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- PDB-4q0s: Crystal structure of Acinetobacter sp. DL28 L-ribose isomerase in... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4q0s | ||||||
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Title | Crystal structure of Acinetobacter sp. DL28 L-ribose isomerase in complex with ribitol | ||||||
![]() | L-Ribose isomerase | ||||||
![]() | ISOMERASE / Cupin barrel / Sugar binding | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Yoshida, H. / Yoshihara, A. / Teraoka, M. / Izumori, K. / Kamitori, S. | ||||||
![]() | ![]() Title: X-ray structure of a novel L-ribose isomerase acting on a non-natural sugar L-ribose as its ideal substrate. Authors: Yoshida, H. / Yoshihara, A. / Teraoka, M. / Terami, Y. / Takata, G. / Izumori, K. / Kamitori, S. #1: Journal: Acta Crystallogr.,Sect.F / Year: 2011 Title: Overexpression, crystallization and preliminary X-ray diffraction analysis of L-ribose isomerase from Acinetobacter sp. strain DL-28 Authors: Yoshida, H. / Teraoka, M. / Yoshihara, A. / Izumori, K. / Kamitori, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 67 KB | Display | ![]() |
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PDB format | ![]() | 47.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 458 KB | Display | ![]() |
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Full document | ![]() | 461.7 KB | Display | |
Data in XML | ![]() | 13 KB | Display | |
Data in CIF | ![]() | 17.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4q0pSC ![]() 4q0qC ![]() 4q0uC ![]() 4q0vC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 28751.254 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: Q93UQ5, Isomerases; Intramolecular oxidoreductases; Interconverting aldoses and ketoses, and related compounds | ||||
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#2: Chemical | ChemComp-CO / | ||||
#3: Chemical | #4: Chemical | ChemComp-NCO / | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.64 Å3/Da / Density % sol: 53.46 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 10-16%(v/v) PEG400, 0.2M NaCl, 0.1M HEPES, 0.02M Hexaammine cobalt(III) chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS VII / Detector: IMAGE PLATE / Date: Apr 26, 2012 / Details: Mirrors |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.93→22.31 Å / Num. all: 22982 / Num. obs: 22982 / % possible obs: 99.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4.6 % / Biso Wilson estimate: 24.6 Å2 / Rmerge(I) obs: 0.059 / Net I/σ(I): 12.3 |
Reflection shell | Resolution: 1.93→2 Å / Redundancy: 4.5 % / Rmerge(I) obs: 0.352 / Mean I/σ(I) obs: 3.8 / Num. unique all: 2277 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 4Q0P Resolution: 1.93→22.31 Å / Rfactor Rfree error: 0.005 / Data cutoff high absF: 6935695.1 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber / Details: BULK SOLVENT MODEL USED
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 59.3269 Å2 / ksol: 0.45 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 36.4 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.93→22.31 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.93→2 Å / Rfactor Rfree error: 0.022 / Total num. of bins used: 10
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Xplor file |
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