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- PDB-4qts: Crystal structure of Csm3-Csm4 subcomplex in the type III-A CRISP... -
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Basic information
Entry | Database: PDB / ID: 4qts | ||||||
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Title | Crystal structure of Csm3-Csm4 subcomplex in the type III-A CRISPR-Cas interference complex | ||||||
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![]() | RNA BINDING PROTEIN / CRISPR-associated protein / ferredoxin-like fold / Type III-A CRISPR-Cas system | ||||||
Function / homology | ![]() endonuclease activity / defense response to virus / Hydrolases; Acting on ester bonds / RNA binding / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Numata, T. / Inanaga, H. / Osawa, T. | ||||||
![]() | ![]() Title: Crystal structure of the Csm3-Csm4 subcomplex in the type III-A CRISPR-Cas interference complex. Authors: Numata, T. / Inanaga, H. / Sato, C. / Osawa, T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 212.3 KB | Display | ![]() |
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PDB format | ![]() | 168.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 43922.531 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: csm4, MJ1668 / Plasmid: pET28b / Production host: ![]() ![]() #2: Protein | Mass: 30377.734 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: csm3, MJ1669 / Plasmid: pET28b / Production host: ![]() ![]() #3: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.36 Å3/Da / Density % sol: 47.8 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.8 Details: 50mM HEPES-Na (pH 6.8), 200mM potassium chrolide, 30% v/v Pentaerythritol propoxylate (5/4 PO/OH), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 95 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jun 11, 2012 |
Radiation | Monochromator: Numerical link type Si(111) double crystal monochromator, liquid nitrogen cooling Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 3.1→50 Å / Num. obs: 25906 / % possible obs: 99.2 % / Redundancy: 3.8 % / Rmerge(I) obs: 0.058 / Net I/σ(I): 15.1 |
Reflection shell | Resolution: 3.1→3.29 Å / Redundancy: 3.9 % / Rmerge(I) obs: 0.557 / Mean I/σ(I) obs: 2.7 / Num. unique all: 30283 / % possible all: 99.4 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.105→43.46 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 9
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