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Open data
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Basic information
Entry | Database: PDB / ID: 4pqj | ||||||
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Title | Crystal structure of a phosphate binding protein | ||||||
![]() | Phosphate binding protein | ||||||
![]() | TRANSPORT PROTEIN / psts / phosphate binding / biofilm / substrate binding protein / phosphate transport | ||||||
Function / homology | ![]() phosphate ion transport / single-species biofilm formation / phosphate ion binding / periplasmic space / cell adhesion / extracellular region Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Opatowsky, Y. / Neznansky, A. | ||||||
![]() | ![]() Title: Crystal structure of a phosphate binding protein Authors: Opatowsky, Y. / Neznansky, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 118.7 KB | Display | ![]() |
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PDB format | ![]() | 97 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 448.3 KB | Display | ![]() |
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Full document | ![]() | 454.3 KB | Display | |
Data in XML | ![]() | 25.7 KB | Display | |
Data in CIF | ![]() | 37.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 32176.600 Da / Num. of mol.: 2 / Fragment: UNP residues 25-323 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.16 Å3/Da / Density % sol: 43.12 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 25% PEG 3350, 0.1M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K | |||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||||||||
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Jul 20, 2011 | |||||||||||||||||||||
Radiation | Monochromator: Si 111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||
Radiation wavelength | Wavelength: 0.9763 Å / Relative weight: 1 | |||||||||||||||||||||
Reflection | Resolution: 1.86→62.16 Å / Num. all: 47406 / Num. obs: 47406 / % possible obs: 100 % / Observed criterion σ(F): 94280 / Observed criterion σ(I): 94280 / Redundancy: 2 % / Biso Wilson estimate: 24.67 Å2 / Rmerge(I) obs: 0.097 / Net I/σ(I): 5.08 | |||||||||||||||||||||
Reflection shell |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.86→62.159 Å
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Refine LS restraints |
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LS refinement shell |
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