- PDB-4q25: Crystal structure of PhoU from Pseudomonas aeruginosa -
+
Open data
ID or keywords:
Loading...
-
Basic information
Entry
Database: PDB / ID: 4q25
Title
Crystal structure of PhoU from Pseudomonas aeruginosa
Components
Phosphate-specific transport system accessory protein PhoU homolog
Keywords
UNKNOWN FUNCTION / Helix bundle / Membrane
Function / homology
Function and homology information
negative regulation of phosphate transmembrane transport / negative regulation of positive chemotaxis / negative regulation of phosphate metabolic process / negative regulation of chemotaxis / phosphate ion transport / intracellular phosphate ion homeostasis / cell chemotaxis / protein homodimerization activity / cytoplasm Similarity search - Function
Phosphate transport system protein PhoU / PhoU domain / PhoU domain / Phosphate transport system protein phou homolog 2; domain 2 / PhoU-like domain superfamily / Methane Monooxygenase Hydroxylase; Chain G, domain 1 / Up-down Bundle / Mainly Alpha Similarity search - Domain/homology
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 1 Å / Relative weight: 1
Reflection
Resolution: 2.28→50 Å / Num. obs: 22783 / % possible obs: 99.9 %
Reflection shell
Resolution: 2.28→2.32 Å / % possible all: 98.8
-
Processing
Software
Name
Version
Classification
HKL-2000
datacollection
SOLVE
phasing
REFMAC
5.7.0029
refinement
HKL-2000
datareduction
HKL-2000
datascaling
Refinement
Method to determine structure: SAD / Resolution: 2.28→49.89 Å / Cor.coef. Fo:Fc: 0.951 / Cor.coef. Fo:Fc free: 0.942 / SU B: 5.957 / SU ML: 0.146 / Cross valid method: THROUGHOUT / ESU R: 0.318 / ESU R Free: 0.217 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.23426
1170
5.1 %
RANDOM
Rwork
0.20915
-
-
-
obs
0.21041
21595
97.03 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK