+Open data
-Basic information
Entry | Database: PDB / ID: 4qyw | ||||||
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Title | Structure of phosphono-CheY from T.maritima | ||||||
Components | Chemotaxis protein CheY | ||||||
Keywords | SIGNALING PROTEIN / phosphono-CheY / alpha beta globular protein | ||||||
Function / homology | Function and homology information archaeal or bacterial-type flagellum-dependent cell motility / phosphorelay signal transduction system / chemotaxis / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | Thermotoga maritima (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.597 Å | ||||||
Authors | Beyersdorf, M.S. / Sircar, R. / Crane, B.R. / Halkides, C.J. | ||||||
Citation | Journal: To be Published Title: Isolation, Phosphonomethylation, and Crystallization of phosphono-CheY from Thermotoga maritima Authors: Beyersdorf, M.S. / Roebuck, J.A. / Sircar, R. / Pavlovsky, A. / Crane, B.R. / Halkides, C.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4qyw.cif.gz | 32.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4qyw.ent.gz | 25 KB | Display | PDB format |
PDBx/mmJSON format | 4qyw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/qy/4qyw ftp://data.pdbj.org/pub/pdb/validation_reports/qy/4qyw | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 13153.555 Da / Num. of mol.: 1 / Mutation: D54(CYQ), C81A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermotoga maritima (bacteria) / Gene: cheY, TM_0700 / Production host: Escherichia coli (E. coli) / References: UniProt: Q56312 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.92 Å3/Da / Density % sol: 36.04 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 5.5 Details: 100mM acetate, pH 5.5, 350 uM Sc(ClO4)3, 0.02% azide, 150 mM sodium malonate, 19% PeEG 6000., VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: CHESS / Beamline: A1 / Wavelength: 0.97 Å |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD |
Radiation | Monochromator: Si III channel / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97 Å / Relative weight: 1 |
Reflection | Resolution: 1.59→23.032 Å / Num. all: 13650 / Num. obs: 13499 / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.597→23.032 Å / SU ML: 0.14 / σ(F): 0 / σ(I): -3 / Phase error: 28.49 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.597→23.032 Å
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Refine LS restraints |
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LS refinement shell |
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