- PDB-4ph4: The crystal structure of Human VPS34 in complex with PIK-III -
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基本情報
登録情報
データベース: PDB / ID: 4ph4
タイトル
The crystal structure of Human VPS34 in complex with PIK-III
要素
Phosphatidylinositol 3-kinase catalytic subunit type 3
キーワード
TRANSFERASE/TRANSFERASE INHIBITOR / VPS34 / autophagy / class III / phosphatidylinositol-3-kinase / PIK3C3 / TRANSFERASE-TRANSFERASE INHIBITOR complex
機能・相同性
機能・相同性情報
Toll Like Receptor 9 (TLR9) Cascade / protein lipidation / Synthesis of PIPs at the late endosome membrane / phosphatidylinositol 3-kinase complex, class III / Synthesis of PIPs at the early endosome membrane / phosphatidylinositol 3-kinase complex, class III, type II / phosphatidylinositol 3-kinase complex, class III, type I / positive regulation by host of viral genome replication / Synthesis of PIPs at the Golgi membrane / phosphatidylinositol kinase activity ...Toll Like Receptor 9 (TLR9) Cascade / protein lipidation / Synthesis of PIPs at the late endosome membrane / phosphatidylinositol 3-kinase complex, class III / Synthesis of PIPs at the early endosome membrane / phosphatidylinositol 3-kinase complex, class III, type II / phosphatidylinositol 3-kinase complex, class III, type I / positive regulation by host of viral genome replication / Synthesis of PIPs at the Golgi membrane / phosphatidylinositol kinase activity / protein localization to phagophore assembly site / protein targeting to lysosome / early endosome to late endosome transport / Translation of Replicase and Assembly of the Replication Transcription Complex / phagophore assembly site / autolysosome / phosphatidylinositol 3-kinase / phosphatidylinositol-3-phosphate biosynthetic process / Macroautophagy / 1-phosphatidylinositol-3-kinase activity / axoneme / phosphatidylinositol-mediated signaling / phosphatidylinositol phosphate biosynthetic process / autophagosome maturation / autophagosome assembly / PI3K Cascade / RHO GTPases Activate NADPH Oxidases / autophagosome / regulation of macroautophagy / cellular response to glucose starvation / regulation of cytokinesis / regulation of autophagy / macroautophagy / Antigen Presentation: Folding, assembly and peptide loading of class I MHC / autophagy / endocytosis / phagocytic vesicle membrane / peroxisome / late endosome / kinase activity / Translation of Replicase and Assembly of the Replication Transcription Complex / midbody / protein kinase activity / endosome / cell cycle / phosphorylation / cell division / SARS-CoV-2 activates/modulates innate and adaptive immune responses / ATP binding / membrane / cytoplasm / cytosol 類似検索 - 分子機能
温度: 303.15 K / 手法: 蒸気拡散法, ハンギングドロップ法 詳細: VPS34 protein and PIK-III were mixed and incubated on ice for 1 hr (final PIK-III concentration was 1 mM). Prior to crystallization, the mixture was passed through a 0.2 um filter. The ...詳細: VPS34 protein and PIK-III were mixed and incubated on ice for 1 hr (final PIK-III concentration was 1 mM). Prior to crystallization, the mixture was passed through a 0.2 um filter. The protein:ligand complex was crystallized using the hanging drop vapor diffusion method in Nextal plates: 6 uL of protein solution was mixed with 4 uL of precipitant, which consisted of 20% (w/v) PEG 3350, 100 mM bis-tris propane, and 200 mM Na-K-phosphate. The resulting drop was suspended over a reservoir of 0.3 mL of precipitant and sealed with a screw cap. The crystals grew at 30 degC in approximately 12-24 hr.
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データ収集
回折
平均測定温度: 100 K
放射光源
由来: シンクロトロン / サイト: ALS / ビームライン: 5.0.2 / 波長: 0.9774 Å
検出器
タイプ: ADSC QUANTUM 315 / 検出器: CCD / 日付: 2009年1月21日
放射
モノクロメーター: Double-crystal, Si(111) liquid N2 cooled プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 0.9774 Å / 相対比: 1
反射
解像度: 1.98→48.75 Å / Num. obs: 29404 / % possible obs: 100 % / 冗長度: 15.4 % / Biso Wilson estimate: 85.63 Å2 / Net I/σ(I): 20.9
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位相決定
位相決定
手法: 分子置換
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解析
ソフトウェア
名称
バージョン
分類
XDS
データ削減
BUSTER-TNT
BUSTER2.11.4
精密化
PDB_EXTRACT
3.14
データ抽出
PHASER
位相決定
BUSTER
2.11.4
精密化
精密化
構造決定の手法: 分子置換 / 解像度: 2.8→48.34 Å / Cor.coef. Fo:Fc: 0.9466 / Cor.coef. Fo:Fc free: 0.9285 / SU R Cruickshank DPI: 0.423 / 交差検証法: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.438 / SU Rfree Blow DPI: 0.262 / SU Rfree Cruickshank DPI: 0.263