- PDB-4qmk: Crystal structure of type III effector protein ExoU (exoU) -
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Basic information
Entry
Database: PDB / ID: 4qmk
Title
Crystal structure of type III effector protein ExoU (exoU)
Components
Type III secretion system effector protein ExoU
Keywords
TOXIN / type III secretion system / Pseudomonas fluorescens A506 / type III effector protein ExoU / Infectious Diseases / Structural Genomics / Center for Structural Genomics of Infectious Diseases / CSGID / NIAID / National Institute of Allergy and Infectious Diseases / membrane localization domain / PLA2 domain / patatin-like phospholipase domain
Mass: 18.015 Da / Num. of mol.: 206 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.3 Å3/Da / Density % sol: 46.46 %
Crystal grow
Temperature: 295 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: protein: 5.8 mg/ml in 10 mM Tris-HCl pH 8.3, 500 mM NaCl, 5 mM BME. Crystallization conditions: The Classics II Suite (#G7:79): 0.2 M Ammonium acetate, 0.1 M Bis-Tris (pH 6.5), 25% (w/v) PEG ...Details: protein: 5.8 mg/ml in 10 mM Tris-HCl pH 8.3, 500 mM NaCl, 5 mM BME. Crystallization conditions: The Classics II Suite (#G7:79): 0.2 M Ammonium acetate, 0.1 M Bis-Tris (pH 6.5), 25% (w/v) PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K
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