / Jung, J.U. / Rigden, D.J. / Oh, B.H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 203.8 KB | Display | ![]() |
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PDB format | ![]() | 166.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 432.2 KB | Display | ![]() |
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Full document | ![]() | 449.6 KB | Display | |
Data in XML | ![]() | 21.2 KB | Display | |
Data in CIF | ![]() | 32 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Details | AUTHOR DETERMINED BIOLOGICAL ASSEMBLY: UNKNOWN |
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Components
#1: Protein | Mass: 33457.371 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: lpg0393 / Plasmid: pET22b / Production host: ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.79 Å3/Da / Density % sol: 55.93 % |
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Crystal grow | Temperature: 295.15 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 8% PEG 8000, 0.1M HEPES, 8% ethylene glycol, 13% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K |
-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Apr 22, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97918 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→50 Å / Num. all: 61472 / Num. obs: 43800 / % possible obs: 99.7 % / Redundancy: 6.1 % / Rsym value: 0.057 / Net I/σ(I): 24.5 |
Reflection shell | Resolution: 2.7→2.75 Å / Redundancy: 4.7 % / Mean I/σ(I) obs: 2.7 / % possible all: 99.7 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 170.96 Å2 / Biso mean: 79.96 Å2 / Biso min: 30.24 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.7→34.039 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 14
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